Restriction map for
pFN21AE3205
(Linear) (Six-base) MAPSORT of: KIEE3205.seq Check: 373 from: 1 to: 1428
KIEE3205.orf
With 182 enzymes: SgfI *
March 22, 2012 16:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 462 1428
Size: 301 161 966
Fragments arranged by size:
966 301 161
AflIII A'CryG_T
Cuts at: 0 965 1428
Size: 965 463
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 952 984 1428
Size: 952 32 444
Fragments arranged by size:
952 444 32
AlwNI CAG_nnn'CTG
Cuts at: 0 227 330 403 546 1080 1125 1320 1428
Size: 227 103 73 143 534 45 195 108
Fragments arranged by size:
534 227 195 143 108 103 73 45
ApaLI G'TGCA_C
Cuts at: 0 442 1428
Size: 442 986
AvaI C'yCGr_G
Cuts at: 0 945 983 1294 1428
Size: 945 38 311 134
Fragments arranged by size:
945 311 134 38
BanI G'GyrC_C
Cuts at: 0 312 904 1290 1428
Size: 312 592 386 138
Fragments arranged by size:
592 386 312 138
BanII G_rGCy'C
Cuts at: 0 661 923 946 992 1247 1428
Size: 661 262 23 46 255 181
Fragments arranged by size:
661 262 255 181 46 23
BbeI G_GCGC'C
Cuts at: 0 1294 1428
Size: 1294 134
BbsI GAAGACnn'nnnn_
Cuts at: 0 444 601 1428
Size: 444 157 827
Fragments arranged by size:
827 444 157
BbvCI CC'TCA_GC
Cuts at: 0 1010 1286 1428
Size: 1010 276 142
Fragments arranged by size:
1010 276 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1133 1167 1428
Size: 1133 34 261
Fragments arranged by size:
1133 261 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1190 1202 1428
Size: 1190 12 226
Fragments arranged by size:
1190 226 12
BglII A'GATC_T
Cuts at: 0 710 1428
Size: 710 718
Bme1580I G_kGCm'C
Cuts at: 0 446 1345 1428
Size: 446 899 83
Fragments arranged by size:
899 446 83
BmgBI CAC'GTC
Cuts at: 0 720 1428
Size: 720 708
BmrI ACTGGGnnnn_n'
Cuts at: 0 311 1428
Size: 311 1117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 873 1264 1428
Size: 873 391 164
Fragments arranged by size:
873 391 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 508 744 862 979 1081 1201 1234
Size: 202 306 236 118 117 102 120 33
Cuts at: 1234 1428
Size: 194
Fragments arranged by size:
306 236 202 194 120 118 117 102
33
Bpu10I CC'TnA_GC
Cuts at: 0 1010 1286 1428
Size: 1010 276 142
Fragments arranged by size:
1010 276 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 1428
Size: 453 975
BsaI GGTCTCn'nnnn_
Cuts at: 0 201 238 351 1428
Size: 201 37 113 1077
Fragments arranged by size:
1077 201 113 37
BsaAI yAC'GTr
Cuts at: 0 966 1167 1257 1428
Size: 966 201 90 171
Fragments arranged by size:
966 201 171 90
BsaHI Gr'CG_yC
Cuts at: 0 4 854 1291 1428
Size: 4 850 437 137
Fragments arranged by size:
850 437 137 4
BsaWI w'CCGG_w
Cuts at: 0 1421 1428
Size: 1421 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 952 982 1252 1282 1428
Size: 952 30 270 30 146
Fragments arranged by size:
952 270 146 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 931 1237 1428
Size: 289 642 306 191
Fragments arranged by size:
642 306 289 191
BseYI C'CCAG_C
Cuts at: 0 260 683 1234 1428
Size: 260 423 551 194
Fragments arranged by size:
551 423 260 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 359 918 1069 1150 1379 1428
Size: 240 119 559 151 81 229 49
Fragments arranged by size:
559 240 229 151 119 81 49
BsiHKAI G_wGCw'C
Cuts at: 0 446 788 965 992 1247 1428
Size: 446 342 177 27 255 181
Fragments arranged by size:
446 342 255 181 177 27
BsiWI C'GTAC_G
Cuts at: 0 1100 1428
Size: 1100 328
BsmBI CGTCTCn'nnnn_
Cuts at: 0 65 861 1428
Size: 65 796 567
Fragments arranged by size:
796 567 65
Bsp1286I G_dGCh'C
Cuts at: 0 446 661 788 923 946 965 992 1247
Size: 446 215 127 135 23 19 27 255
Cuts at: 1247 1345 1428
Size: 98 83
Fragments arranged by size:
446 255 215 135 127 98 83 27
23 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 639 1351 1428
Size: 639 712 77
Fragments arranged by size:
712 639 77
BsrDI GCAATG_nn'
Cuts at: 0 1100 1428
Size: 1100 328
BsrGI T'GTAC_A
Cuts at: 0 1322 1428
Size: 1322 106
BssSI C'ACGA_G
Cuts at: 0 271 1428
Size: 271 1157
BstAPI GCAn_nnn'nTGC
Cuts at: 0 227 1428
Size: 227 1201
BstEII G'GTnAC_C
Cuts at: 0 1014 1428
Size: 1014 414
BstYI r'GATC_y
Cuts at: 0 578 710 1428
Size: 578 132 718
Fragments arranged by size:
718 578 132
Bsu36I CC'TnA_GG
Cuts at: 0 1030 1188 1428
Size: 1030 158 240
Fragments arranged by size:
1030 240 158
BtgI C'CryG_G
Cuts at: 0 1391 1428
Size: 1391 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 350 1428
Size: 350 1078
DraI TTT'AAA
Cuts at: 0 429 1428
Size: 429 999
DraIII CAC_nnn'GTG
Cuts at: 0 1167 1392 1428
Size: 1167 225 36
Fragments arranged by size:
1167 225 36
EaeI y'GGCC_r
Cuts at: 0 113 1428
Size: 113 1315
EarI CTCTTCn'nnn_
Cuts at: 0 842 1428
Size: 842 586
EcoICRI GAG'CTC
Cuts at: 0 990 1245 1428
Size: 990 255 183
Fragments arranged by size:
990 255 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 301 462 508 744 862 979 1081
Size: 202 99 161 46 236 118 117 102
Cuts at: 1081 1201 1234 1428
Size: 120 33 194
Fragments arranged by size:
236 202 194 161 120 118 117 102
99 46 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 468 667 1428
Size: 468 199 761
Fragments arranged by size:
761 468 199
EcoO109I rG'GnC_Cy
Cuts at: 0 170 1221 1428
Size: 170 1051 207
Fragments arranged by size:
1051 207 170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 693 1428
Size: 693 735
HaeII r_GCGC'y
Cuts at: 0 25 547 1152 1294 1428
Size: 25 522 605 142 134
Fragments arranged by size:
605 522 142 134 25
KasI G'GCGC_C
Cuts at: 0 1290 1428
Size: 1290 138
MscI TGG'CCA
Cuts at: 0 115 1428
Size: 115 1313
MslI CAynn'nnrTG
Cuts at: 0 727 1428
Size: 727 701
MspA1I CmG'CkG
Cuts at: 0 17 30 224 387 902 1428
Size: 17 13 194 163 515 526
Fragments arranged by size:
526 515 194 163 17 13
NarI GG'CG_CC
Cuts at: 0 1291 1428
Size: 1291 137
PflMI CCAn_nnn'nTGG
Cuts at: 0 1125 1167 1257 1392 1428
Size: 1125 42 90 135 36
Fragments arranged by size:
1125 135 90 42 36
PfoI T'CCnGG_A
Cuts at: 0 485 1046 1428
Size: 485 561 382
Fragments arranged by size:
561 485 382
PmlI CAC'GTG
Cuts at: 0 966 1257 1428
Size: 966 291 171
Fragments arranged by size:
966 291 171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 952 984 1428
Size: 952 32 444
Fragments arranged by size:
952 444 32
PpuMI rG'GwC_Cy
Cuts at: 0 170 1428
Size: 170 1258
PstI C_TGCA'G
Cuts at: 0 29 538 915 1340 1428
Size: 29 509 377 425 88
Fragments arranged by size:
509 425 377 88 29
PvuII CAG'CTG
Cuts at: 0 30 387 1428
Size: 30 357 1041
Fragments arranged by size:
1041 357 30
SacI G_AGCT'C
Cuts at: 0 992 1247 1428
Size: 992 255 181
Fragments arranged by size:
992 255 181
SanDI GG'GwC_CC
Cuts at: 0 170 1428
Size: 170 1258
SexAI A'CCwGG_T
Cuts at: 0 1124 1428
Size: 1124 304
SfcI C'TryA_G
Cuts at: 0 25 395 534 911 1336 1428
Size: 25 370 139 377 425 92
Fragments arranged by size:
425 377 370 139 92 25
SfoI GGC'GCC
Cuts at: 0 1292 1428
Size: 1292 136
SmlI C'TyrA_G
Cuts at: 0 468 1428
Size: 468 960
StyI C'CwwG_G
Cuts at: 0 191 1428
Size: 191 1237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 274 330 333 1428
Size: 274 56 3 1095
Fragments arranged by size:
1095 274 56 3
TatI w'GTAC_w
Cuts at: 0 1322 1428
Size: 1322 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 95 127 880 912 989 1021 1339 1371
Size: 95 32 753 32 77 32 318 32
Cuts at: 1371 1428
Size: 57
Fragments arranged by size:
753 318 95 77 57 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 808 1428
Size: 808 620
XbaI T'CTAG_A
Cuts at: 0 291 358 1428
Size: 291 67 1070
Fragments arranged by size:
1070 291 67
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1410 1428
Size: 1410 18
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApaLI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI
Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI BstEII BstYI
Bsu36I BtgI BtgZI DraI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII KasI MscI MslI
MspA1I NarI PflMI PfoI PmlI PpiI PpuMI PstI
PvuII SacI SanDI SexAI SfcI SfoI SmlI StyI
TaqII TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BlpI BmtI
BsaBI BsiEI BsmI BspEI BspHI BsrBI BsrFI BssHII
BstBI BstXI BstZ17I BtsI ClaI CspCI DrdI EagI
EciI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XhoI XmaI XmnI ZraI