Restriction map for
pFN21AE3204
(Linear) (Six-base) MAPSORT of: KIEE3204.seq Check: 9054 from: 1 to: 1419
KIEE3204.orf
With 182 enzymes: SgfI *
March 22, 2012 16:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 672 1419
Size: 672 747
AccI GT'mk_AC
Cuts at: 0 4 1419
Size: 4 1415
AclI AA'CG_TT
Cuts at: 0 207 1419
Size: 207 1212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 295 616 697 925 1093 1419
Size: 287 8 321 81 228 168 326
Fragments arranged by size:
326 321 287 228 168 81 8
AlwNI CAG_nnn'CTG
Cuts at: 0 816 931 960 1419
Size: 816 115 29 459
Fragments arranged by size:
816 459 115 29
ApaI G_GGCC'C
Cuts at: 0 1153 1419
Size: 1153 266
ApoI r'AATT_y
Cuts at: 0 154 691 1132 1419
Size: 154 537 441 287
Fragments arranged by size:
537 441 287 154
AseI AT'TA_AT
Cuts at: 0 580 1419
Size: 580 839
AvrII C'CTAG_G
Cuts at: 0 543 1419
Size: 543 876
BanI G'GyrC_C
Cuts at: 0 839 1419
Size: 839 580
BanII G_rGCy'C
Cuts at: 0 1153 1417 1419
Size: 1153 264 2
Fragments arranged by size:
1153 264 2
BbsI GAAGACnn'nnnn_
Cuts at: 0 557 784 1419
Size: 557 227 635
Fragments arranged by size:
635 557 227
BbvCI CC'TCA_GC
Cuts at: 0 1333 1419
Size: 1333 86
BciVI GTATCCnnnnn_n'
Cuts at: 0 1211 1419
Size: 1211 208
BfrBI ATG'CAT
Cuts at: 0 1305 1419
Size: 1305 114
BglII A'GATC_T
Cuts at: 0 900 1419
Size: 900 519
Bme1580I G_kGCm'C
Cuts at: 0 842 1153 1419
Size: 842 311 266
Fragments arranged by size:
842 311 266
BmrI ACTGGGnnnn_n'
Cuts at: 0 50 1419
Size: 50 1369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 128 212 838 1419
Size: 118 10 84 626 581
Fragments arranged by size:
626 581 118 84 10
Bpu10I CC'TnA_GC
Cuts at: 0 48 178 754 1333 1419
Size: 48 130 576 579 86
Fragments arranged by size:
579 576 130 86 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 1193 1419
Size: 1193 226
BsaBI GATnn'nnATC
Cuts at: 0 533 1419
Size: 533 886
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1128 1419
Size: 1128 291
BseYI C'CCAG_C
Cuts at: 0 328 366 1419
Size: 328 38 1053
Fragments arranged by size:
1053 328 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 1419
Size: 1378 41
Bsp1286I G_dGCh'C
Cuts at: 0 842 1153 1417 1419
Size: 842 311 264 2
Fragments arranged by size:
842 311 264 2
BspHI T'CATG_A
Cuts at: 0 339 1419
Size: 339 1080
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 672 1419
Size: 98 574 747
Fragments arranged by size:
747 574 98
BsrBI CCG'CTC
Cuts at: 0 1371 1419
Size: 1371 48
BsrDI GCAATG_nn'
Cuts at: 0 434 1419
Size: 434 985
BstBI TT'CG_AA
Cuts at: 0 223 1419
Size: 223 1196
BstEII G'GTnAC_C
Cuts at: 0 289 1419
Size: 289 1130
BstYI r'GATC_y
Cuts at: 0 900 1419
Size: 900 519
BtgI C'CryG_G
Cuts at: 0 405 1419
Size: 405 1014
BtsI GCAGTG_nn'
Cuts at: 0 240 1419
Size: 240 1179
DraI TTT'AAA
Cuts at: 0 773 1419
Size: 773 646
EaeI y'GGCC_r
Cuts at: 0 408 741 1419
Size: 408 333 678
Fragments arranged by size:
678 408 333
EarI CTCTTCn'nnn_
Cuts at: 0 19 557 1099 1172 1419
Size: 19 538 542 73 247
Fragments arranged by size:
542 538 247 73 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 128 212 287 295 616 697 838
Size: 118 10 84 75 8 321 81 141
Cuts at: 838 925 1093 1419
Size: 87 168 326
Fragments arranged by size:
326 321 168 141 118 87 84 81
75 10 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1143 1419
Size: 1143 276
EcoO109I rG'GnC_Cy
Cuts at: 0 52 1149 1150 1419
Size: 52 1097 1 269
Fragments arranged by size:
1097 269 52 1
EcoRI G'AATT_C
Cuts at: 0 1132 1419
Size: 1132 287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 771 803 1004 1036 1171 1203 1419
Size: 771 32 201 32 135 32 216
Fragments arranged by size:
771 216 201 135 32 32 32
HincII GTy'rAC
Cuts at: 0 915 1419
Size: 915 504
MscI TGG'CCA
Cuts at: 0 410 743 1419
Size: 410 333 676
Fragments arranged by size:
676 410 333
MslI CAynn'nnrTG
Cuts at: 0 811 1419
Size: 811 608
MspA1I CmG'CkG
Cuts at: 0 1166 1377 1419
Size: 1166 211 42
Fragments arranged by size:
1166 211 42
NdeI CA'TA_TG
Cuts at: 0 999 1419
Size: 999 420
NsiI A_TGCA'T
Cuts at: 0 1307 1419
Size: 1307 112
PfoI T'CCnGG_A
Cuts at: 0 95 1419
Size: 95 1324
PpuMI rG'GwC_Cy
Cuts at: 0 52 1419
Size: 52 1367
PspOMI G'GGCC_C
Cuts at: 0 1149 1419
Size: 1149 270
PstI C_TGCA'G
Cuts at: 0 273 1419
Size: 273 1146
PvuII CAG'CTG
Cuts at: 0 1377 1419
Size: 1377 42
SfcI C'TryA_G
Cuts at: 0 269 1245 1419
Size: 269 976 174
Fragments arranged by size:
976 269 174
SspI AAT'ATT
Cuts at: 0 493 1419
Size: 493 926
StyI C'CwwG_G
Cuts at: 0 543 744 860 1419
Size: 543 201 116 559
Fragments arranged by size:
559 543 201 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1114 1419
Size: 1114 305
TatI w'GTAC_w
Cuts at: 0 1211 1419
Size: 1211 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 458 490 1419
Size: 458 32 929
Fragments arranged by size:
929 458 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1089 1419
Size: 1089 330
Enzymes that do cut:
AarI AccI AclI AcuI AlwNI ApaI ApoI AseI
AvrII BanI BanII BbsI BbvCI BciVI BfrBI BglII
Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BseRI BseYI
BsgI Bsp1286I BspHI BspMI BsrBI BsrDI BstBI BstEII
BstYI BtgI BtsI DraI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII MscI MslI MspA1I NdeI
NsiI PfoI PpuMI PspOMI PstI PvuII SfcI SspI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI AscI AsiSI AvaI BaeI BamHI
BbeI BcgI BclI BglI BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BsrFI BsrGI BssHII BssSI BstAPI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI