Restriction map for
pFN21AE3200
(Linear) (Six-base) MAPSORT of: KIEE3200.seq Check: 2522 from: 1 to: 6327
KIEE3200.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3873 5164 5305 6327
Size: 3873 1291 141 1022
Fragments arranged by size:
3873 1291 1022 141
AatII G_ACGT'C
Cuts at: 0 983 6327
Size: 983 5344
AccI GT'mk_AC
Cuts at: 0 290 2639 4778 6327
Size: 290 2349 2139 1549
Fragments arranged by size:
2349 2139 1549 290
Acc65I G'GTAC_C
Cuts at: 0 2423 4196 6327
Size: 2423 1773 2131
Fragments arranged by size:
2423 2131 1773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1027 1424 1637 1807 2582 2627 2833
Size: 532 495 397 213 170 775 45 206
Cuts at: 2833 2890 3679 4270 5723 5879 6327
Size: 57 789 591 1453 156 448
Fragments arranged by size:
1453 789 775 591 532 495 448 397
213 206 170 156 57 45
AfeI AGC'GCT
Cuts at: 0 68 5660 6148 6327
Size: 68 5592 488 179
Fragments arranged by size:
5592 488 179 68
AflIII A'CryG_T
Cuts at: 0 1688 2411 6327
Size: 1688 723 3916
Fragments arranged by size:
3916 1688 723
AgeI A'CCGG_T
Cuts at: 0 218 734 6327
Size: 218 516 5593
Fragments arranged by size:
5593 516 218
AleI CACnn'nnGTG
Cuts at: 0 3856 5047 6327
Size: 3856 1191 1280
Fragments arranged by size:
3856 1280 1191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3745 5065 6327
Size: 3745 1320 1262
Fragments arranged by size:
3745 1320 1262
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1072 1104 6327
Size: 1072 32 5223
Fragments arranged by size:
5223 1072 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1612 2865 2913 4185 4293 5379 6120 6327
Size: 1612 1253 48 1272 108 1086 741 207
Fragments arranged by size:
1612 1272 1253 1086 741 207 108 48
ApaI G_GGCC'C
Cuts at: 0 150 2233 3868 4462 4479 5696 6327
Size: 150 2083 1635 594 17 1217 631
Fragments arranged by size:
2083 1635 1217 631 594 150 17
ApaLI G'TGCA_C
Cuts at: 0 2074 6327
Size: 2074 4253
ApoI r'AATT_y
Cuts at: 0 248 641 1012 1148 2794 4105 4149 5673
Size: 248 393 371 136 1646 1311 44 1524
Cuts at: 5673 6327
Size: 654
Fragments arranged by size:
1646 1524 1311 654 393 371 248 136
44
AscI GG'CGCG_CC
Cuts at: 0 5586 6327
Size: 5586 741
AvaI C'yCGr_G
Cuts at: 0 162 188 5539 5570 6049 6327
Size: 162 26 5351 31 479 278
Fragments arranged by size:
5351 479 278 162 31 26
BamHI G'GATC_C
Cuts at: 0 501 4754 6317 6327
Size: 501 4253 1563 10
Fragments arranged by size:
4253 1563 501 10
BanI G'GyrC_C
Cuts at: 0 992 2423 2438 3068 3343 4196 4436 5550
Size: 992 1431 15 630 275 853 240 1114
Cuts at: 5550 5575 5629 6327
Size: 25 54 698
Fragments arranged by size:
1431 1114 992 853 698 630 275 240
54 25 15
BanII G_rGCy'C
Cuts at: 0 47 150 337 578 705 1355 2233 2709
Size: 47 103 187 241 127 650 878 476
Cuts at: 2709 3356 3560 3602 3868 3960 4462 4479 4655
Size: 647 204 42 266 92 502 17 176
Cuts at: 4655 5422 5696 5857 6058 6235 6254 6327
Size: 767 274 161 201 177 19 73
Fragments arranged by size:
878 767 650 647 502 476 274 266
241 204 201 187 177 176 161 127
103 92 73 47 42 19 17
BbeI G_GCGC'C
Cuts at: 0 3072 4440 6327
Size: 3072 1368 1887
Fragments arranged by size:
3072 1887 1368
BbsI GAAGACnn'nnnn_
Cuts at: 0 1259 1795 2216 5216 5474 5495 5510 5756
Size: 1259 536 421 3000 258 21 15 246
Cuts at: 5756 5786 5867 6327
Size: 30 81 460
Fragments arranged by size:
3000 1259 536 460 421 258 246 81
30 21 15
BbvCI CC'TCA_GC
Cuts at: 0 835 6327
Size: 835 5492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3544 3578 3739 3773 6327
Size: 3544 34 161 34 2554
Fragments arranged by size:
3544 2554 161 34 34
BclI T'GATC_A
Cuts at: 0 446 1859 1919 6327
Size: 446 1413 60 4408
Fragments arranged by size:
4408 1413 446 60
BglI GCCn_nnn'nGGC
Cuts at: 0 463 2551 2853 5058 5573 5596 6327
Size: 463 2088 302 2205 515 23 731
Fragments arranged by size:
2205 2088 731 515 463 302 23
BglII A'GATC_T
Cuts at: 0 5231 6327
Size: 5231 1096
BlpI GC'TnA_GC
Cuts at: 0 796 5827 6327
Size: 796 5031 500
Fragments arranged by size:
5031 796 500
Bme1580I G_kGCm'C
Cuts at: 0 150 2078 2192 2233 2441 3762 3868 4462
Size: 150 1928 114 41 208 1321 106 594
Cuts at: 4462 4479 5054 5578 5632 5696 6215 6327
Size: 17 575 524 54 64 519 112
Fragments arranged by size:
1928 1321 594 575 524 519 208 150
114 112 106 64 54 41 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 1802 2368 2671 6327
Size: 1802 566 303 3656
Fragments arranged by size:
3656 1802 566 303
BmtI G_CTAG'C
Cuts at: 0 907 6327
Size: 907 5420
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1074 2574 6327
Size: 1074 1500 3753
Fragments arranged by size:
3753 1500 1074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 58 103 196 562 719 1058 2168
Size: 31 27 45 93 366 157 339 1110
Cuts at: 2168 2809 3280 3301 3350 3451 4162 4712 4855
Size: 641 471 21 49 101 711 550 143
Cuts at: 4855 5164 5206 5383 5401 5530 5647 5831 6097
Size: 309 42 177 18 129 117 184 266
Cuts at: 6097 6124 6142 6201 6327
Size: 27 18 59 126
Fragments arranged by size:
1110 711 641 550 471 366 339 309
266 184 177 157 143 129 126 117
101 93 59 49 45 42 31 27
27 21 18 18
Bpu10I CC'TnA_GC
Cuts at: 0 835 1315 1806 5062 6327
Size: 835 480 491 3256 1265
Fragments arranged by size:
3256 1265 835 491 480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 3364 6327
Size: 982 2382 2963
Fragments arranged by size:
2963 2382 982
BsaI GGTCTCn'nnnn_
Cuts at: 0 578 2014 5608 6327
Size: 578 1436 3594 719
Fragments arranged by size:
3594 1436 719 578
BsaAI yAC'GTr
Cuts at: 0 4535 6327
Size: 4535 1792
BsaBI GATnn'nnATC
Cuts at: 0 2966 6327
Size: 2966 3361
BsaHI Gr'CG_yC
Cuts at: 0 980 1375 2519 3069 4437 5471 5621 6327
Size: 980 395 1144 550 1368 1034 150 706
Fragments arranged by size:
1368 1144 1034 980 706 550 395 150
BsaWI w'CCGG_w
Cuts at: 0 218 734 1751 2135 3617 6140 6327
Size: 218 516 1017 384 1482 2523 187
Fragments arranged by size:
2523 1482 1017 516 384 218 187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 1056 1086 2617 2647 3253 3283
Size: 4 30 1022 30 1531 30 606 30
Cuts at: 3283 6327
Size: 3044
Fragments arranged by size:
3044 1531 1022 606 30 30 30 30
4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 712 768 1063 1318 1402 1456 1459
Size: 196 516 56 295 255 84 54 3
Cuts at: 1459 1464 1759 2523 2716 3223 4434 5069 5182
Size: 5 295 764 193 507 1211 635 113
Cuts at: 5182 5293 6327
Size: 111 1034
Fragments arranged by size:
1211 1034 764 635 516 507 295 295
255 196 193 113 111 84 56 54
5 3
BseYI C'CCAG_C
Cuts at: 0 616 704 825 846 1523 2177 2702 3339
Size: 616 88 121 21 677 654 525 637
Cuts at: 3339 3569 4455 5594 5683 5739 6276 6327
Size: 230 886 1139 89 56 537 51
Fragments arranged by size:
1139 886 677 654 637 616 537 525
230 121 89 88 56 51 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 610 4542 5025 5425 6327
Size: 108 502 3932 483 400 902
Fragments arranged by size:
3932 902 502 483 400 108
BsiEI CG_ry'CG
Cuts at: 0 2527 5952 5956 6327
Size: 2527 3425 4 371
Fragments arranged by size:
3425 2527 371 4
BsiHKAI G_wGCw'C
Cuts at: 0 578 802 1355 2078 3560 3960 4306 6058
Size: 578 224 553 723 1482 400 346 1752
Cuts at: 6058 6327
Size: 269
Fragments arranged by size:
1752 1482 723 578 553 400 346 269
224
BsmI GAATG_Cn'
Cuts at: 0 1208 3000 4592 6327
Size: 1208 1792 1592 1735
Fragments arranged by size:
1792 1735 1592 1208
BsmBI CGTCTCn'nnnn_
Cuts at: 0 79 814 1680 6327
Size: 79 735 866 4647
Fragments arranged by size:
4647 866 735 79
Bsp1286I G_dGCh'C
Cuts at: 0 47 150 337 578 705 802 1355 2078
Size: 47 103 187 241 127 97 553 723
Cuts at: 2078 2192 2233 2441 2709 3356 3560 3602 3762
Size: 114 41 208 268 647 204 42 160
Cuts at: 3762 3868 3960 4306 4462 4479 4655 5054 5422
Size: 106 92 346 156 17 176 399 368
Cuts at: 5422 5578 5632 5696 5857 6058 6215 6235 6254
Size: 156 54 64 161 201 157 20 19
Cuts at: 6254 6327
Size: 73
Fragments arranged by size:
723 647 553 399 368 346 268 241
208 204 201 187 176 161 160 157
156 156 127 114 106 103 97 92
73 64 54 47 42 41 20 19
17
BspEI T'CCGG_A
Cuts at: 0 1751 2135 6140 6327
Size: 1751 384 4005 187
Fragments arranged by size:
4005 1751 384 187
BspHI T'CATG_A
Cuts at: 0 4049 6029 6327
Size: 4049 1980 298
Fragments arranged by size:
4049 1980 298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2115 3128 3509 3873 4917 4943 5164 5305
Size: 2115 1013 381 364 1044 26 221 141
Cuts at: 5305 6327
Size: 1022
Fragments arranged by size:
2115 1044 1022 1013 381 364 221 141
26
BsrBI CCG'CTC
Cuts at: 0 1095 5077 5831 6327
Size: 1095 3982 754 496
Fragments arranged by size:
3982 1095 754 496
BsrDI GCAATG_nn'
Cuts at: 0 3830 3846 6327
Size: 3830 16 2481
Fragments arranged by size:
3830 2481 16
BsrFI r'CCGG_y
Cuts at: 0 77 218 554 734 1319 3053 4433 4597
Size: 77 141 336 180 585 1734 1380 164
Cuts at: 4597 5951 6327
Size: 1354 376
Fragments arranged by size:
1734 1380 1354 585 376 336 180 164
141 77
BsrGI T'GTAC_A
Cuts at: 0 406 2867 6327
Size: 406 2461 3460
Fragments arranged by size:
3460 2461 406
BssHII G'CGCG_C
Cuts at: 0 5401 5586 6327
Size: 5401 185 741
Fragments arranged by size:
5401 741 185
BssSI C'ACGA_G
Cuts at: 0 2649 6295 6327
Size: 2649 3646 32
Fragments arranged by size:
3646 2649 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4556 5376 5448 6327
Size: 4556 820 72 879
Fragments arranged by size:
4556 879 820 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 71 5508 6327
Size: 71 5437 819
Fragments arranged by size:
5437 819 71
BstYI r'GATC_y
Cuts at: 0 501 935 2064 2138 3392 4754 5231 6317
Size: 501 434 1129 74 1254 1362 477 1086
Cuts at: 6317 6327
Size: 10
Fragments arranged by size:
1362 1254 1129 1086 501 477 434 74
10
Bsu36I CC'TnA_GG
Cuts at: 0 4144 6327
Size: 4144 2183
BtgI C'CryG_G
Cuts at: 0 2084 2456 2621 2945 3242 5252 5414 5956
Size: 2084 372 165 324 297 2010 162 542
Cuts at: 5956 6191 6236 6327
Size: 235 45 91
Fragments arranged by size:
2084 2010 542 372 324 297 235 165
162 91 45
BtsI GCAGTG_nn'
Cuts at: 0 596 3130 3434 5396 6327
Size: 596 2534 304 1962 931
Fragments arranged by size:
2534 1962 931 596 304
DraI TTT'AAA
Cuts at: 0 1017 4101 6327
Size: 1017 3084 2226
Fragments arranged by size:
3084 2226 1017
DraIII CAC_nnn'GTG
Cuts at: 0 1958 5448 6327
Size: 1958 3490 879
Fragments arranged by size:
3490 1958 879
DrdI GACnn_nn'nnGTC
Cuts at: 0 1051 3477 6327
Size: 1051 2426 2850
Fragments arranged by size:
2850 2426 1051
EaeI y'GGCC_r
Cuts at: 0 2524 4010 4706 5832 5949 5953 5973 6327
Size: 2524 1486 696 1126 117 4 20 354
Fragments arranged by size:
2524 1486 1126 696 354 117 20 4
EagI C'GGCC_G
Cuts at: 0 2524 5949 5953 6327
Size: 2524 3425 4 374
Fragments arranged by size:
3425 2524 374 4
EarI CTCTTCn'nnn_
Cuts at: 0 836 926 1613 3569 3911 3948 4053 4411
Size: 836 90 687 1956 342 37 105 358
Cuts at: 4411 4726 4847 5081 6184 6194 6327
Size: 315 121 234 1103 10 133
Fragments arranged by size:
1956 1103 836 687 358 342 315 234
133 121 105 90 37 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4348 6327
Size: 4348 1979
EcoICRI GAG'CTC
Cuts at: 0 576 1353 3558 3958 6056 6327
Size: 576 777 2205 400 2098 271
Fragments arranged by size:
2205 2098 777 576 400 271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 58 103 196 532 562 719 1027
Size: 31 27 45 93 336 30 157 308
Cuts at: 1027 1058 1424 1637 1807 2168 2582 2627 2809
Size: 31 366 213 170 361 414 45 182
Cuts at: 2809 2833 2890 3280 3301 3350 3451 3679 4162
Size: 24 57 390 21 49 101 228 483
Cuts at: 4162 4270 4712 4855 5164 5206 5383 5401 5530
Size: 108 442 143 309 42 177 18 129
Cuts at: 5530 5647 5723 5831 5879 6097 6124 6142 6201
Size: 117 76 108 48 218 27 18 59
Cuts at: 6201 6327
Size: 126
Fragments arranged by size:
483 442 414 390 366 361 336 309
308 228 218 213 182 177 170 157
143 129 126 117 108 108 101 93
76 59 57 49 48 45 45 42
31 31 30 27 27 24 21 18
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 426 1999 3063 3223 4012 4521 6327
Size: 426 1573 1064 160 789 509 1806
Fragments arranged by size:
1806 1573 1064 789 509 426 160
EcoO109I rG'GnC_Cy
Cuts at: 0 147 267 479 1478 2229 3666 3864 4459
Size: 147 120 212 999 751 1437 198 595
Cuts at: 4459 4476 5355 5724 6081 6117 6327
Size: 17 879 369 357 36 210
Fragments arranged by size:
1437 999 879 751 595 369 357 212
210 198 147 120 36 17
EcoRI G'AATT_C
Cuts at: 0 4149 6327
Size: 4149 2178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2487 2632 4440 6327
Size: 2487 145 1808 1887
Fragments arranged by size:
2487 1887 1808 145
FseI GG_CCGG'CC
Cuts at: 0 5955 6327
Size: 5955 372
FspI TGC'GCA
Cuts at: 0 4913 5287 6327
Size: 4913 374 1040
Fragments arranged by size:
4913 1040 374
HaeII r_GCGC'y
Cuts at: 0 70 3072 3242 3489 4440 4890 5662 6150
Size: 70 3002 170 247 951 450 772 488
Cuts at: 6150 6327
Size: 177
Fragments arranged by size:
3002 951 772 488 450 247 177 170
70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1056 1088 1512 1544 1911 1943 2632 2664
Size: 1056 32 424 32 367 32 689 32
Cuts at: 2664 3919 3951 4609 4641 6327
Size: 1255 32 658 32 1686
Fragments arranged by size:
1686 1255 1056 689 658 424 367 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2640 3009 4431 4446 5010 6303 6327
Size: 2640 369 1422 15 564 1293 24
Fragments arranged by size:
2640 1422 1293 564 369 24 15
HindIII A'AGCT_T
Cuts at: 0 781 2469 4117 4812 6327
Size: 781 1688 1648 695 1515
Fragments arranged by size:
1688 1648 1515 781 695
KasI G'GCGC_C
Cuts at: 0 3068 4436 6327
Size: 3068 1368 1891
Fragments arranged by size:
3068 1891 1368
KpnI G_GTAC'C
Cuts at: 0 2427 4200 6327
Size: 2427 1773 2127
Fragments arranged by size:
2427 2127 1773
MfeI C'AATT_G
Cuts at: 0 4211 6327
Size: 4211 2116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 1412 3927 4020 4586 6327
Size: 2 1410 2515 93 566 1741
Fragments arranged by size:
2515 1741 1410 566 93 2
MscI TGG'CCA
Cuts at: 0 5975 6327
Size: 5975 352
MslI CAynn'nnrTG
Cuts at: 0 301 772 1034 1735 3856 4741 4900 5047
Size: 301 471 262 701 2121 885 159 147
Cuts at: 5047 5635 6327
Size: 588 692
Fragments arranged by size:
2121 885 701 692 588 471 301 262
159 147
MspA1I CmG'CkG
Cuts at: 0 108 2256 2811 3768 5039 5071 5184 5594
Size: 108 2148 555 957 1271 32 113 410
Cuts at: 5594 5758 5764 5826 6327
Size: 164 6 62 501
Fragments arranged by size:
2148 1271 957 555 501 410 164 113
108 62 32 6
NaeI GCC'GGC
Cuts at: 0 79 1321 3055 4599 5953 6327
Size: 79 1242 1734 1544 1354 374
Fragments arranged by size:
1734 1544 1354 1242 374 79
NarI GG'CG_CC
Cuts at: 0 3069 4437 6327
Size: 3069 1368 1890
Fragments arranged by size:
3069 1890 1368
NcoI C'CATG_G
Cuts at: 0 2084 3242 5414 6236 6327
Size: 2084 1158 2172 822 91
Fragments arranged by size:
2172 2084 1158 822 91
NdeI CA'TA_TG
Cuts at: 0 302 6327
Size: 302 6025
NgoMIV G'CCGG_C
Cuts at: 0 77 1319 3053 4597 5951 6327
Size: 77 1242 1734 1544 1354 376
Fragments arranged by size:
1734 1544 1354 1242 376 77
NheI G'CTAG_C
Cuts at: 0 903 6327
Size: 903 5424
NspI r_CATG'y
Cuts at: 0 2415 3907 6327
Size: 2415 1492 2420
Fragments arranged by size:
2420 2415 1492
PasI CC'CwG_GG
Cuts at: 0 3670 6327
Size: 3670 2657
PciI A'CATG_T
Cuts at: 0 2411 6327
Size: 2411 3916
PflMI CCAn_nnn'nTGG
Cuts at: 0 4278 4455 4578 6120 6327
Size: 4278 177 123 1542 207
Fragments arranged by size:
4278 1542 207 177 123
PfoI T'CCnGG_A
Cuts at: 0 212 539 2352 4178 4757 5360 5663 6327
Size: 212 327 1813 1826 579 603 303 664
Fragments arranged by size:
1826 1813 664 603 579 327 303 212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1764 1796 6327
Size: 1764 32 4531
Fragments arranged by size:
4531 1764 32
PpuMI rG'GwC_Cy
Cuts at: 0 267 1478 3666 6081 6327
Size: 267 1211 2188 2415 246
Fragments arranged by size:
2415 2188 1211 267 246
PshAI GACnn'nnGTC
Cuts at: 0 979 1259 2032 6327
Size: 979 280 773 4295
Fragments arranged by size:
4295 979 773 280
PspOMI G'GGCC_C
Cuts at: 0 146 2229 3864 4458 4475 5692 6327
Size: 146 2083 1635 594 17 1217 635
Fragments arranged by size:
2083 1635 1217 635 594 146 17
PstI C_TGCA'G
Cuts at: 0 3125 3224 3767 5161 5763 6089 6327
Size: 3125 99 543 1394 602 326 238
Fragments arranged by size:
3125 1394 602 543 326 238 99
PvuII CAG'CTG
Cuts at: 0 2256 5184 5594 5826 6327
Size: 2256 2928 410 232 501
Fragments arranged by size:
2928 2256 501 410 232
SacI G_AGCT'C
Cuts at: 0 578 1355 3560 3960 6058 6327
Size: 578 777 2205 400 2098 269
Fragments arranged by size:
2205 2098 777 578 400 269
SalI G'TCGA_C
Cuts at: 0 2638 6327
Size: 2638 3689
SanDI GG'GwC_CC
Cuts at: 0 267 1478 6327
Size: 267 1211 4849
Fragments arranged by size:
4849 1211 267
SapI GCTCTTCn'nnn_
Cuts at: 0 836 3569 3948 5081 6184 6327
Size: 836 2733 379 1133 1103 143
Fragments arranged by size:
2733 1133 1103 836 379 143
SexAI A'CCwGG_T
Cuts at: 0 3790 6327
Size: 3790 2537
SfcI C'TryA_G
Cuts at: 0 205 2932 3121 3220 3763 4521 5157 5759
Size: 205 2727 189 99 543 758 636 602
Cuts at: 5759 6085 6327
Size: 326 242
Fragments arranged by size:
2727 758 636 602 543 326 242 205
189 99
SfoI GGC'GCC
Cuts at: 0 3070 4438 6327
Size: 3070 1368 1889
Fragments arranged by size:
3070 1889 1368
SmaI CCC'GGG
Cuts at: 0 164 5572 6327
Size: 164 5408 755
Fragments arranged by size:
5408 755 164
SmlI C'TyrA_G
Cuts at: 0 997 3379 6327
Size: 997 2382 2948
Fragments arranged by size:
2948 2382 997
SphI G_CATG'C
Cuts at: 0 3907 6327
Size: 3907 2420
SspI AAT'ATT
Cuts at: 0 3528 6327
Size: 3528 2799
StuI AGG'CCT
Cuts at: 0 2554 3985 4399 6039 6168 6327
Size: 2554 1431 414 1640 129 159
Fragments arranged by size:
2554 1640 1431 414 159 129
StyI C'CwwG_G
Cuts at: 0 908 1547 2084 2193 3242 3854 5225 5303
Size: 908 639 537 109 1049 612 1371 78
Cuts at: 5303 5414 5431 5688 6163 6236 6257 6327
Size: 111 17 257 475 73 21 70
Fragments arranged by size:
1371 1049 908 639 612 537 475 257
111 109 78 73 70 21 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 777 991 1544 1592 3331 3608 3626 5632
Size: 777 214 553 48 1739 277 18 2006
Cuts at: 5632 5647 6327
Size: 15 680
Fragments arranged by size:
2006 1739 777 680 553 277 214 48
18 15
TatI w'GTAC_w
Cuts at: 0 406 1280 2867 3284 4373 6327
Size: 406 874 1587 417 1089 1954
Fragments arranged by size:
1954 1587 1089 874 417 406
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3253 3285 3443 3475 4688 4720 6327
Size: 3253 32 158 32 1213 32 1607
Fragments arranged by size:
3253 1607 1213 158 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 28 6327
Size: 28 6299
XbaI T'CTAG_A
Cuts at: 0 2269 6327
Size: 2269 4058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 946 1368 1980 2454 3875 4662 6327
Size: 946 422 612 474 1421 787 1665
Fragments arranged by size:
1665 1421 946 787 612 474 422
XmaI C'CCGG_G
Cuts at: 0 162 5570 6327
Size: 162 5408 757
Fragments arranged by size:
5408 757 162
XmnI GAAnn'nnTTC
Cuts at: 0 2954 4399 6327
Size: 2954 1445 1928
Fragments arranged by size:
2954 1928 1445
ZraI GAC'GTC
Cuts at: 0 981 6327
Size: 981 5346
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI
PspOMI PstI PvuII SacI SalI SanDI SapI SexAI
SfcI SfoI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AflII AhdI AseI AsiSI AvrII BaeI BciVI
BfrBI BmgBI BsiWI BstBI BstEII BstZ17I BtgZI ClaI
CspCI EcoRV FspAI HpaI MluI NotI NruI NsiI
PacI PmeI PmlI PsiI PspXI PsrI PvuI RsrII
SacII SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SrfI SwaI XhoI