Restriction map for
pFN21AE3195
(Linear) (Six-base) MAPSORT of: KIEE3195.seq Check: 7741 from: 1 to: 1374
KIEE3195.orf
With 182 enzymes: SgfI *
March 22, 2012 16:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1291 1374
Size: 1291 83
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 1374
Size: 1175 199
AflIII A'CryG_T
Cuts at: 0 851 1374
Size: 851 523
AgeI A'CCGG_T
Cuts at: 0 1246 1374
Size: 1246 128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 260 1374
Size: 260 1114
ApaI G_GGCC'C
Cuts at: 0 43 1374
Size: 43 1331
ApaLI G'TGCA_C
Cuts at: 0 713 1374
Size: 713 661
ApoI r'AATT_y
Cuts at: 0 464 625 1374
Size: 464 161 749
Fragments arranged by size:
749 464 161
AvaI C'yCGr_G
Cuts at: 0 161 373 445 1374
Size: 161 212 72 929
Fragments arranged by size:
929 212 161 72
BamHI G'GATC_C
Cuts at: 0 419 893 1374
Size: 419 474 481
Fragments arranged by size:
481 474 419
BanII G_rGCy'C
Cuts at: 0 43 181 1370 1374
Size: 43 138 1189 4
Fragments arranged by size:
1189 138 43 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 1124 1163 1374
Size: 1124 39 211
Fragments arranged by size:
1124 211 39
Bme1580I G_kGCm'C
Cuts at: 0 43 717 1374
Size: 43 674 657
Fragments arranged by size:
674 657 43
BmgBI CAC'GTC
Cuts at: 0 429 1374
Size: 429 945
BmrI ACTGGGnnnn_n'
Cuts at: 0 112 1374
Size: 112 1262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 1374
Size: 521 853
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 1374
Size: 1339 35
BsaBI GATnn'nnATC
Cuts at: 0 418 1374
Size: 418 956
BsaWI w'CCGG_w
Cuts at: 0 476 1246 1374
Size: 476 770 128
Fragments arranged by size:
770 476 128
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 772 897 1374
Size: 265 507 125 477
Fragments arranged by size:
507 477 265 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1374
Size: 409 965
BsiEI CG_ry'CG
Cuts at: 0 25 131 318 1374
Size: 25 106 187 1056
Fragments arranged by size:
1056 187 106 25
BsiHKAI G_wGCw'C
Cuts at: 0 717 1370 1374
Size: 717 653 4
Fragments arranged by size:
717 653 4
Bsp1286I G_dGCh'C
Cuts at: 0 43 181 717 1370 1374
Size: 43 138 536 653 4
Fragments arranged by size:
653 536 138 43 4
BspEI T'CCGG_A
Cuts at: 0 476 1374
Size: 476 898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 1291 1374
Size: 353 938 83
Fragments arranged by size:
938 353 83
BsrDI GCAATG_nn'
Cuts at: 0 524 1374
Size: 524 850
BsrFI r'CCGG_y
Cuts at: 0 75 359 1246 1374
Size: 75 284 887 128
Fragments arranged by size:
887 284 128 75
BstYI r'GATC_y
Cuts at: 0 419 685 893 1002 1182 1374
Size: 419 266 208 109 180 192
Fragments arranged by size:
419 266 208 192 180 109
BtgI C'CryG_G
Cuts at: 0 1289 1374
Size: 1289 85
EaeI y'GGCC_r
Cuts at: 0 22 128 263 548 867 1241 1374
Size: 22 106 135 285 319 374 133
Fragments arranged by size:
374 319 285 135 133 106 22
EagI C'GGCC_G
Cuts at: 0 22 128 1374
Size: 22 106 1246
Fragments arranged by size:
1246 106 22
EcoICRI GAG'CTC
Cuts at: 0 1368 1374
Size: 1368 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 1175 1374
Size: 521 654 199
Fragments arranged by size:
654 521 199
EcoO109I rG'GnC_Cy
Cuts at: 0 39 777 1218 1374
Size: 39 738 441 156
Fragments arranged by size:
738 441 156 39
EcoRI G'AATT_C
Cuts at: 0 625 1374
Size: 625 749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 693 1374
Size: 693 681
HincII GTy'rAC
Cuts at: 0 756 1374
Size: 756 618
MfeI C'AATT_G
Cuts at: 0 506 1374
Size: 506 868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 1374
Size: 713 661
MscI TGG'CCA
Cuts at: 0 550 869 1374
Size: 550 319 505
Fragments arranged by size:
550 505 319
MslI CAynn'nnrTG
Cuts at: 0 431 1374
Size: 431 943
MspA1I CmG'CkG
Cuts at: 0 27 546 702 1344 1374
Size: 27 519 156 642 30
Fragments arranged by size:
642 519 156 30 27
NotI GC'GGCC_GC
Cuts at: 0 22 128 1374
Size: 22 106 1246
Fragments arranged by size:
1246 106 22
NspI r_CATG'y
Cuts at: 0 188 324 677 855 1374
Size: 188 136 353 178 519
Fragments arranged by size:
519 353 188 178 136
PasI CC'CwG_GG
Cuts at: 0 43 1374
Size: 43 1331
PciI A'CATG_T
Cuts at: 0 851 1374
Size: 851 523
PfoI T'CCnGG_A
Cuts at: 0 725 1214 1374
Size: 725 489 160
Fragments arranged by size:
725 489 160
PpuMI rG'GwC_Cy
Cuts at: 0 777 1218 1374
Size: 777 441 156
Fragments arranged by size:
777 441 156
PspOMI G'GGCC_C
Cuts at: 0 39 1374
Size: 39 1335
PstI C_TGCA'G
Cuts at: 0 350 943 1374
Size: 350 593 431
Fragments arranged by size:
593 431 350
PvuII CAG'CTG
Cuts at: 0 702 1374
Size: 702 672
SacI G_AGCT'C
Cuts at: 0 1370 1374
Size: 1370 4
ScaI AGT'ACT
Cuts at: 0 525 1374
Size: 525 849
SfcI C'TryA_G
Cuts at: 0 12 346 939 1374
Size: 12 334 593 435
Fragments arranged by size:
593 435 334 12
SgrAI Cr'CCGG_yG
Cuts at: 0 75 1246 1374
Size: 75 1171 128
Fragments arranged by size:
1171 128 75
SmlI C'TyrA_G
Cuts at: 0 1354 1374
Size: 1354 20
SphI G_CATG'C
Cuts at: 0 188 324 677 1374
Size: 188 136 353 697
Fragments arranged by size:
697 353 188 136
SspI AAT'ATT
Cuts at: 0 616 1374
Size: 616 758
StyI C'CwwG_G
Cuts at: 0 266 1374
Size: 266 1108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 780 1374
Size: 780 594
TatI w'GTAC_w
Cuts at: 0 523 1374
Size: 523 851
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 707 739 1374
Size: 707 32 635
Fragments arranged by size:
707 635 32
Tth111I GACn'n_nGTC
Cuts at: 0 742 1374
Size: 742 632
Enzymes that do cut:
AarI AcuI AflIII AgeI AlfI ApaI ApaLI ApoI
AvaI BamHI BanII BbsI Bme1580I BmgBI BmrI BpmI
BpuEI BsaBI BsaWI BseRI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrDI BsrFI BstYI BtgI EaeI EagI
EcoICRI Eco57MI EcoO109I EcoRI FalI HincII MfeI MmeI
MscI MslI MspA1I NotI NspI PasI PciI PfoI
PpuMI PspOMI PstI PvuII SacI ScaI SfcI SgrAI
SmlI SphI SspI StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AleI
AloI AlwNI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaXI
BseYI BsiWI BsmI BsmBI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoNI EcoRV FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI KpnI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI PacI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SmaI SnaBI SpeI SrfI StuI SwaI XbaI XcmI
XhoI XmaI XmnI ZraI