Restriction map for
pFN21AE3192
(Linear) (Six-base) MAPSORT of: KIEE3192.seq Check: 2802 from: 1 to: 1341
KIEE3192.orf
With 182 enzymes: SgfI *
March 22, 2012 16:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1341
Size: 962 379
AflIII A'CryG_T
Cuts at: 0 752 1341
Size: 752 589
AlwNI CAG_nnn'CTG
Cuts at: 0 40 731 1341
Size: 40 691 610
Fragments arranged by size:
691 610 40
AvaI C'yCGr_G
Cuts at: 0 281 1341
Size: 281 1060
BanI G'GyrC_C
Cuts at: 0 872 1341
Size: 872 469
BanII G_rGCy'C
Cuts at: 0 144 327 1341
Size: 144 183 1014
Fragments arranged by size:
1014 183 144
BbvCI CC'TCA_GC
Cuts at: 0 41 1341
Size: 41 1300
BciVI GTATCCnnnnn_n'
Cuts at: 0 1199 1341
Size: 1199 142
BglII A'GATC_T
Cuts at: 0 111 471 1341
Size: 111 360 870
Fragments arranged by size:
870 360 111
BmgBI CAC'GTC
Cuts at: 0 755 1341
Size: 755 586
BmrI ACTGGGnnnn_n'
Cuts at: 0 1015 1341
Size: 1015 326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 238 1341
Size: 238 1103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 149 208 392 780 1341
Size: 112 37 59 184 388 561
Fragments arranged by size:
561 388 184 112 59 37
Bpu10I CC'TnA_GC
Cuts at: 0 41 536 1341
Size: 41 495 805
Fragments arranged by size:
805 495 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 1341
Size: 562 779
BsaI GGTCTCn'nnnn_
Cuts at: 0 410 677 1341
Size: 410 267 664
Fragments arranged by size:
664 410 267
BsaBI GATnn'nnATC
Cuts at: 0 592 1341
Size: 592 749
BsaHI Gr'CG_yC
Cuts at: 0 699 1341
Size: 699 642
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 390 420 631 661 1341
Size: 390 30 211 30 680
Fragments arranged by size:
680 390 211 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 1341
Size: 678 663
BsiHKAI G_wGCw'C
Cuts at: 0 144 327 1341
Size: 144 183 1014
Fragments arranged by size:
1014 183 144
Bsp1286I G_dGCh'C
Cuts at: 0 144 327 1341
Size: 144 183 1014
Fragments arranged by size:
1014 183 144
BsrGI T'GTAC_A
Cuts at: 0 590 1125 1280 1341
Size: 590 535 155 61
Fragments arranged by size:
590 535 155 61
BssSI C'ACGA_G
Cuts at: 0 785 1341
Size: 785 556
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1258 1341
Size: 1258 83
BstEII G'GTnAC_C
Cuts at: 0 1177 1341
Size: 1177 164
BstYI r'GATC_y
Cuts at: 0 111 471 1341
Size: 111 360 870
Fragments arranged by size:
870 360 111
Bsu36I CC'TnA_GG
Cuts at: 0 29 1341
Size: 29 1312
DraIII CAC_nnn'GTG
Cuts at: 0 879 1341
Size: 879 462
EaeI y'GGCC_r
Cuts at: 0 915 1341
Size: 915 426
EarI CTCTTCn'nnn_
Cuts at: 0 295 938 1341
Size: 295 643 403
Fragments arranged by size:
643 403 295
EcoICRI GAG'CTC
Cuts at: 0 142 325 1341
Size: 142 183 1016
Fragments arranged by size:
1016 183 142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 149 208 392 780 962 1341
Size: 112 37 59 184 388 182 379
Fragments arranged by size:
388 379 184 182 112 59 37
EcoO109I rG'GnC_Cy
Cuts at: 0 582 1341
Size: 582 759
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 616 631 648 656 663 688
Size: 463 32 121 15 17 8 7 25
Cuts at: 688 937 969 1341
Size: 249 32 372
Fragments arranged by size:
463 372 249 121 32 32 25 17
15 8 7
HincII GTy'rAC
Cuts at: 0 932 1341
Size: 932 409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1341
Size: 405 936
MscI TGG'CCA
Cuts at: 0 917 1341
Size: 917 424
MslI CAynn'nnrTG
Cuts at: 0 565 1341
Size: 565 776
PfoI T'CCnGG_A
Cuts at: 0 757 1341
Size: 757 584
PpuMI rG'GwC_Cy
Cuts at: 0 582 1341
Size: 582 759
PshAI GACnn'nnGTC
Cuts at: 0 1252 1341
Size: 1252 89
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 966 998 1341
Size: 966 32 343
Fragments arranged by size:
966 343 32
PstI C_TGCA'G
Cuts at: 0 119 1254 1341
Size: 119 1135 87
Fragments arranged by size:
1135 119 87
SacI G_AGCT'C
Cuts at: 0 144 327 1341
Size: 144 183 1014
Fragments arranged by size:
1014 183 144
SapI GCTCTTCn'nnn_
Cuts at: 0 938 1341
Size: 938 403
SfcI C'TryA_G
Cuts at: 0 115 861 1159 1250 1341
Size: 115 746 298 91 91
Fragments arranged by size:
746 298 115 91 91
SmlI C'TyrA_G
Cuts at: 0 281 541 1341
Size: 281 260 800
Fragments arranged by size:
800 281 260
SspI AAT'ATT
Cuts at: 0 886 1341
Size: 886 455
StuI AGG'CCT
Cuts at: 0 1335 1341
Size: 1335 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 594 1341
Size: 594 747
TatI w'GTAC_w
Cuts at: 0 590 1125 1280 1341
Size: 590 535 155 61
Fragments arranged by size:
590 535 155 61
XhoI C'TCGA_G
Cuts at: 0 281 1341
Size: 281 1060
Enzymes that do cut:
AcuI AflIII AlwNI AvaI BanI BanII BbvCI BciVI
BglII BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BsgI BsiHKAI Bsp1286I BsrGI BssSI
BstAPI BstEII BstYI Bsu36I DraIII EaeI EarI EcoICRI
Eco57MI EcoO109I Hin4I HincII MmeI MscI MslI PfoI
PpuMI PshAI PsrI PstI SacI SapI SfcI SmlI
SspI StuI TaqII TatI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI
BclI BfrBI BglI BlpI Bme1580I BmtI BsaAI BsaWI
BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BstBI BstXI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PsiI PspOMI PspXI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StyI SwaI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI