Restriction map for
pFN21AE3189
(Linear) (Six-base) MAPSORT of: KIEE3189.seq Check: 2036 from: 1 to: 1296
KIEE3189.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AfeI AGC'GCT
Cuts at: 0 136 166 322 1296
Size: 136 30 156 974
Fragments arranged by size:
974 156 136 30
AflIII A'CryG_T
Cuts at: 0 284 1296
Size: 284 1012
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 322 824 1296
Size: 322 502 472
Fragments arranged by size:
502 472 322
AlwNI CAG_nnn'CTG
Cuts at: 0 1074 1296
Size: 1074 222
ApaLI G'TGCA_C
Cuts at: 0 1288 1296
Size: 1288 8
BanI G'GyrC_C
Cuts at: 0 385 401 1089 1176 1296
Size: 385 16 688 87 120
Fragments arranged by size:
688 385 120 87 16
BanII G_rGCy'C
Cuts at: 0 22 751 1145 1235 1296
Size: 22 729 394 90 61
Fragments arranged by size:
729 394 90 61 22
BbeI G_GCGC'C
Cuts at: 0 389 1180 1296
Size: 389 791 116
Fragments arranged by size:
791 389 116
BbvCI CC'TCA_GC
Cuts at: 0 623 1296
Size: 623 673
BglI GCCn_nnn'nGGC
Cuts at: 0 670 772 917 979 1296
Size: 670 102 145 62 317
Fragments arranged by size:
670 317 145 102 62
BlpI GC'TnA_GC
Cuts at: 0 106 1296
Size: 106 1190
Bme1580I G_kGCm'C
Cuts at: 0 404 908 1094 1292 1296
Size: 404 504 186 198 4
Fragments arranged by size:
504 404 198 186 4
BmgBI CAC'GTC
Cuts at: 0 788 1296
Size: 788 508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1249 1296
Size: 878 371 47
Fragments arranged by size:
878 371 47
Bpu10I CC'TnA_GC
Cuts at: 0 623 1296
Size: 623 673
BsaHI Gr'CG_yC
Cuts at: 0 312 386 717 781 874 953 1177 1296
Size: 312 74 331 64 93 79 224 119
Fragments arranged by size:
331 312 224 119 93 79 74 64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 178 208 992 1022 1296
Size: 178 30 784 30 274
Fragments arranged by size:
784 274 178 30 30
BseYI C'CCAG_C
Cuts at: 0 488 608 744 1296
Size: 488 120 136 552
Fragments arranged by size:
552 488 136 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 1296
Size: 250 1046
BsiEI CG_ry'CG
Cuts at: 0 10 89 665 689 755 776 983 1202
Size: 10 79 576 24 66 21 207 219
Cuts at: 1202 1253 1296
Size: 51 43
Fragments arranged by size:
576 219 207 79 66 51 43 24
21 10
BsiHKAI G_wGCw'C
Cuts at: 0 22 158 1195 1292 1296
Size: 22 136 1037 97 4
Fragments arranged by size:
1037 136 97 22 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 638 1296
Size: 638 658
Bsp1286I G_dGCh'C
Cuts at: 0 22 158 404 751 908 1094 1145 1195
Size: 22 136 246 347 157 186 51 50
Cuts at: 1195 1235 1292 1296
Size: 40 57 4
Fragments arranged by size:
347 246 186 157 136 57 51 50
40 22 4
BsrFI r'CCGG_y
Cuts at: 0 388 1086 1296
Size: 388 698 210
Fragments arranged by size:
698 388 210
BssHII G'CGCG_C
Cuts at: 0 336 377 1296
Size: 336 41 919
Fragments arranged by size:
919 336 41
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1281 1296
Size: 1281 15
BtgI C'CryG_G
Cuts at: 0 4 68 223 665 689 698 770 776
Size: 4 64 155 442 24 9 72 6
Cuts at: 776 977 1154 1196 1202 1296
Size: 201 177 42 6 94
Fragments arranged by size:
442 201 177 155 94 72 64 42
24 9 6 6 4
DraIII CAC_nnn'GTG
Cuts at: 0 1190 1281 1296
Size: 1190 91 15
Fragments arranged by size:
1190 91 15
EaeI y'GGCC_r
Cuts at: 0 7 86 662 686 752 773 828 909
Size: 7 79 576 24 66 21 55 81
Cuts at: 909 940 968 980 1199 1250 1296
Size: 31 28 12 219 51 46
Fragments arranged by size:
576 219 81 79 66 55 51 46
31 28 24 21 12 7
EagI C'GGCC_G
Cuts at: 0 7 86 662 686 752 773 980 1199
Size: 7 79 576 24 66 21 207 219
Cuts at: 1199 1250 1296
Size: 51 46
Fragments arranged by size:
576 219 207 79 66 51 46 24
21 7
EarI CTCTTCn'nnn_
Cuts at: 0 10 1296
Size: 10 1286
EcoICRI GAG'CTC
Cuts at: 0 20 1296
Size: 20 1276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1249 1296
Size: 878 371 47
Fragments arranged by size:
878 371 47
EcoO109I rG'GnC_Cy
Cuts at: 0 1025 1035 1296
Size: 1025 10 261
Fragments arranged by size:
1025 261 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 270 822 1296
Size: 270 552 474
Fragments arranged by size:
552 474 270
HaeII r_GCGC'y
Cuts at: 0 138 168 265 324 389 1180 1296
Size: 138 30 97 59 65 791 116
Fragments arranged by size:
791 138 116 97 65 59 30
KasI G'GCGC_C
Cuts at: 0 385 1176 1296
Size: 385 791 120
Fragments arranged by size:
791 385 120
MslI CAynn'nnrTG
Cuts at: 0 1087 1296
Size: 1087 209
MspA1I CmG'CkG
Cuts at: 0 6 105 225 691 700 763 772 778
Size: 6 99 120 466 9 63 9 6
Cuts at: 778 979 1156 1198 1204 1296
Size: 201 177 42 6 92
Fragments arranged by size:
466 201 177 120 99 92 63 42
9 9 6 6 6
NarI GG'CG_CC
Cuts at: 0 386 1177 1296
Size: 386 791 119
Fragments arranged by size:
791 386 119
NcoI C'CATG_G
Cuts at: 0 68 1296
Size: 68 1228
NotI GC'GGCC_GC
Cuts at: 0 686 773 980 1199 1296
Size: 686 87 207 219 97
Fragments arranged by size:
686 219 207 97 87
NspI r_CATG'y
Cuts at: 0 288 1296
Size: 288 1008
PciI A'CATG_T
Cuts at: 0 284 1296
Size: 284 1012
PfoI T'CCnGG_A
Cuts at: 0 393 1296
Size: 393 903
PpuMI rG'GwC_Cy
Cuts at: 0 1035 1296
Size: 1035 261
PshAI GACnn'nnGTC
Cuts at: 0 720 1296
Size: 720 576
PstI C_TGCA'G
Cuts at: 0 854 1136 1296
Size: 854 282 160
Fragments arranged by size:
854 282 160
RsrII CG'GwC_CG
Cuts at: 0 391 1296
Size: 391 905
SacI G_AGCT'C
Cuts at: 0 22 1296
Size: 22 1274
SacII CC_GC'GG
Cuts at: 0 7 226 692 701 773 779 980 1157
Size: 7 219 466 9 72 6 201 177
Cuts at: 1157 1199 1205 1296
Size: 42 6 91
Fragments arranged by size:
466 219 201 177 91 72 42 9
7 6 6
SanDI GG'GwC_CC
Cuts at: 0 1035 1296
Size: 1035 261
SapI GCTCTTCn'nnn_
Cuts at: 0 10 1296
Size: 10 1286
SfcI C'TryA_G
Cuts at: 0 850 1007 1132 1296
Size: 850 157 125 164
Fragments arranged by size:
850 164 157 125
SfoI GGC'GCC
Cuts at: 0 387 1178 1296
Size: 387 791 118
Fragments arranged by size:
791 387 118
StyI C'CwwG_G
Cuts at: 0 68 869 1296
Size: 68 801 427
Fragments arranged by size:
801 427 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 497 1296
Size: 497 799
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 178 210 1296
Size: 178 32 1086
Fragments arranged by size:
1086 178 32
Enzymes that do cut:
AfeI AflIII AlfI AlwNI ApaLI BanI BanII BbeI
BbvCI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaHI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI
BssHII BstAPI BtgI DraIII EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FalI HaeII KasI MslI MspA1I NarI
NcoI NotI NspI PciI PfoI PpuMI PshAI PstI
RsrII SacI SacII SanDI SapI SfcI SfoI StyI
TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI AloI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BglII BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BseRI BsiWI BsmI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NaeI NdeI NgoMIV NheI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI PvuII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI