Restriction map for
pFN21AE3188
(Linear) (Six-base) MAPSORT of: KIEE3188.seq Check: 8321 from: 1 to: 1296
KIEE3188.orf
With 182 enzymes: SgfI *
March 22, 2012 16:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 221 1296
Size: 221 1075
AccI GT'mk_AC
Cuts at: 0 437 1296
Size: 437 859
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1296
Size: 683 613
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 488 520 1296
Size: 488 32 776
Fragments arranged by size:
776 488 32
AlwNI CAG_nnn'CTG
Cuts at: 0 78 119 240 411 481 844 921 971
Size: 78 41 121 171 70 363 77 50
Cuts at: 971 1017 1296
Size: 46 279
Fragments arranged by size:
363 279 171 121 78 77 70 50
46 41
ApoI r'AATT_y
Cuts at: 0 617 1296
Size: 617 679
AvaI C'yCGr_G
Cuts at: 0 309 1296
Size: 309 987
BanI G'GyrC_C
Cuts at: 0 148 193 350 1296
Size: 148 45 157 946
Fragments arranged by size:
946 157 148 45
BanII G_rGCy'C
Cuts at: 0 22 286 316 1296
Size: 22 264 30 980
Fragments arranged by size:
980 264 30 22
BbeI G_GCGC'C
Cuts at: 0 152 197 354 1296
Size: 152 45 157 942
Fragments arranged by size:
942 157 152 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 692 917 1296
Size: 692 225 379
Fragments arranged by size:
692 379 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 559 593 1296
Size: 559 34 703
Fragments arranged by size:
703 559 34
BglI GCCn_nnn'nGGC
Cuts at: 0 191 1296
Size: 191 1105
BmrI ACTGGGnnnn_n'
Cuts at: 0 951 981 1296
Size: 951 30 315
Fragments arranged by size:
951 315 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 616 770 1296
Size: 616 154 526
Fragments arranged by size:
616 526 154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 300 312 835 894 1296
Size: 154 146 12 523 59 402
Fragments arranged by size:
523 402 154 146 59 12
Bpu10I CC'TnA_GC
Cuts at: 0 404 1296
Size: 404 892
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1296
Size: 1165 131
BsaHI Gr'CG_yC
Cuts at: 0 149 158 194 351 1296
Size: 149 9 36 157 945
Fragments arranged by size:
945 157 149 36 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 1296
Size: 127 30 1139
Fragments arranged by size:
1139 127 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 696 1162 1209 1296
Size: 696 466 47 87
Fragments arranged by size:
696 466 87 47
BseYI C'CCAG_C
Cuts at: 0 167 1296
Size: 167 1129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 1296
Size: 722 574
BsiEI CG_ry'CG
Cuts at: 0 338 1296
Size: 338 958
Bsp1286I G_dGCh'C
Cuts at: 0 22 286 316 1296
Size: 22 264 30 980
Fragments arranged by size:
980 264 30 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 221 1296
Size: 221 1075
BsrDI GCAATG_nn'
Cuts at: 0 715 1296
Size: 715 581
BstAPI GCAn_nnn'nTGC
Cuts at: 0 68 1296
Size: 68 1228
BstYI r'GATC_y
Cuts at: 0 534 1296
Size: 534 762
BtgI C'CryG_G
Cuts at: 0 542 1296
Size: 542 754
BtsI GCAGTG_nn'
Cuts at: 0 126 297 1296
Size: 126 171 999
Fragments arranged by size:
999 171 126
EaeI y'GGCC_r
Cuts at: 0 41 108 242 335 1296
Size: 41 67 134 93 961
Fragments arranged by size:
961 134 93 67 41
EagI C'GGCC_G
Cuts at: 0 335 1296
Size: 335 961
EarI CTCTTCn'nnn_
Cuts at: 0 379 790 1296
Size: 379 411 506
Fragments arranged by size:
506 411 379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 12 1296
Size: 12 1284
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 300 312 683 835 894 1296
Size: 154 146 12 371 152 59 402
Fragments arranged by size:
402 371 154 152 146 59 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 511 1147 1296
Size: 511 636 149
Fragments arranged by size:
636 511 149
EcoO109I rG'GnC_Cy
Cuts at: 0 138 237 344 467 918 1014 1296
Size: 138 99 107 123 451 96 282
Fragments arranged by size:
451 282 138 123 107 99 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1179 1296
Size: 1179 117
HaeII r_GCGC'y
Cuts at: 0 152 197 354 1296
Size: 152 45 157 942
Fragments arranged by size:
942 157 152 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 834 866 1296
Size: 834 32 430
Fragments arranged by size:
834 430 32
HindIII A'AGCT_T
Cuts at: 0 1156 1189 1296
Size: 1156 33 107
Fragments arranged by size:
1156 107 33
KasI G'GCGC_C
Cuts at: 0 148 193 350 1296
Size: 148 45 157 946
Fragments arranged by size:
946 157 148 45
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 593 805 1296
Size: 311 282 212 491
Fragments arranged by size:
491 311 282 212
MspA1I CmG'CkG
Cuts at: 0 167 369 577 1296
Size: 167 202 208 719
Fragments arranged by size:
719 208 202 167
NarI GG'CG_CC
Cuts at: 0 149 194 351 1296
Size: 149 45 157 945
Fragments arranged by size:
945 157 149 45
NcoI C'CATG_G
Cuts at: 0 542 1296
Size: 542 754
NotI GC'GGCC_GC
Cuts at: 0 335 1296
Size: 335 961
PflMI CCAn_nnn'nTGG
Cuts at: 0 132 1017 1296
Size: 132 885 279
Fragments arranged by size:
885 279 132
PfoI T'CCnGG_A
Cuts at: 0 686 1260 1296
Size: 686 574 36
Fragments arranged by size:
686 574 36
PpuMI rG'GwC_Cy
Cuts at: 0 237 1014 1296
Size: 237 777 282
Fragments arranged by size:
777 282 237
PstI C_TGCA'G
Cuts at: 0 236 368 458 582 811 1296
Size: 236 132 90 124 229 485
Fragments arranged by size:
485 236 229 132 124 90
PvuII CAG'CTG
Cuts at: 0 577 1296
Size: 577 719
SapI GCTCTTCn'nnn_
Cuts at: 0 379 790 1296
Size: 379 411 506
Fragments arranged by size:
506 411 379
SexAI A'CCwGG_T
Cuts at: 0 1010 1296
Size: 1010 286
SfcI C'TryA_G
Cuts at: 0 232 364 454 578 807 1296
Size: 232 132 90 124 229 489
Fragments arranged by size:
489 232 229 132 124 90
SfoI GGC'GCC
Cuts at: 0 150 195 352 1296
Size: 150 45 157 944
Fragments arranged by size:
944 157 150 45
SmaI CCC'GGG
Cuts at: 0 311 1296
Size: 311 985
SmlI C'TyrA_G
Cuts at: 0 1144 1296
Size: 1144 152
SrfI GCCC'GGGC
Cuts at: 0 311 1296
Size: 311 985
StyI C'CwwG_G
Cuts at: 0 542 824 1101 1296
Size: 542 282 277 195
Fragments arranged by size:
542 282 277 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 127 159 1296
Size: 127 32 1137
Fragments arranged by size:
1137 127 32
Tth111I GACn'n_nGTC
Cuts at: 0 442 846 1012 1296
Size: 442 404 166 284
Fragments arranged by size:
442 404 284 166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 976 1296
Size: 976 320
XmaI C'CCGG_G
Cuts at: 0 309 1296
Size: 309 987
XmnI GAAnn'nnTTC
Cuts at: 0 617 1296
Size: 617 679
Enzymes that do cut:
AarI AccI AcuI AloI AlwNI ApoI AvaI BanI
BanII BbeI BbsI BcgI BglI BmrI BplI BpmI
Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI
Bsp1286I BspMI BsrDI BstAPI BstYI BtgI BtsI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HindIII KasI MmeI MspA1I NarI NcoI NotI
PflMI PfoI PpuMI PstI PvuII SapI SexAI SfcI
SfoI SmaI SmlI SrfI StyI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmtI BsaI BsaAI BsaBI BsaWI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
MscI MslI NaeI NdeI NgoMIV NheI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI TaqII TatI XbaI
XhoI ZraI