Restriction map for
pFN21AE3187
(Linear) (Six-base) MAPSORT of: KIEE3187.seq Check: 539 from: 1 to: 1284
KIEE3187.orf
With 182 enzymes: SgfI *
March 22, 2012 16:19 ..
AccI GT'mk_AC
Cuts at: 0 491 1161 1284
Size: 491 670 123
Fragments arranged by size:
670 491 123
Acc65I G'GTAC_C
Cuts at: 0 252 1284
Size: 252 1032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1085 1284
Size: 137 948 199
Fragments arranged by size:
948 199 137
AflIII A'CryG_T
Cuts at: 0 143 904 1137 1284
Size: 143 761 233 147
Fragments arranged by size:
761 233 147 143
AleI CACnn'nnGTG
Cuts at: 0 169 482 1284
Size: 169 313 802
Fragments arranged by size:
802 313 169
ApaI G_GGCC'C
Cuts at: 0 269 1284
Size: 269 1015
ApoI r'AATT_y
Cuts at: 0 300 533 1185 1284
Size: 300 233 652 99
Fragments arranged by size:
652 300 233 99
AvaI C'yCGr_G
Cuts at: 0 107 268 445 480 1284
Size: 107 161 177 35 804
Fragments arranged by size:
804 177 161 107 35
BanI G'GyrC_C
Cuts at: 0 96 252 309 1284
Size: 96 156 57 975
Fragments arranged by size:
975 156 96 57
BanII G_rGCy'C
Cuts at: 0 269 717 1284
Size: 269 448 567
Fragments arranged by size:
567 448 269
BbsI GAAGACnn'nnnn_
Cuts at: 0 1244 1284
Size: 1244 40
BciVI GTATCCnnnnn_n'
Cuts at: 0 1035 1284
Size: 1035 249
BglI GCCn_nnn'nGGC
Cuts at: 0 38 264 1284
Size: 38 226 1020
Fragments arranged by size:
1020 226 38
BlpI GC'TnA_GC
Cuts at: 0 193 1284
Size: 193 1091
Bme1580I G_kGCm'C
Cuts at: 0 269 314 1284
Size: 269 45 970
Fragments arranged by size:
970 269 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 443 1150 1284
Size: 250 193 707 134
Fragments arranged by size:
707 250 193 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 30 1072 1284
Size: 30 1042 212
Fragments arranged by size:
1042 212 30
BsaAI yAC'GTr
Cuts at: 0 1140 1284
Size: 1140 144
BsaHI Gr'CG_yC
Cuts at: 0 418 1284
Size: 418 866
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 1284
Size: 193 30 1061
Fragments arranged by size:
1061 193 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 1041 1284
Size: 214 827 243
Fragments arranged by size:
827 243 214
BseYI C'CCAG_C
Cuts at: 0 241 1284
Size: 241 1043
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 506 1162 1284
Size: 360 146 656 122
Fragments arranged by size:
656 360 146 122
BsiHKAI G_wGCw'C
Cuts at: 0 717 1284
Size: 717 567
BsiWI C'GTAC_G
Cuts at: 0 178 1284
Size: 178 1106
BsmI GAATG_Cn'
Cuts at: 0 711 1284
Size: 711 573
Bsp1286I G_dGCh'C
Cuts at: 0 269 314 717 1284
Size: 269 45 403 567
Fragments arranged by size:
567 403 269 45
BspHI T'CATG_A
Cuts at: 0 506 1284
Size: 506 778
BsrDI GCAATG_nn'
Cuts at: 0 462 1284
Size: 462 822
BstXI CCAn_nnnn'nTGG
Cuts at: 0 60 1284
Size: 60 1224
BstYI r'GATC_y
Cuts at: 0 392 1284
Size: 392 892
BtgI C'CryG_G
Cuts at: 0 33 314 916 1284
Size: 33 281 602 368
Fragments arranged by size:
602 368 281 33
BtsI GCAGTG_nn'
Cuts at: 0 128 1202 1284
Size: 128 1074 82
Fragments arranged by size:
1074 128 82
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1172 1207 1284
Size: 1172 35 77
Fragments arranged by size:
1172 77 35
EaeI y'GGCC_r
Cuts at: 0 213 1284
Size: 213 1071
EarI CTCTTCn'nnn_
Cuts at: 0 1055 1284
Size: 1055 229
EcoICRI GAG'CTC
Cuts at: 0 715 1284
Size: 715 569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 250 443 1085 1150 1284
Size: 137 113 193 642 65 134
Fragments arranged by size:
642 193 137 134 113 65
EcoO109I rG'GnC_Cy
Cuts at: 0 246 449 1284
Size: 246 203 835
Fragments arranged by size:
835 246 203
HaeII r_GCGC'y
Cuts at: 0 277 1284
Size: 277 1007
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 416 448 501 533 1069 1101 1284
Size: 416 32 53 32 536 32 183
Fragments arranged by size:
536 416 183 53 32 32 32
HincII GTy'rAC
Cuts at: 0 912 1148 1218 1284
Size: 912 236 70 66
Fragments arranged by size:
912 236 70 66
HindIII A'AGCT_T
Cuts at: 0 968 1284
Size: 968 316
KpnI G_GTAC'C
Cuts at: 0 256 1284
Size: 256 1028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1284
Size: 384 900
MslI CAynn'nnrTG
Cuts at: 0 169 469 482 562 1284
Size: 169 300 13 80 722
Fragments arranged by size:
722 300 169 80 13
MspA1I CmG'CkG
Cuts at: 0 35 228 1167 1284
Size: 35 193 939 117
Fragments arranged by size:
939 193 117 35
NcoI C'CATG_G
Cuts at: 0 916 1284
Size: 916 368
NspI r_CATG'y
Cuts at: 0 908 1284
Size: 908 376
PciI A'CATG_T
Cuts at: 0 904 1284
Size: 904 380
PflMI CCAn_nnn'nTGG
Cuts at: 0 320 1284
Size: 320 964
PmlI CAC'GTG
Cuts at: 0 1140 1284
Size: 1140 144
PpuMI rG'GwC_Cy
Cuts at: 0 246 449 1284
Size: 246 203 835
Fragments arranged by size:
835 246 203
PshAI GACnn'nnGTC
Cuts at: 0 571 1284
Size: 571 713
PspOMI G'GGCC_C
Cuts at: 0 265 1284
Size: 265 1019
PvuII CAG'CTG
Cuts at: 0 1167 1284
Size: 1167 117
SacI G_AGCT'C
Cuts at: 0 717 1284
Size: 717 567
SacII CC_GC'GG
Cuts at: 0 36 1284
Size: 36 1248
SanDI GG'GwC_CC
Cuts at: 0 449 1284
Size: 449 835
SfcI C'TryA_G
Cuts at: 0 1162 1284
Size: 1162 122
SmaI CCC'GGG
Cuts at: 0 109 270 447 482 1284
Size: 109 161 177 35 802
Fragments arranged by size:
802 177 161 109 35
StyI C'CwwG_G
Cuts at: 0 601 916 1284
Size: 601 315 368
Fragments arranged by size:
601 368 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 594 1284
Size: 594 690
TatI w'GTAC_w
Cuts at: 0 1104 1284
Size: 1104 180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 317 349 968 1000 1284
Size: 317 32 619 32 284
Fragments arranged by size:
619 317 284 32 32
XmaI C'CCGG_G
Cuts at: 0 107 268 445 480 1284
Size: 107 161 177 35 804
Fragments arranged by size:
804 177 161 107 35
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI ApaI ApoI AvaI
BanI BanII BbsI BciVI BglI BlpI Bme1580I BpmI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsiWI BsmI Bsp1286I BspHI BsrDI BstXI BstYI BtgI
BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I HincII HindIII KpnI MmeI MslI MspA1I NcoI
NspI PciI PflMI PmlI PpuMI PshAI PspOMI PvuII
SacI SacII SanDI SfcI SmaI StyI TaqII TatI
TstI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BclI BfrBI BglII BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsiEI
BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KasI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PpiI PsiI PspXI PsrI
PstI PvuI RsrII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmnI ZraI