Restriction map for
pFN21AE3181
(Linear) (Six-base) MAPSORT of: KIEE3181.seq Check: 304 from: 1 to: 1254
KIEE3181.orf
With 182 enzymes: SgfI *
March 22, 2012 16:18 ..
AccI GT'mk_AC
Cuts at: 0 313 1254
Size: 313 941
ApaI G_GGCC'C
Cuts at: 0 252 1254
Size: 252 1002
ApaLI G'TGCA_C
Cuts at: 0 1193 1254
Size: 1193 61
AvaI C'yCGr_G
Cuts at: 0 245 1254
Size: 245 1009
BanII G_rGCy'C
Cuts at: 0 252 1254
Size: 252 1002
BbsI GAAGACnn'nnnn_
Cuts at: 0 526 602 1254
Size: 526 76 652
Fragments arranged by size:
652 526 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 824 858 1254
Size: 824 34 396
Fragments arranged by size:
824 396 34
BclI T'GATC_A
Cuts at: 0 12 1254
Size: 12 1242
BfrBI ATG'CAT
Cuts at: 0 573 1254
Size: 573 681
BlpI GC'TnA_GC
Cuts at: 0 671 1254
Size: 671 583
Bme1580I G_kGCm'C
Cuts at: 0 252 847 1197 1254
Size: 252 595 350 57
Fragments arranged by size:
595 350 252 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 966 1254
Size: 966 288
BsaI GGTCTCn'nnnn_
Cuts at: 0 86 1254
Size: 86 1168
BsaAI yAC'GTr
Cuts at: 0 655 1254
Size: 655 599
BsaHI Gr'CG_yC
Cuts at: 0 1067 1254
Size: 1067 187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 99 129 1254
Size: 99 30 1125
Fragments arranged by size:
1125 99 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 176 1254
Size: 176 1078
BseYI C'CCAG_C
Cuts at: 0 160 1254
Size: 160 1094
BsiEI CG_ry'CG
Cuts at: 0 313 1254
Size: 313 941
BsiHKAI G_wGCw'C
Cuts at: 0 1197 1254
Size: 1197 57
Bsp1286I G_dGCh'C
Cuts at: 0 252 847 1197 1254
Size: 252 595 350 57
Fragments arranged by size:
595 350 252 57
BspHI T'CATG_A
Cuts at: 0 276 1254
Size: 276 978
BsrDI GCAATG_nn'
Cuts at: 0 588 1254
Size: 588 666
BsrFI r'CCGG_y
Cuts at: 0 308 1069 1254
Size: 308 761 185
Fragments arranged by size:
761 308 185
BsrGI T'GTAC_A
Cuts at: 0 939 1254
Size: 939 315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 943 1254
Size: 943 311
BstYI r'GATC_y
Cuts at: 0 1142 1254
Size: 1142 112
Bsu36I CC'TnA_GG
Cuts at: 0 140 188 1254
Size: 140 48 1066
Fragments arranged by size:
1066 140 48
BtgI C'CryG_G
Cuts at: 0 913 1254
Size: 913 341
DraI TTT'AAA
Cuts at: 0 132 1254
Size: 132 1122
EaeI y'GGCC_r
Cuts at: 0 306 933 1254
Size: 306 627 321
Fragments arranged by size:
627 321 306
EarI CTCTTCn'nnn_
Cuts at: 0 877 1254
Size: 877 377
EcoO109I rG'GnC_Cy
Cuts at: 0 1243 1254
Size: 1243 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 806 838 1254
Size: 367 32 407 32 416
Fragments arranged by size:
416 407 367 32 32
HincII GTy'rAC
Cuts at: 0 314 1254
Size: 314 940
HindIII A'AGCT_T
Cuts at: 0 806 1254
Size: 806 448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1102 1254
Size: 1102 152
MslI CAynn'nnrTG
Cuts at: 0 292 753 941 1254
Size: 292 461 188 313
Fragments arranged by size:
461 313 292 188
NaeI GCC'GGC
Cuts at: 0 1071 1254
Size: 1071 183
NcoI C'CATG_G
Cuts at: 0 913 1254
Size: 913 341
NdeI CA'TA_TG
Cuts at: 0 424 1254
Size: 424 830
NgoMIV G'CCGG_C
Cuts at: 0 1069 1254
Size: 1069 185
NsiI A_TGCA'T
Cuts at: 0 575 1254
Size: 575 679
NspI r_CATG'y
Cuts at: 0 1108 1254
Size: 1108 146
PflMI CCAn_nnn'nTGG
Cuts at: 0 258 583 1254
Size: 258 325 671
Fragments arranged by size:
671 325 258
PfoI T'CCnGG_A
Cuts at: 0 354 1254
Size: 354 900
PpuMI rG'GwC_Cy
Cuts at: 0 1243 1254
Size: 1243 11
PspOMI G'GGCC_C
Cuts at: 0 248 1254
Size: 248 1006
PstI C_TGCA'G
Cuts at: 0 418 468 768 1254
Size: 418 50 300 486
Fragments arranged by size:
486 418 300 50
SalI G'TCGA_C
Cuts at: 0 312 1254
Size: 312 942
SfcI C'TryA_G
Cuts at: 0 414 464 764 848 1254
Size: 414 50 300 84 406
Fragments arranged by size:
414 406 300 84 50
StuI AGG'CCT
Cuts at: 0 773 1254
Size: 773 481
StyI C'CwwG_G
Cuts at: 0 913 1254
Size: 913 341
TatI w'GTAC_w
Cuts at: 0 181 387 939 1254
Size: 181 206 552 315
Fragments arranged by size:
552 315 206 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 674 706 1046 1078 1254
Size: 674 32 340 32 176
Fragments arranged by size:
674 340 176 32 32
XbaI T'CTAG_A
Cuts at: 0 1248 1254
Size: 1248 6
Enzymes that do cut:
AccI ApaI ApaLI AvaI BanII BbsI BcgI BclI
BfrBI BlpI Bme1580I BmrI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BsrDI BsrFI
BsrGI BstXI BstYI Bsu36I BtgI DraI EaeI EarI
EcoO109I Hin4I HincII HindIII MmeI MslI NaeI NcoI
NdeI NgoMIV NsiI NspI PflMI PfoI PpuMI PspOMI
PstI SalI SfcI StuI StyI TatI TstI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BciVI BglI BglII BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MscI
MspA1I NarI NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII Tth111I XcmI
XhoI XmaI XmnI ZraI