Restriction map for
pFN21AE3179
(Linear) (Six-base) MAPSORT of: KIEE3179.seq Check: 5202 from: 1 to: 1248
KIEE3179.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AccI GT'mk_AC
Cuts at: 0 192 1248
Size: 192 1056
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1151 1248
Size: 908 243 97
Fragments arranged by size:
908 243 97
AflII C'TTAA_G
Cuts at: 0 660 1248
Size: 660 588
AflIII A'CryG_T
Cuts at: 0 167 1248
Size: 167 1081
ApaI G_GGCC'C
Cuts at: 0 46 250 1248
Size: 46 204 998
Fragments arranged by size:
998 204 46
ApoI r'AATT_y
Cuts at: 0 262 406 704 1248
Size: 262 144 298 544
Fragments arranged by size:
544 298 262 144
AseI AT'TA_AT
Cuts at: 0 508 1248
Size: 508 740
BanI G'GyrC_C
Cuts at: 0 36 91 1248
Size: 36 55 1157
Fragments arranged by size:
1157 55 36
BanII G_rGCy'C
Cuts at: 0 46 250 1248
Size: 46 204 998
Fragments arranged by size:
998 204 46
BbeI G_GCGC'C
Cuts at: 0 40 1248
Size: 40 1208
BbsI GAAGACnn'nnnn_
Cuts at: 0 914 1248
Size: 914 334
Bme1580I G_kGCm'C
Cuts at: 0 46 94 143 167 250 1248
Size: 46 48 49 24 83 998
Fragments arranged by size:
998 83 49 48 46 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1039 1248
Size: 618 421 209
Fragments arranged by size:
618 421 209
BsaHI Gr'CG_yC
Cuts at: 0 37 1248
Size: 37 1211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 1248
Size: 347 901
BsiEI CG_ry'CG
Cuts at: 0 74 1248
Size: 74 1174
Bsp1286I G_dGCh'C
Cuts at: 0 46 94 143 167 250 1248
Size: 46 48 49 24 83 998
Fragments arranged by size:
998 83 49 48 46 24
BsrFI r'CCGG_y
Cuts at: 0 66 102 1248
Size: 66 36 1146
Fragments arranged by size:
1146 66 36
BstBI TT'CG_AA
Cuts at: 0 1230 1248
Size: 1230 18
BstEII G'GTnAC_C
Cuts at: 0 350 1248
Size: 350 898
BstZ17I GTA'TAC
Cuts at: 0 193 1248
Size: 193 1055
Bsu36I CC'TnA_GG
Cuts at: 0 86 1248
Size: 86 1162
BtsI GCAGTG_nn'
Cuts at: 0 359 718 1248
Size: 359 359 530
Fragments arranged by size:
530 359 359
DraI TTT'AAA
Cuts at: 0 1061 1248
Size: 1061 187
EaeI y'GGCC_r
Cuts at: 0 158 1248
Size: 158 1090
EarI CTCTTCn'nnn_
Cuts at: 0 509 1248
Size: 509 739
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 908 1039 1151 1248
Size: 618 290 131 112 97
Fragments arranged by size:
618 290 131 112 97
EcoO109I rG'GnC_Cy
Cuts at: 0 43 246 1248
Size: 43 203 1002
Fragments arranged by size:
1002 203 43
EcoRI G'AATT_C
Cuts at: 0 262 1248
Size: 262 986
HaeII r_GCGC'y
Cuts at: 0 40 1248
Size: 40 1208
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 33 534 566 616 648 1248
Size: 1 32 501 32 50 32 600
Fragments arranged by size:
600 501 50 32 32 32 1
HindIII A'AGCT_T
Cuts at: 0 600 936 1248
Size: 600 336 312
Fragments arranged by size:
600 336 312
KasI G'GCGC_C
Cuts at: 0 36 1248
Size: 36 1212
MslI CAynn'nnrTG
Cuts at: 0 206 1061 1248
Size: 206 855 187
Fragments arranged by size:
855 206 187
NaeI GCC'GGC
Cuts at: 0 104 1248
Size: 104 1144
NarI GG'CG_CC
Cuts at: 0 37 1248
Size: 37 1211
NgoMIV G'CCGG_C
Cuts at: 0 102 1248
Size: 102 1146
NspI r_CATG'y
Cuts at: 0 171 1047 1248
Size: 171 876 201
Fragments arranged by size:
876 201 171
PciI A'CATG_T
Cuts at: 0 167 1248
Size: 167 1081
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 508 540 1248
Size: 508 32 708
Fragments arranged by size:
708 508 32
PshAI GACnn'nnGTC
Cuts at: 0 846 1248
Size: 846 402
PspOMI G'GGCC_C
Cuts at: 0 42 246 1248
Size: 42 204 1002
Fragments arranged by size:
1002 204 42
PstI C_TGCA'G
Cuts at: 0 713 1248
Size: 713 535
PvuI CG_AT'CG
Cuts at: 0 74 1248
Size: 74 1174
ScaI AGT'ACT
Cuts at: 0 863 1248
Size: 863 385
SfcI C'TryA_G
Cuts at: 0 709 1248
Size: 709 539
SfoI GGC'GCC
Cuts at: 0 38 1248
Size: 38 1210
SgrAI Cr'CCGG_yG
Cuts at: 0 66 1248
Size: 66 1182
SmlI C'TyrA_G
Cuts at: 0 660 1248
Size: 660 588
SspI AAT'ATT
Cuts at: 0 325 337 1248
Size: 325 12 911
Fragments arranged by size:
911 325 12
StyI C'CwwG_G
Cuts at: 0 241 1248
Size: 241 1007
SwaI ATTT'AAAT
Cuts at: 0 1061 1248
Size: 1061 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1022 1248
Size: 1022 226
TatI w'GTAC_w
Cuts at: 0 861 869 1071 1224 1248
Size: 861 8 202 153 24
Fragments arranged by size:
861 202 153 24 8
XbaI T'CTAG_A
Cuts at: 0 309 1248
Size: 309 939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1108 1248
Size: 1108 140
Enzymes that do cut:
AccI AcuI AflII AflIII ApaI ApoI AseI BanI
BanII BbeI BbsI Bme1580I BpmI BsaHI BsgI BsiEI
Bsp1286I BsrFI BstBI BstEII BstZ17I Bsu36I BtsI DraI
EaeI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII
KasI MslI NaeI NarI NgoMIV NspI PciI PpiI
PshAI PspOMI PstI PvuI ScaI SfcI SfoI SgrAI
SmlI SspI StyI SwaI TaqII TatI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MmeI MscI MspA1I NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpuMI PsiI PspXI PsrI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SgfI
SmaI SnaBI SpeI SphI SrfI StuI TstI Tth111I
XhoI XmaI XmnI ZraI