Restriction map for
pFN21AE3178
(Linear) (Six-base) MAPSORT of: KIEE3178.seq Check: 5731 from: 1 to: 1245
KIEE3178.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 209 1172 1245
Size: 43 166 963 73
Fragments arranged by size:
963 166 73 43
AflIII A'CryG_T
Cuts at: 0 908 1245
Size: 908 337
ApoI r'AATT_y
Cuts at: 0 664 1245
Size: 664 581
AseI AT'TA_AT
Cuts at: 0 494 1245
Size: 494 751
AvrII C'CTAG_G
Cuts at: 0 861 1245
Size: 861 384
BamHI G'GATC_C
Cuts at: 0 300 1245
Size: 300 945
BanI G'GyrC_C
Cuts at: 0 33 1130 1245
Size: 33 1097 115
Fragments arranged by size:
1097 115 33
BanII G_rGCy'C
Cuts at: 0 295 1245
Size: 295 950
BbeI G_GCGC'C
Cuts at: 0 1134 1245
Size: 1134 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 788 842 1245
Size: 788 54 403
Fragments arranged by size:
788 403 54
BfrBI ATG'CAT
Cuts at: 0 118 598 1245
Size: 118 480 647
Fragments arranged by size:
647 480 118
BglI GCCn_nnn'nGGC
Cuts at: 0 961 1245
Size: 961 284
BglII A'GATC_T
Cuts at: 0 1020 1245
Size: 1020 225
Bme1580I G_kGCm'C
Cuts at: 0 38 713 1245
Size: 38 675 532
Fragments arranged by size:
675 532 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 958 1245
Size: 629 329 287
Fragments arranged by size:
629 329 287
BsaHI Gr'CG_yC
Cuts at: 0 1131 1245
Size: 1131 114
BsaWI w'CCGG_w
Cuts at: 0 11 1245
Size: 11 1234
BseYI C'CCAG_C
Cuts at: 0 931 1174 1245
Size: 931 243 71
Fragments arranged by size:
931 243 71
Bsp1286I G_dGCh'C
Cuts at: 0 38 295 713 1245
Size: 38 257 418 532
Fragments arranged by size:
532 418 257 38
BsrBI CCG'CTC
Cuts at: 0 78 1245
Size: 78 1167
BsrFI r'CCGG_y
Cuts at: 0 30 1245
Size: 30 1215
BstAPI GCAn_nnn'nTGC
Cuts at: 0 33 1245
Size: 33 1212
BstBI TT'CG_AA
Cuts at: 0 882 1245
Size: 882 363
BstYI r'GATC_y
Cuts at: 0 300 1020 1245
Size: 300 720 225
Fragments arranged by size:
720 300 225
Bsu36I CC'TnA_GG
Cuts at: 0 296 1245
Size: 296 949
BtgI C'CryG_G
Cuts at: 0 1037 1245
Size: 1037 208
BtsI GCAGTG_nn'
Cuts at: 0 1185 1245
Size: 1185 60
ClaI AT'CG_AT
Cuts at: 0 560 1245
Size: 560 685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 293 328 1245
Size: 293 35 917
Fragments arranged by size:
917 293 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 595 1245
Size: 595 650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 209 629 958 1172 1245
Size: 43 166 420 329 214 73
Fragments arranged by size:
420 329 214 166 73 43
EcoRI G'AATT_C
Cuts at: 0 664 1245
Size: 664 581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 211 1245
Size: 211 1034
HaeII r_GCGC'y
Cuts at: 0 682 1134 1245
Size: 682 452 111
Fragments arranged by size:
682 452 111
KasI G'GCGC_C
Cuts at: 0 1130 1245
Size: 1130 115
MslI CAynn'nnrTG
Cuts at: 0 359 1245
Size: 359 886
NarI GG'CG_CC
Cuts at: 0 1131 1245
Size: 1131 114
NcoI C'CATG_G
Cuts at: 0 1037 1245
Size: 1037 208
NdeI CA'TA_TG
Cuts at: 0 600 728 1245
Size: 600 128 517
Fragments arranged by size:
600 517 128
NsiI A_TGCA'T
Cuts at: 0 120 600 1245
Size: 120 480 645
Fragments arranged by size:
645 480 120
NspI r_CATG'y
Cuts at: 0 122 489 912 1245
Size: 122 367 423 333
Fragments arranged by size:
423 367 333 122
PasI CC'CwG_GG
Cuts at: 0 1187 1245
Size: 1187 58
PciI A'CATG_T
Cuts at: 0 908 1245
Size: 908 337
PfoI T'CCnGG_A
Cuts at: 0 95 1245
Size: 95 1150
SexAI A'CCwGG_T
Cuts at: 0 635 1245
Size: 635 610
SfoI GGC'GCC
Cuts at: 0 1132 1245
Size: 1132 113
SphI G_CATG'C
Cuts at: 0 489 1245
Size: 489 756
SspI AAT'ATT
Cuts at: 0 445 1245
Size: 445 800
StyI C'CwwG_G
Cuts at: 0 861 1037 1245
Size: 861 176 208
Fragments arranged by size:
861 208 176
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 573 1044 1245
Size: 573 471 201
Fragments arranged by size:
573 471 201
Enzymes that do cut:
AcuI AflIII ApoI AseI AvrII BamHI BanI BanII
BbeI BbsI BfrBI BglI BglII Bme1580I BpmI BsaHI
BsaWI BseYI Bsp1286I BsrBI BsrFI BstAPI BstBI BstYI
Bsu36I BtgI BtsI ClaI CspCI EciI Eco57MI EcoRI
FalI HaeII KasI MslI NarI NcoI NdeI NsiI
NspI PasI PciI PfoI SexAI SfoI SphI SspI
StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI BaeI BbvCI BcgI BciVI BclI BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaXI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI
BstEII BstXI BstZ17I BtgZI DraI DraIII DrdI EaeI
EagI EarI EcoICRI EcoNI EcoO109I EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NgoMIV NheI NotI NruI
PacI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI