Restriction map for
pFN21AE3176
(Linear) (Six-base) MAPSORT of: KIEE3176.seq Check: 3925 from: 1 to: 1230
KIEE3176.orf
With 182 enzymes: SgfI *
March 22, 2012 16:18 ..
Acc65I G'GTAC_C
Cuts at: 0 199 1230
Size: 199 1031
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1230
Size: 667 563
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 130 162 933 965 1230
Size: 130 32 771 32 265
Fragments arranged by size:
771 265 130 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 359 1168 1230
Size: 359 809 62
Fragments arranged by size:
809 359 62
ApaI G_GGCC'C
Cuts at: 0 924 1230
Size: 924 306
ApaLI G'TGCA_C
Cuts at: 0 211 1230
Size: 211 1019
AvaI C'yCGr_G
Cuts at: 0 134 1230
Size: 134 1096
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 183 216 1230
Size: 183 33 1014
Fragments arranged by size:
1014 183 33
BamHI G'GATC_C
Cuts at: 0 1202 1230
Size: 1202 28
BanI G'GyrC_C
Cuts at: 0 199 1230
Size: 199 1031
BanII G_rGCy'C
Cuts at: 0 59 281 415 443 893 902 924 1017
Size: 59 222 134 28 450 9 22 93
Cuts at: 1017 1230
Size: 213
Fragments arranged by size:
450 222 213 134 93 59 28 22
9
BbsI GAAGACnn'nnnn_
Cuts at: 0 482 1230
Size: 482 748
BbvCI CC'TCA_GC
Cuts at: 0 729 1230
Size: 729 501
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 108 142 1230
Size: 108 34 1088
Fragments arranged by size:
1088 108 34
BfrBI ATG'CAT
Cuts at: 0 679 1230
Size: 679 551
BglI GCCn_nnn'nGGC
Cuts at: 0 391 1230
Size: 391 839
Bme1580I G_kGCm'C
Cuts at: 0 83 215 924 1230
Size: 83 132 709 306
Fragments arranged by size:
709 306 132 83
BmrI ACTGGGnnnn_n'
Cuts at: 0 253 366 1230
Size: 253 113 864
Fragments arranged by size:
864 253 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 313 440 916 1230
Size: 73 240 127 476 314
Fragments arranged by size:
476 314 240 127 73
Bpu10I CC'TnA_GC
Cuts at: 0 729 1230
Size: 729 501
BsaI GGTCTCn'nnnn_
Cuts at: 0 294 1081 1230
Size: 294 787 149
Fragments arranged by size:
787 294 149
BsaWI w'CCGG_w
Cuts at: 0 881 1230
Size: 881 349
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 130 160 933 963 1230
Size: 130 30 773 30 267
Fragments arranged by size:
773 267 130 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 700 916 1230
Size: 700 216 314
Fragments arranged by size:
700 314 216
BseYI C'CCAG_C
Cuts at: 0 386 1230
Size: 386 844
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 67 345 892 1230
Size: 49 18 278 547 338
Fragments arranged by size:
547 338 278 49 18
BsiHKAI G_wGCw'C
Cuts at: 0 215 562 727 893 1145 1230
Size: 215 347 165 166 252 85
Fragments arranged by size:
347 252 215 166 165 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 135 1230
Size: 135 1095
Bsp1286I G_dGCh'C
Cuts at: 0 59 83 215 281 415 443 562 727
Size: 59 24 132 66 134 28 119 165
Cuts at: 727 893 902 924 1017 1145 1230
Size: 166 9 22 93 128 85
Fragments arranged by size:
166 165 134 132 128 119 93 85
66 59 28 24 22 9
BspEI T'CCGG_A
Cuts at: 0 881 1230
Size: 881 349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 753 1230
Size: 753 477
BsrDI GCAATG_nn'
Cuts at: 0 354 535 1230
Size: 354 181 695
Fragments arranged by size:
695 354 181
BsrFI r'CCGG_y
Cuts at: 0 340 1230
Size: 340 890
BssSI C'ACGA_G
Cuts at: 0 1036 1230
Size: 1036 194
BstAPI GCAn_nnn'nTGC
Cuts at: 0 359 1230
Size: 359 871
BstYI r'GATC_y
Cuts at: 0 642 1202 1230
Size: 642 560 28
Fragments arranged by size:
642 560 28
BtgI C'CryG_G
Cuts at: 0 517 1056 1230
Size: 517 539 174
Fragments arranged by size:
539 517 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 686 1230
Size: 686 544
EaeI y'GGCC_r
Cuts at: 0 167 311 376 416 1230
Size: 167 144 65 40 814
Fragments arranged by size:
814 167 144 65 40
EarI CTCTTCn'nnn_
Cuts at: 0 431 499 1230
Size: 431 68 731
Fragments arranged by size:
731 431 68
EcoICRI GAG'CTC
Cuts at: 0 891 1230
Size: 891 339
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 313 440 667 916 1230
Size: 73 240 127 227 249 314
Fragments arranged by size:
314 249 240 227 127 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 1230
Size: 67 1163
EcoO109I rG'GnC_Cy
Cuts at: 0 522 656 921 1230
Size: 522 134 265 309
Fragments arranged by size:
522 309 265 134
EcoRV GAT'ATC
Cuts at: 0 1215 1230
Size: 1215 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 804 1230
Size: 804 426
FspI TGC'GCA
Cuts at: 0 719 748 1230
Size: 719 29 482
Fragments arranged by size:
719 482 29
FspAI rTGC'GCAy
Cuts at: 0 748 1230
Size: 748 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 833 865 1230
Size: 664 32 137 32 365
Fragments arranged by size:
664 365 137 32 32
HincII GTy'rAC
Cuts at: 0 1089 1230
Size: 1089 141
KpnI G_GTAC'C
Cuts at: 0 203 1230
Size: 203 1027
MscI TGG'CCA
Cuts at: 0 169 313 1230
Size: 169 144 917
Fragments arranged by size:
917 169 144
MslI CAynn'nnrTG
Cuts at: 0 88 632 671 1096 1230
Size: 88 544 39 425 134
Fragments arranged by size:
544 425 134 88 39
MspA1I CmG'CkG
Cuts at: 0 51 390 1230
Size: 51 339 840
Fragments arranged by size:
840 339 51
NdeI CA'TA_TG
Cuts at: 0 316 1230
Size: 316 914
NsiI A_TGCA'T
Cuts at: 0 681 1230
Size: 681 549
NspI r_CATG'y
Cuts at: 0 385 762 1230
Size: 385 377 468
Fragments arranged by size:
468 385 377
PflMI CCAn_nnn'nTGG
Cuts at: 0 1168 1230
Size: 1168 62
PfoI T'CCnGG_A
Cuts at: 0 638 893 1230
Size: 638 255 337
Fragments arranged by size:
638 337 255
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 933 965 1230
Size: 130 32 771 32 265
Fragments arranged by size:
771 265 130 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 656 1230
Size: 656 574
PspOMI G'GGCC_C
Cuts at: 0 920 1230
Size: 920 310
PstI C_TGCA'G
Cuts at: 0 1211 1230
Size: 1211 19
PvuII CAG'CTG
Cuts at: 0 51 390 1230
Size: 51 339 840
Fragments arranged by size:
840 339 51
SacI G_AGCT'C
Cuts at: 0 893 1230
Size: 893 337
SanDI GG'GwC_CC
Cuts at: 0 656 1230
Size: 656 574
SapI GCTCTTCn'nnn_
Cuts at: 0 431 499 1230
Size: 431 68 731
Fragments arranged by size:
731 431 68
SbfI CC_TGCA'GG
Cuts at: 0 1211 1230
Size: 1211 19
ScaI AGT'ACT
Cuts at: 0 787 808 1230
Size: 787 21 422
Fragments arranged by size:
787 422 21
SexAI A'CCwGG_T
Cuts at: 0 68 491 1230
Size: 68 423 739
Fragments arranged by size:
739 423 68
SfcI C'TryA_G
Cuts at: 0 1207 1230
Size: 1207 23
SmaI CCC'GGG
Cuts at: 0 136 1230
Size: 136 1094
SphI G_CATG'C
Cuts at: 0 385 762 1230
Size: 385 377 468
Fragments arranged by size:
468 385 377
StyI C'CwwG_G
Cuts at: 0 404 419 1082 1230
Size: 404 15 663 148
Fragments arranged by size:
663 404 148 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 417 730 1230
Size: 417 313 500
Fragments arranged by size:
500 417 313
TatI w'GTAC_w
Cuts at: 0 191 620 785 806 1230
Size: 191 429 165 21 424
Fragments arranged by size:
429 424 191 165 21
XmaI C'CCGG_G
Cuts at: 0 134 1230
Size: 134 1096
XmnI GAAnn'nnTTC
Cuts at: 0 178 508 1230
Size: 178 330 722
Fragments arranged by size:
722 330 178
Enzymes that do cut:
Acc65I AcuI AloI AlwNI ApaI ApaLI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BcgI BfrBI BglI
Bme1580I BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrFI BssSI BstAPI BstYI BtgI BtgZI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI FspAI
Hin4I HincII KpnI MscI MslI MspA1I NdeI NsiI
NspI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SbfI ScaI SexAI SfcI SmaI
SphI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApoI AscI AseI AsiSI AvrII
BbeI BciVI BclI BglII BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BspHI
BsrBI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI FseI HaeII HindIII HpaI KasI MfeI MluI
MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI ZraI