(Linear) (Six-base) MAPSORT of: KIEE3172.seq  Check: 194  from: 1  to: 1209



KIEE3172.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:34  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     194     359    1209 
   Size:         24     170     165     850

  Fragments arranged by size: 

                 850     170     165      24
 
AflIII A'CryG_T

Cuts at:      0     116    1209 
   Size:        116    1093
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1017    1209 
   Size:       1017     192
 
BanI G'GyrC_C

Cuts at:      0      63     583    1209 
   Size:         63     520     626

  Fragments arranged by size: 

                 626     520      63
 
BanII G_rGCy'C

Cuts at:      0     350    1209 
   Size:        350     859
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     113    1209 
   Size:        113    1096
 
Bme1580I G_kGCm'C

Cuts at:      0     588    1209 
   Size:        588     621
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1174    1209 
   Size:       1174      35
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231    1157    1209 
   Size:        231     926      52

  Fragments arranged by size: 

                 926     231      52
 
BsaAI yAC'GTr

Cuts at:      0    1072    1209 
   Size:       1072     137
 
BsaBI GATnn'nnATC

Cuts at:      0     263    1209 
   Size:        263     946
 
BseYI C'CCAG_C

Cuts at:      0     674    1209 
   Size:        674     535
 
BsiHKAI G_wGCw'C

Cuts at:      0     350    1209 
   Size:        350     859
 
BsmI GAATG_Cn'

Cuts at:      0    1006    1167    1209 
   Size:       1006     161      42

  Fragments arranged by size: 

                1006     161      42
 
Bsp1286I G_dGCh'C

Cuts at:      0     350     588    1209 
   Size:        350     238     621

  Fragments arranged by size: 

                 621     350     238
 
BsrDI GCAATG_nn'

Cuts at:      0     575    1209 
   Size:        575     634
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      60    1209 
   Size:         60    1149
 
BstYI r'GATC_y

Cuts at:      0     264    1209 
   Size:        264     945
 
DraI TTT'AAA

Cuts at:      0     398    1209 
   Size:        398     811
 
EarI CTCTTCn'nnn_

Cuts at:      0      79     170     338    1209 
   Size:         79      91     168     871

  Fragments arranged by size: 

                 871     168      91      79
 
EcoICRI GAG'CTC

Cuts at:      0     348    1209 
   Size:        348     861
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24     194     359    1174    1209 
   Size:         24     170     165     815      35

  Fragments arranged by size: 

                 815     170     165      35      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     970    1209 
   Size:        970     239
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     167    1209 
   Size:        167    1042
 
MslI CAynn'nnrTG

Cuts at:      0     295     568    1209 
   Size:        295     273     641

  Fragments arranged by size: 

                 641     295     273
 
MspA1I CmG'CkG

Cuts at:      0     136    1209 
   Size:        136    1073
 
NspI r_CATG'y

Cuts at:      0     120    1209 
   Size:        120    1089
 
PciI A'CATG_T

Cuts at:      0     116    1209 
   Size:        116    1093
 
PstI C_TGCA'G

Cuts at:      0     876    1209 
   Size:        876     333
 
PvuII CAG'CTG

Cuts at:      0     136    1209 
   Size:        136    1073
 
SacI G_AGCT'C

Cuts at:      0     350    1209 
   Size:        350     859
 
SapI GCTCTTCn'nnn_

Cuts at:      0     170     338    1209 
   Size:        170     168     871

  Fragments arranged by size: 

                 871     170     168
 
SexAI A'CCwGG_T

Cuts at:      0      52    1209 
   Size:         52    1157
 
SfcI C'TryA_G

Cuts at:      0     872    1209 
   Size:        872     337
 
SmlI C'TyrA_G

Cuts at:      0     246    1172    1209 
   Size:        246     926      37

  Fragments arranged by size: 

                 926     246      37
 
SnaBI TAC'GTA

Cuts at:      0    1072    1209 
   Size:       1072     137
 
SspI AAT'ATT

Cuts at:      0     394     403    1021    1209 
   Size:        394       9     618     188

  Fragments arranged by size: 

                 618     394     188       9
 
StyI C'CwwG_G

Cuts at:      0     971    1209 
   Size:        971     238
 
TatI w'GTAC_w

Cuts at:      0     160    1209 
   Size:        160    1049
 
XmnI GAAnn'nnTTC

Cuts at:      0    1004    1209 
   Size:       1004     205
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI     BanI    BanII     BbsI Bme1580I     BpmI
    BpuEI    BsaAI    BsaBI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI
    BstXI    BstYI     DraI     EarI  EcoICRI  Eco57MI    EcoNI     MmeI
     MslI   MspA1I     NspI     PciI     PstI    PvuII     SacI     SapI
    SexAI     SfcI     SmlI    SnaBI     SspI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI