Restriction map for
pFN21AE3167
(Linear) (Six-base) MAPSORT of: KIEE3167.seq Check: 277 from: 1 to: 1176
KIEE3167.orf
With 182 enzymes: SgfI *
March 22, 2012 16:18 ..
Acc65I G'GTAC_C
Cuts at: 0 328 1176
Size: 328 848
AleI CACnn'nnGTG
Cuts at: 0 280 1176
Size: 280 896
AlwNI CAG_nnn'CTG
Cuts at: 0 348 1176
Size: 348 828
ApaI G_GGCC'C
Cuts at: 0 69 1176
Size: 69 1107
ApoI r'AATT_y
Cuts at: 0 23 739 1176
Size: 23 716 437
Fragments arranged by size:
716 437 23
AvrII C'CTAG_G
Cuts at: 0 186 1176
Size: 186 990
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 312 345 1176
Size: 312 33 831
Fragments arranged by size:
831 312 33
BamHI G'GATC_C
Cuts at: 0 602 1176
Size: 602 574
BanI G'GyrC_C
Cuts at: 0 245 328 1176
Size: 245 83 848
Fragments arranged by size:
848 245 83
BanII G_rGCy'C
Cuts at: 0 69 461 659 1176
Size: 69 392 198 517
Fragments arranged by size:
517 392 198 69
BbvCI CC'TCA_GC
Cuts at: 0 110 821 1176
Size: 110 711 355
Fragments arranged by size:
711 355 110
BglI GCCn_nnn'nGGC
Cuts at: 0 73 106 1176
Size: 73 33 1070
Fragments arranged by size:
1070 73 33
Bme1580I G_kGCm'C
Cuts at: 0 69 248 1176
Size: 69 179 928
Fragments arranged by size:
928 179 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1176
Size: 232 944
Bpu10I CC'TnA_GC
Cuts at: 0 110 821 1176
Size: 110 711 355
Fragments arranged by size:
711 355 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1176
Size: 673 503
BsaI GGTCTCn'nnnn_
Cuts at: 0 997 1176
Size: 997 179
BsaBI GATnn'nnATC
Cuts at: 0 553 1176
Size: 553 623
BsaWI w'CCGG_w
Cuts at: 0 788 1176
Size: 788 388
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 775 1176
Size: 151 624 401
Fragments arranged by size:
624 401 151
BseYI C'CCAG_C
Cuts at: 0 906 1176
Size: 906 270
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 1176
Size: 487 689
BsiHKAI G_wGCw'C
Cuts at: 0 659 1176
Size: 659 517
BsmI GAATG_Cn'
Cuts at: 0 192 725 1176
Size: 192 533 451
Fragments arranged by size:
533 451 192
Bsp1286I G_dGCh'C
Cuts at: 0 69 248 461 659 1176
Size: 69 179 213 198 517
Fragments arranged by size:
517 213 198 179 69
BspEI T'CCGG_A
Cuts at: 0 788 1176
Size: 788 388
BsrDI GCAATG_nn'
Cuts at: 0 683 926 1176
Size: 683 243 250
Fragments arranged by size:
683 250 243
BsrFI r'CCGG_y
Cuts at: 0 221 1176
Size: 221 955
BsrGI T'GTAC_A
Cuts at: 0 1130 1165 1176
Size: 1130 35 11
Fragments arranged by size:
1130 35 11
BssSI C'ACGA_G
Cuts at: 0 43 1176
Size: 43 1133
BstAPI GCAn_nnn'nTGC
Cuts at: 0 198 1176
Size: 198 978
BstXI CCAn_nnnn'nTGG
Cuts at: 0 756 1176
Size: 756 420
BstYI r'GATC_y
Cuts at: 0 344 602 1176
Size: 344 258 574
Fragments arranged by size:
574 344 258
BtgI C'CryG_G
Cuts at: 0 278 1176
Size: 278 898
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1001 1176
Size: 1001 175
BtsI GCAGTG_nn'
Cuts at: 0 196 1176
Size: 196 980
DrdI GACnn_nn'nnGTC
Cuts at: 0 1051 1176
Size: 1051 125
EarI CTCTTCn'nnn_
Cuts at: 0 788 840 1176
Size: 788 52 336
Fragments arranged by size:
788 336 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 889 1176
Size: 889 287
EcoICRI GAG'CTC
Cuts at: 0 657 1176
Size: 657 519
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1176
Size: 232 944
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 323 1176
Size: 133 190 853
Fragments arranged by size:
853 190 133
EcoO109I rG'GnC_Cy
Cuts at: 0 65 114 1176
Size: 65 49 1062
Fragments arranged by size:
1062 65 49
EcoRI G'AATT_C
Cuts at: 0 23 1176
Size: 23 1153
EcoRV GAT'ATC
Cuts at: 0 555 1176
Size: 555 621
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 587 619 799 831 915 947 1176
Size: 587 32 180 32 84 32 229
Fragments arranged by size:
587 229 180 84 32 32 32
KpnI G_GTAC'C
Cuts at: 0 332 1176
Size: 332 844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 1176
Size: 447 729
MslI CAynn'nnrTG
Cuts at: 0 280 1176
Size: 280 896
MspA1I CmG'CkG
Cuts at: 0 232 820 906 1176
Size: 232 588 86 270
Fragments arranged by size:
588 270 232 86
NcoI C'CATG_G
Cuts at: 0 278 1176
Size: 278 898
NspI r_CATG'y
Cuts at: 0 210 1172 1176
Size: 210 962 4
Fragments arranged by size:
962 210 4
PasI CC'CwG_GG
Cuts at: 0 461 749 1176
Size: 461 288 427
Fragments arranged by size:
461 427 288
PspOMI G'GGCC_C
Cuts at: 0 65 1176
Size: 65 1111
PvuII CAG'CTG
Cuts at: 0 232 820 906 1176
Size: 232 588 86 270
Fragments arranged by size:
588 270 232 86
SacI G_AGCT'C
Cuts at: 0 659 1176
Size: 659 517
ScaI AGT'ACT
Cuts at: 0 980 1176
Size: 980 196
SmlI C'TyrA_G
Cuts at: 0 652 1176
Size: 652 524
SpeI A'CTAG_T
Cuts at: 0 387 1176
Size: 387 789
StuI AGG'CCT
Cuts at: 0 318 1176
Size: 318 858
StyI C'CwwG_G
Cuts at: 0 169 186 278 677 1176
Size: 169 17 92 399 499
Fragments arranged by size:
499 399 169 92 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 453 1176
Size: 453 723
TatI w'GTAC_w
Cuts at: 0 978 1130 1165 1176
Size: 978 152 35 11
Fragments arranged by size:
978 152 35 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 478 510 1176
Size: 478 32 666
Fragments arranged by size:
666 478 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 1176
Size: 285 891
XmnI GAAnn'nnTTC
Cuts at: 0 782 1176
Size: 782 394
Enzymes that do cut:
Acc65I AleI AlwNI ApaI ApoI AvrII BaeI BamHI
BanI BanII BbvCI BglI Bme1580I BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BsrDI BsrFI BsrGI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI DrdI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I KpnI MmeI
MslI MspA1I NcoI NspI PasI PspOMI PvuII SacI
ScaI SmlI SpeI StuI StyI TaqII TatI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI
AvaI BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BsaAI BsaHI BsaXI
BsiEI BsiWI BsmBI BspHI BspMI BsrBI BssHII BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EaeI
EagI FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI SwaI Tth111I XbaI XhoI
XmaI ZraI