Restriction map for
pFN21AE3162
(Linear) (Six-base) MAPSORT of: KIEE3162.seq Check: 9850 from: 1 to: 1140
KIEE3162.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 541 1140
Size: 146 395 599
Fragments arranged by size:
599 395 146
AfeI AGC'GCT
Cuts at: 0 526 1140
Size: 526 614
AflIII A'CryG_T
Cuts at: 0 824 953 1106 1140
Size: 824 129 153 34
Fragments arranged by size:
824 153 129 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 117 1140
Size: 117 1023
AlwNI CAG_nnn'CTG
Cuts at: 0 711 1140
Size: 711 429
ApaI G_GGCC'C
Cuts at: 0 31 69 1140
Size: 31 38 1071
Fragments arranged by size:
1071 38 31
AvaI C'yCGr_G
Cuts at: 0 61 632 1140
Size: 61 571 508
Fragments arranged by size:
571 508 61
BanI G'GyrC_C
Cuts at: 0 456 628 685 927 1140
Size: 456 172 57 242 213
Fragments arranged by size:
456 242 213 172 57
BanII G_rGCy'C
Cuts at: 0 31 69 657 699 1140
Size: 31 38 588 42 441
Fragments arranged by size:
588 441 42 38 31
BbeI G_GCGC'C
Cuts at: 0 460 632 689 1140
Size: 460 172 57 451
Fragments arranged by size:
460 451 172 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 348 1140
Size: 348 792
BglI GCCn_nnn'nGGC
Cuts at: 0 434 683 1140
Size: 434 249 457
Fragments arranged by size:
457 434 249
BglII A'GATC_T
Cuts at: 0 764 1140
Size: 764 376
Bme1580I G_kGCm'C
Cuts at: 0 31 69 916 932 1140
Size: 31 38 847 16 208
Fragments arranged by size:
847 208 38 31 16
BmgBI CAC'GTC
Cuts at: 0 15 204 827 1140
Size: 15 189 623 313
Fragments arranged by size:
623 313 189 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 802 1140
Size: 802 338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1140
Size: 331 809
BsaAI yAC'GTr
Cuts at: 0 387 489 939 954 989 1128 1140
Size: 387 102 450 15 35 139 12
Fragments arranged by size:
450 387 139 102 35 15 12
BsaBI GATnn'nnATC
Cuts at: 0 946 1140
Size: 946 194
BsaHI Gr'CG_yC
Cuts at: 0 57 457 629 686 1140
Size: 57 400 172 57 454
Fragments arranged by size:
454 400 172 57 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 100 130 304 334 1140
Size: 100 30 174 30 806
Fragments arranged by size:
806 174 100 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 255 358 1140
Size: 255 103 782
Fragments arranged by size:
782 255 103
BseYI C'CCAG_C
Cuts at: 0 700 1140
Size: 700 440
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 1140
Size: 271 869
BsiEI CG_ry'CG
Cuts at: 0 650 1140
Size: 650 490
BsmI GAATG_Cn'
Cuts at: 0 749 1140
Size: 749 391
Bsp1286I G_dGCh'C
Cuts at: 0 31 69 657 699 916 932 1140
Size: 31 38 588 42 217 16 208
Fragments arranged by size:
588 217 208 42 38 31 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 254 332 1140
Size: 254 78 808
Fragments arranged by size:
808 254 78
BsrBI CCG'CTC
Cuts at: 0 1026 1140
Size: 1026 114
BsrFI r'CCGG_y
Cuts at: 0 97 132 1140
Size: 97 35 1008
Fragments arranged by size:
1008 97 35
BssHII G'CGCG_C
Cuts at: 0 608 1140
Size: 608 532
BstAPI GCAn_nnn'nTGC
Cuts at: 0 489 1140
Size: 489 651
BstYI r'GATC_y
Cuts at: 0 764 1140
Size: 764 376
BtgI C'CryG_G
Cuts at: 0 672 903 1140
Size: 672 231 237
Fragments arranged by size:
672 237 231
BtsI GCAGTG_nn'
Cuts at: 0 119 504 1140
Size: 119 385 636
Fragments arranged by size:
636 385 119
EaeI y'GGCC_r
Cuts at: 0 647 819 1140
Size: 647 172 321
Fragments arranged by size:
647 321 172
EagI C'GGCC_G
Cuts at: 0 647 1140
Size: 647 493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 331 541 1140
Size: 146 185 210 599
Fragments arranged by size:
599 210 185 146
EcoNI CCTnn'n_nnAGG
Cuts at: 0 313 1140
Size: 313 827
EcoO109I rG'GnC_Cy
Cuts at: 0 27 28 65 66 636 1092 1140
Size: 27 1 37 1 570 456 48
Fragments arranged by size:
570 456 48 37 27 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 1140
Size: 900 240
HaeII r_GCGC'y
Cuts at: 0 133 146 228 339 418 460 469 481
Size: 133 13 82 111 79 42 9 12
Cuts at: 481 499 528 632 689 1140
Size: 18 29 104 57 451
Fragments arranged by size:
451 133 111 104 82 79 57 42
29 18 13 12 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 1140
Size: 100 32 1008
Fragments arranged by size:
1008 100 32
KasI G'GCGC_C
Cuts at: 0 456 628 685 1140
Size: 456 172 57 455
Fragments arranged by size:
456 455 172 57
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1127 1140
Size: 1127 13
MslI CAynn'nnrTG
Cuts at: 0 937 952 1051 1140
Size: 937 15 99 89
Fragments arranged by size:
937 99 89 15
MspA1I CmG'CkG
Cuts at: 0 114 278 333 600 818 857 905 1140
Size: 114 164 55 267 218 39 48 235
Fragments arranged by size:
267 235 218 164 114 55 48 39
NaeI GCC'GGC
Cuts at: 0 99 134 1140
Size: 99 35 1006
Fragments arranged by size:
1006 99 35
NarI GG'CG_CC
Cuts at: 0 457 629 686 1140
Size: 457 172 57 454
Fragments arranged by size:
457 454 172 57
NgoMIV G'CCGG_C
Cuts at: 0 97 132 1140
Size: 97 35 1008
Fragments arranged by size:
1008 97 35
PasI CC'CwG_GG
Cuts at: 0 680 1140
Size: 680 460
PmlI CAC'GTG
Cuts at: 0 387 989 1140
Size: 387 602 151
Fragments arranged by size:
602 387 151
PpuMI rG'GwC_Cy
Cuts at: 0 636 1092 1140
Size: 636 456 48
Fragments arranged by size:
636 456 48
PspOMI G'GGCC_C
Cuts at: 0 27 65 1140
Size: 27 38 1075
Fragments arranged by size:
1075 38 27
PstI C_TGCA'G
Cuts at: 0 329 398 485 862 1140
Size: 329 69 87 377 278
Fragments arranged by size:
377 329 278 87 69
PvuII CAG'CTG
Cuts at: 0 857 1140
Size: 857 283
SacII CC_GC'GG
Cuts at: 0 906 1140
Size: 906 234
SanDI GG'GwC_CC
Cuts at: 0 636 1140
Size: 636 504
SfcI C'TryA_G
Cuts at: 0 325 394 481 858 897 1140
Size: 325 69 87 377 39 243
Fragments arranged by size:
377 325 243 87 69 39
SfoI GGC'GCC
Cuts at: 0 458 630 687 1140
Size: 458 172 57 453
Fragments arranged by size:
458 453 172 57
SmaI CCC'GGG
Cuts at: 0 63 634 1140
Size: 63 571 506
Fragments arranged by size:
571 506 63
SnaBI TAC'GTA
Cuts at: 0 1128 1140
Size: 1128 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 1140
Size: 313 827
XmaI C'CCGG_G
Cuts at: 0 61 632 1140
Size: 61 571 508
Fragments arranged by size:
571 508 61
XmnI GAAnn'nnTTC
Cuts at: 0 526 1140
Size: 526 614
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BglI BglII Bme1580I BmgBI BmrI
BpmI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BstYI BtgI BtsI EaeI EagI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI MmeI MslI MspA1I NaeI
NarI NgoMIV PasI PmlI PpuMI PspOMI PstI PvuII
SacII SanDI SfcI SfoI SmaI SnaBI TaqII XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI
BmtI BplI Bpu10I BpuEI BsaI BsaWI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EarI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SpeI SphI SrfI
SspI StuI StyI SwaI TatI TstI Tth111I XbaI
XcmI XhoI ZraI