(Linear) (Six-base) MAPSORT of: KIEE3161.seq  Check: 6975  from: 1  to: 1125



KIEE3161.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:33  ..

AccI GT'mk_AC

Cuts at:      0     719    1044    1125 
   Size:        719     325      81

  Fragments arranged by size: 

                 719     325      81
 
Acc65I G'GTAC_C

Cuts at:      0     949    1125 
   Size:        949     176
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     163     381    1125 
   Size:        149      14     218     744

  Fragments arranged by size: 

                 744     218     149      14
 
AgeI A'CCGG_T

Cuts at:      0      56    1125 
   Size:         56    1069
 
ApoI r'AATT_y

Cuts at:      0     846     923    1125 
   Size:        846      77     202

  Fragments arranged by size: 

                 846     202      77
 
BamHI G'GATC_C

Cuts at:      0     822    1125 
   Size:        822     303
 
BanI G'GyrC_C

Cuts at:      0     949    1125 
   Size:        949     176
 
BanII G_rGCy'C

Cuts at:      0     544    1125 
   Size:        544     581
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     634    1125 
   Size:        634     491
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       6      40    1125 
   Size:          6      34    1085

  Fragments arranged by size: 

                1085      34       6
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     187     381     482    1125 
   Size:        187     194     101     643

  Fragments arranged by size: 

                 643     194     187     101
 
BclI T'GATC_A

Cuts at:      0     960    1125 
   Size:        960     165
 
BlpI GC'TnA_GC

Cuts at:      0     799    1125 
   Size:        799     326
 
Bme1580I G_kGCm'C

Cuts at:      0      37    1125 
   Size:         37    1088
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     318     975    1125 
   Size:        318     657     150

  Fragments arranged by size: 

                 657     318     150
 
Bpu10I CC'TnA_GC

Cuts at:      0      92    1125 
   Size:         92    1033
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     318     439     661    1125 
   Size:        318     121     222     464

  Fragments arranged by size: 

                 464     318     222     121
 
BsaWI w'CCGG_w

Cuts at:      0      56    1125 
   Size:         56    1069
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     305     308    1125 
   Size:        305       3     817

  Fragments arranged by size: 

                 817     305       3
 
BsiEI CG_ry'CG

Cuts at:      0      61    1125 
   Size:         61    1064
 
BsiHKAI G_wGCw'C

Cuts at:      0     544    1125 
   Size:        544     581
 
BsmI GAATG_Cn'

Cuts at:      0     153     224     595    1125 
   Size:        153      71     371     530

  Fragments arranged by size: 

                 530     371     153      71
 
Bsp1286I G_dGCh'C

Cuts at:      0      37     544    1125 
   Size:         37     507     581

  Fragments arranged by size: 

                 581     507      37
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     400    1125 
   Size:        400     725
 
BsrFI r'CCGG_y

Cuts at:      0      56    1125 
   Size:         56    1069
 
BssSI C'ACGA_G

Cuts at:      0     881    1125 
   Size:        881     244
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     424    1125 
   Size:        424     701
 
BstYI r'GATC_y

Cuts at:      0     822    1125 
   Size:        822     303
 
Bsu36I CC'TnA_GG

Cuts at:      0     112    1125 
   Size:        112    1013
 
BtgI C'CryG_G

Cuts at:      0     658    1125 
   Size:        658     467
 
ClaI AT'CG_AT

Cuts at:      0     620    1125 
   Size:        620     505
 
DraIII CAC_nnn'GTG

Cuts at:      0     664    1125 
   Size:        664     461
 
EarI CTCTTCn'nnn_

Cuts at:      0      93     341    1125 
   Size:         93     248     784

  Fragments arranged by size: 

                 784     248      93
 
EcoICRI GAG'CTC

Cuts at:      0     542    1125 
   Size:        542     583
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     163     381    1125 
   Size:        149      14     218     744

  Fragments arranged by size: 

                 744     218     149      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     342     374    1125 
   Size:        342      32     751

  Fragments arranged by size: 

                 751     342      32
 
KpnI G_GTAC'C

Cuts at:      0     953    1125 
   Size:        953     172
 
MspA1I CmG'CkG

Cuts at:      0     174    1125 
   Size:        174     951
 
PasI CC'CwG_GG

Cuts at:      0     786    1125 
   Size:        786     339
 
PfoI T'CCnGG_A

Cuts at:      0     526     890    1125 
   Size:        526     364     235

  Fragments arranged by size: 

                 526     364     235
 
PstI C_TGCA'G

Cuts at:      0     480    1125 
   Size:        480     645
 
PvuII CAG'CTG

Cuts at:      0     174    1125 
   Size:        174     951
 
SacI G_AGCT'C

Cuts at:      0     544    1125 
   Size:        544     581
 
SfcI C'TryA_G

Cuts at:      0     476    1125 
   Size:        476     649
 
SmlI C'TyrA_G

Cuts at:      0     333     454     640    1125 
   Size:        333     121     186     485

  Fragments arranged by size: 

                 485     333     186     121
 
SpeI A'CTAG_T

Cuts at:      0    1039    1125 
   Size:       1039      86
 
TatI w'GTAC_w

Cuts at:      0     207    1125 
   Size:        207     918
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AgeI     ApoI    BamHI     BanI    BanII
     BbsI     BcgI    BciVI     BclI     BlpI Bme1580I     BmrI   Bpu10I
    BpuEI    BsaWI    BseRI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrFI    BssSI   BstAPI    BstYI   Bsu36I     BtgI     ClaI   DraIII
     EarI  EcoICRI  Eco57MI    Hin4I     KpnI   MspA1I     PasI     PfoI
     PstI    PvuII     SacI     SfcI     SmlI     SpeI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BbeI    BbvCI    BfrBI     BglI    BglII
    BmgBI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseYI     BsgI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I    BtgZI
     BtsI    CspCI     DraI     DrdI     EaeI     EagI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI