(Linear) (Six-base) MAPSORT of: KIEE3155.seq  Check: 3508  from: 1  to: 1119



KIEE3155.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:24  ..

AccI GT'mk_AC

Cuts at:      0     149    1119 
   Size:        149     970
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     984    1119 
   Size:        984     135
 
AlwNI CAG_nnn'CTG

Cuts at:      0     289     721    1119 
   Size:        289     432     398

  Fragments arranged by size: 

                 432     398     289
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     399     496    1119 
   Size:        399      97     623

  Fragments arranged by size: 

                 623     399      97
 
BglII A'GATC_T

Cuts at:      0     675    1119 
   Size:        675     444
 
BlpI GC'TnA_GC

Cuts at:      0     404    1119 
   Size:        404     715
 
BmtI G_CTAG'C

Cuts at:      0     215    1119 
   Size:        215     904
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    1119 
   Size:        571     548
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      70     100     842     872    1119 
   Size:         70      30     742      30     247

  Fragments arranged by size: 

                 742     247      70      30      30
 
BseYI C'CCAG_C

Cuts at:      0     422    1119 
   Size:        422     697
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259    1119 
   Size:        259     860
 
BsrGI T'GTAC_A

Cuts at:      0     812    1119 
   Size:        812     307
 
BssSI C'ACGA_G

Cuts at:      0     325    1119 
   Size:        325     794
 
BstYI r'GATC_y

Cuts at:      0     675    1109    1119 
   Size:        675     434      10

  Fragments arranged by size: 

                 675     434      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     384    1119 
   Size:        384     735
 
BtsI GCAGTG_nn'

Cuts at:      0     469    1119 
   Size:        469     650
 
EaeI y'GGCC_r

Cuts at:      0     112    1119 
   Size:        112    1007
 
EarI CTCTTCn'nnn_

Cuts at:      0     551    1119 
   Size:        551     568
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571    1119 
   Size:        571     548
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     328     360     468     500     647     679     819     842 
   Size:        328      32     108      32     147      32     140      23

Cuts at:    842     851     874    1119 
   Size:          9      23     245

  Fragments arranged by size: 

                 328     245     147     140     108      32      32      32
                  23      23       9
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     304    1119 
   Size:        304     815
 
MslI CAynn'nnrTG

Cuts at:      0     253     814    1119 
   Size:        253     561     305

  Fragments arranged by size: 

                 561     305     253
 
MspA1I CmG'CkG

Cuts at:      0     271     895    1119 
   Size:        271     624     224

  Fragments arranged by size: 

                 624     271     224
 
NheI G'CTAG_C

Cuts at:      0     211    1119 
   Size:        211     908
 
NspI r_CATG'y

Cuts at:      0     607    1119 
   Size:        607     512
 
PasI CC'CwG_GG

Cuts at:      0     570    1119 
   Size:        570     549
 
PshAI GACnn'nnGTC

Cuts at:      0    1052    1119 
   Size:       1052      67
 
PstI C_TGCA'G

Cuts at:      0     476    1119 
   Size:        476     643
 
PvuII CAG'CTG

Cuts at:      0     895    1119 
   Size:        895     224
 
SfcI C'TryA_G

Cuts at:      0     332     472     896    1064    1119 
   Size:        332     140     424     168      55

  Fragments arranged by size: 

                 424     332     168     140      55
 
SspI AAT'ATT

Cuts at:      0     693    1119 
   Size:        693     426
 
StyI C'CwwG_G

Cuts at:      0     104     206    1119 
   Size:        104     102     913

  Fragments arranged by size: 

                 913     104     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     503    1119 
   Size:        503     616
 
TatI w'GTAC_w

Cuts at:      0     812    1119 
   Size:        812     307
 

 Enzymes that do cut:

     AccI     AlfI    AlwNI     BbsI    BglII     BlpI     BmtI     BpmI
    BsaXI    BseYI     BsgI    BsrGI    BssSI    BstYI    BtgZI     BtsI
     EaeI     EarI  Eco57MI    Hin4I     MmeI     MslI   MspA1I     NheI
     NspI     PasI    PshAI     PstI    PvuII     SfcI     SspI     StyI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I
    BmgBI     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI