Restriction map for
pFN21AE3154
(Linear) (Six-base) MAPSORT of: KIEE3154.seq Check: 322 from: 1 to: 1116
KIEE3154.orf
With 182 enzymes: SgfI *
July 3, 2012 17:23 ..
AatII G_ACGT'C
Cuts at: 0 244 1116
Size: 244 872
ApaI G_GGCC'C
Cuts at: 0 435 669 1116
Size: 435 234 447
Fragments arranged by size:
447 435 234
AvaI C'yCGr_G
Cuts at: 0 335 423 532 662 787 984 1116
Size: 335 88 109 130 125 197 132
Fragments arranged by size:
335 197 132 130 125 109 88
BanI G'GyrC_C
Cuts at: 0 151 188 558 598 842 1116
Size: 151 37 370 40 244 274
Fragments arranged by size:
370 274 244 151 40 37
BanII G_rGCy'C
Cuts at: 0 146 435 612 669 1116
Size: 146 289 177 57 447
Fragments arranged by size:
447 289 177 146 57
BbeI G_GCGC'C
Cuts at: 0 192 602 1116
Size: 192 410 514
Fragments arranged by size:
514 410 192
BbsI GAAGACnn'nnnn_
Cuts at: 0 381 1116
Size: 381 735
BglI GCCn_nnn'nGGC
Cuts at: 0 607 791 1116
Size: 607 184 325
Fragments arranged by size:
607 325 184
BlpI GC'TnA_GC
Cuts at: 0 416 493 1116
Size: 416 77 623
Fragments arranged by size:
623 416 77
Bme1580I G_kGCm'C
Cuts at: 0 95 156 435 563 669 1016 1116
Size: 95 61 279 128 106 347 100
Fragments arranged by size:
347 279 128 106 100 95 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 1116
Size: 369 747
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 460 1116
Size: 103 357 656
Fragments arranged by size:
656 357 103
BsaI GGTCTCn'nnnn_
Cuts at: 0 211 1116
Size: 211 905
BsaAI yAC'GTr
Cuts at: 0 273 819 1116
Size: 273 546 297
Fragments arranged by size:
546 297 273
BsaHI Gr'CG_yC
Cuts at: 0 189 201 241 599 761 1116
Size: 189 12 40 358 162 355
Fragments arranged by size:
358 355 189 162 40 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 208 238 1116
Size: 208 30 878
Fragments arranged by size:
878 208 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 31 34 292 1116
Size: 28 3 3 258 824
Fragments arranged by size:
824 258 28 3 3
BseYI C'CCAG_C
Cuts at: 0 102 282 485 594 848 1116
Size: 102 180 203 109 254 268
Fragments arranged by size:
268 254 203 180 109 102
BsiEI CG_ry'CG
Cuts at: 0 169 359 751 980 1116
Size: 169 190 392 229 136
Fragments arranged by size:
392 229 190 169 136
BsiHKAI G_wGCw'C
Cuts at: 0 1043 1116
Size: 1043 73
Bsp1286I G_dGCh'C
Cuts at: 0 95 146 156 435 563 612 669 1016
Size: 95 51 10 279 128 49 57 347
Cuts at: 1016 1043 1116
Size: 27 73
Fragments arranged by size:
347 279 128 95 73 57 51 49
27 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1007 1116
Size: 1007 109
BsrDI GCAATG_nn'
Cuts at: 0 366 1116
Size: 366 750
BsrFI r'CCGG_y
Cuts at: 0 168 537 936 1116
Size: 168 369 399 180
Fragments arranged by size:
399 369 180 168
BssHII G'CGCG_C
Cuts at: 0 582 680 682 803 899 960 962 1018
Size: 582 98 2 121 96 61 2 56
Cuts at: 1018 1116
Size: 98
Fragments arranged by size:
582 121 98 98 96 61 56 2
2
BstEII G'GTnAC_C
Cuts at: 0 252 711 1116
Size: 252 459 405
Fragments arranged by size:
459 405 252
BtgI C'CryG_G
Cuts at: 0 321 359 751 1116
Size: 321 38 392 365
Fragments arranged by size:
392 365 321 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 152 840 1116
Size: 152 688 276
Fragments arranged by size:
688 276 152
DraIII CAC_nnn'GTG
Cuts at: 0 954 1116
Size: 954 162
EaeI y'GGCC_r
Cuts at: 0 27 166 356 625 689 748 875 977
Size: 27 139 190 269 64 59 127 102
Cuts at: 977 1116
Size: 139
Fragments arranged by size:
269 190 139 139 127 102 64 59
27
EagI C'GGCC_G
Cuts at: 0 166 356 748 977 1116
Size: 166 190 392 229 139
Fragments arranged by size:
392 229 190 166 139
EarI CTCTTCn'nnn_
Cuts at: 0 1048 1116
Size: 1048 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 538 628 1116
Size: 252 286 90 488
Fragments arranged by size:
488 286 252 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 460 1116
Size: 103 357 656
Fragments arranged by size:
656 357 103
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 691 1116
Size: 118 573 425
Fragments arranged by size:
573 425 118
EcoO109I rG'GnC_Cy
Cuts at: 0 19 207 666 1116
Size: 19 188 459 450
Fragments arranged by size:
459 450 188 19
HaeII r_GCGC'y
Cuts at: 0 192 423 602 649 655 797 943 1116
Size: 192 231 179 47 6 142 146 173
Fragments arranged by size:
231 192 179 173 146 142 47 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 1116
Size: 208 32 876
Fragments arranged by size:
876 208 32
KasI G'GCGC_C
Cuts at: 0 188 598 1116
Size: 188 410 518
Fragments arranged by size:
518 410 188
MscI TGG'CCA
Cuts at: 0 877 1116
Size: 877 239
MslI CAynn'nnrTG
Cuts at: 0 1036 1093 1116
Size: 1036 57 23
Fragments arranged by size:
1036 57 23
MspA1I CmG'CkG
Cuts at: 0 323 361 415 753 848 1116
Size: 323 38 54 338 95 268
Fragments arranged by size:
338 323 268 95 54 38
NaeI GCC'GGC
Cuts at: 0 938 1116
Size: 938 178
NarI GG'CG_CC
Cuts at: 0 189 599 1116
Size: 189 410 517
Fragments arranged by size:
517 410 189
NgoMIV G'CCGG_C
Cuts at: 0 936 1116
Size: 936 180
NotI GC'GGCC_GC
Cuts at: 0 977 1116
Size: 977 139
PasI CC'CwG_GG
Cuts at: 0 147 247 567 1116
Size: 147 100 320 549
Fragments arranged by size:
549 320 147 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 741 1116
Size: 741 375
PfoI T'CCnGG_A
Cuts at: 0 314 1116
Size: 314 802
PmlI CAC'GTG
Cuts at: 0 273 1116
Size: 273 843
PpuMI rG'GwC_Cy
Cuts at: 0 19 207 1116
Size: 19 188 909
Fragments arranged by size:
909 188 19
PshAI GACnn'nnGTC
Cuts at: 0 385 1116
Size: 385 731
PspOMI G'GGCC_C
Cuts at: 0 431 665 1116
Size: 431 234 451
Fragments arranged by size:
451 431 234
RsrII CG'GwC_CG
Cuts at: 0 318 1116
Size: 318 798
SacII CC_GC'GG
Cuts at: 0 324 362 754 1116
Size: 324 38 392 362
Fragments arranged by size:
392 362 324 38
SanDI GG'GwC_CC
Cuts at: 0 207 1116
Size: 207 909
SapI GCTCTTCn'nnn_
Cuts at: 0 1048 1116
Size: 1048 68
SexAI A'CCwGG_T
Cuts at: 0 220 1116
Size: 220 896
SfoI GGC'GCC
Cuts at: 0 190 600 1116
Size: 190 410 516
Fragments arranged by size:
516 410 190
SgrAI Cr'CCGG_yG
Cuts at: 0 936 1116
Size: 936 180
SmaI CCC'GGG
Cuts at: 0 337 534 664 789 986 1116
Size: 337 197 130 125 197 130
Fragments arranged by size:
337 197 197 130 130 125
StuI AGG'CCT
Cuts at: 0 697 1116
Size: 697 419
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 287 319 1116
Size: 287 32 797
Fragments arranged by size:
797 287 32
Tth111I GACn'n_nGTC
Cuts at: 0 222 1116
Size: 222 894
XmaI C'CCGG_G
Cuts at: 0 335 532 662 787 984 1116
Size: 335 197 130 125 197 132
Fragments arranged by size:
335 197 197 132 130 125
ZraI GAC'GTC
Cuts at: 0 242 1116
Size: 242 874
Enzymes that do cut:
AatII ApaI AvaI BanI BanII BbeI BbsI BglI
BlpI Bme1580I BplI BpmI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BssHII BstEII BtgI BtgZI DraIII EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI
MslI MspA1I NaeI NarI NgoMIV NotI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI RsrII SacII SanDI
SapI SexAI SfoI SgrAI SmaI StuI TstI Tth111I
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI Bpu10I
BpuEI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI
NruI NsiI NspI PacI PciI PmeI PpiI PsiI
PspXI PsrI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfiI SgfI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII TatI XbaI XcmI
XhoI XmnI