Restriction map for
pFN21AE3152
(Linear) (Six-base) MAPSORT of: KIEE3152.seq Check: 4757 from: 1 to: 1092
KIEE3152.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
Acc65I G'GTAC_C
Cuts at: 0 310 1092
Size: 310 782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 704 768 1046 1092
Size: 586 118 64 278 46
Fragments arranged by size:
586 278 118 64 46
AhdI GACnn_n'nnGTC
Cuts at: 0 242 1092
Size: 242 850
AleI CACnn'nnGTG
Cuts at: 0 109 1092
Size: 109 983
ApaI G_GGCC'C
Cuts at: 0 198 1035 1092
Size: 198 837 57
Fragments arranged by size:
837 198 57
ApaLI G'TGCA_C
Cuts at: 0 1015 1092
Size: 1015 77
ApoI r'AATT_y
Cuts at: 0 709 1092
Size: 709 383
AvaI C'yCGr_G
Cuts at: 0 65 148 229 323 1092
Size: 65 83 81 94 769
Fragments arranged by size:
769 94 83 81 65
BamHI G'GATC_C
Cuts at: 0 408 1092
Size: 408 684
BanI G'GyrC_C
Cuts at: 0 121 310 902 978 1092
Size: 121 189 592 76 114
Fragments arranged by size:
592 189 121 114 76
BanII G_rGCy'C
Cuts at: 0 198 965 1035 1092
Size: 198 767 70 57
Fragments arranged by size:
767 198 70 57
BbeI G_GCGC'C
Cuts at: 0 125 906 982 1092
Size: 125 781 76 110
Fragments arranged by size:
781 125 110 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 269 1092
Size: 269 823
BbvCI CC'TCA_GC
Cuts at: 0 511 1092
Size: 511 581
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 604 638 1092
Size: 604 34 454
Fragments arranged by size:
604 454 34
Bme1580I G_kGCm'C
Cuts at: 0 163 198 1019 1035 1092
Size: 163 35 821 16 57
Fragments arranged by size:
821 163 57 35 16
BmtI G_CTAG'C
Cuts at: 0 859 1092
Size: 859 233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 839 887 1092
Size: 239 600 48 205
Fragments arranged by size:
600 239 205 48
Bpu10I CC'TnA_GC
Cuts at: 0 511 916 1092
Size: 511 405 176
Fragments arranged by size:
511 405 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1092
Size: 652 440
BsaI GGTCTCn'nnnn_
Cuts at: 0 229 355 1092
Size: 229 126 737
Fragments arranged by size:
737 229 126
BsaBI GATnn'nnATC
Cuts at: 0 595 1092
Size: 595 497
BsaHI Gr'CG_yC
Cuts at: 0 122 226 903 979 1092
Size: 122 104 677 76 113
Fragments arranged by size:
677 122 113 104 76
BsaWI w'CCGG_w
Cuts at: 0 926 1092
Size: 926 166
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 528 1063 1092
Size: 528 535 29
Fragments arranged by size:
535 528 29
BseYI C'CCAG_C
Cuts at: 0 606 694 841 871 1034 1092
Size: 606 88 147 30 163 58
Fragments arranged by size:
606 163 147 88 58 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1092
Size: 724 368
BsiEI CG_ry'CG
Cuts at: 0 186 558 688 1092
Size: 186 372 130 404
Fragments arranged by size:
404 372 186 130
BsiHKAI G_wGCw'C
Cuts at: 0 965 1019 1092
Size: 965 54 73
Fragments arranged by size:
965 73 54
BsiWI C'GTAC_G
Cuts at: 0 240 1092
Size: 240 852
BsmI GAATG_Cn'
Cuts at: 0 819 1092
Size: 819 273
Bsp1286I G_dGCh'C
Cuts at: 0 163 198 965 1019 1035 1092
Size: 163 35 767 54 16 57
Fragments arranged by size:
767 163 57 54 35 16
BspEI T'CCGG_A
Cuts at: 0 926 1092
Size: 926 166
BsrFI r'CCGG_y
Cuts at: 0 118 185 557 971 1092
Size: 118 67 372 414 121
Fragments arranged by size:
414 372 121 118 67
BsrGI T'GTAC_A
Cuts at: 0 524 1092
Size: 524 568
BssHII G'CGCG_C
Cuts at: 0 436 438 1092
Size: 436 2 654
Fragments arranged by size:
654 436 2
BstYI r'GATC_y
Cuts at: 0 408 1092
Size: 408 684
BtgI C'CryG_G
Cuts at: 0 180 548 627 1092
Size: 180 368 79 465
Fragments arranged by size:
465 368 180 79
BtsI GCAGTG_nn'
Cuts at: 0 246 707 1092
Size: 246 461 385
Fragments arranged by size:
461 385 246
EaeI y'GGCC_r
Cuts at: 0 113 183 551 681 1092
Size: 113 70 368 130 411
Fragments arranged by size:
411 368 130 113 70
EagI C'GGCC_G
Cuts at: 0 183 1092
Size: 183 909
EarI CTCTTCn'nnn_
Cuts at: 0 288 334 419 1092
Size: 288 46 85 673
Fragments arranged by size:
673 288 85 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 221 399 1066 1092
Size: 221 178 667 26
Fragments arranged by size:
667 221 178 26
EcoICRI GAG'CTC
Cuts at: 0 963 1092
Size: 963 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 586 704 768 839 887 1046 1092
Size: 239 347 118 64 71 48 159 46
Fragments arranged by size:
347 239 159 118 71 64 48 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 206 484 1092
Size: 206 278 608
Fragments arranged by size:
608 278 206
EcoO109I rG'GnC_Cy
Cuts at: 0 194 394 741 1031 1092
Size: 194 200 347 290 61
Fragments arranged by size:
347 290 200 194 61
HaeII r_GCGC'y
Cuts at: 0 125 906 982 1092
Size: 125 781 76 110
Fragments arranged by size:
781 125 110 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 228 260 415 447 1036 1068 1092
Size: 228 32 155 32 589 32 24
Fragments arranged by size:
589 228 155 32 32 32 24
HincII GTy'rAC
Cuts at: 0 102 1092
Size: 102 990
KasI G'GCGC_C
Cuts at: 0 121 902 978 1092
Size: 121 781 76 114
Fragments arranged by size:
781 121 114 76
KpnI G_GTAC'C
Cuts at: 0 314 1092
Size: 314 778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1092
Size: 386 706
MscI TGG'CCA
Cuts at: 0 115 1092
Size: 115 977
MslI CAynn'nnrTG
Cuts at: 0 109 1092
Size: 109 983
MspA1I CmG'CkG
Cuts at: 0 182 465 564 606 1092
Size: 182 283 99 42 486
Fragments arranged by size:
486 283 182 99 42
NaeI GCC'GGC
Cuts at: 0 187 973 1092
Size: 187 786 119
Fragments arranged by size:
786 187 119
NarI GG'CG_CC
Cuts at: 0 122 903 979 1092
Size: 122 781 76 113
Fragments arranged by size:
781 122 113 76
NcoI C'CATG_G
Cuts at: 0 627 1092
Size: 627 465
NgoMIV G'CCGG_C
Cuts at: 0 185 971 1092
Size: 185 786 121
Fragments arranged by size:
786 185 121
NheI G'CTAG_C
Cuts at: 0 855 1092
Size: 855 237
PflMI CCAn_nnn'nTGG
Cuts at: 0 736 1041 1092
Size: 736 305 51
Fragments arranged by size:
736 305 51
PfoI T'CCnGG_A
Cuts at: 0 356 1092
Size: 356 736
PpuMI rG'GwC_Cy
Cuts at: 0 741 1092
Size: 741 351
PshAI GACnn'nnGTC
Cuts at: 0 661 1092
Size: 661 431
PspOMI G'GGCC_C
Cuts at: 0 194 1031 1092
Size: 194 837 61
Fragments arranged by size:
837 194 61
PvuII CAG'CTG
Cuts at: 0 465 564 606 1092
Size: 465 99 42 486
Fragments arranged by size:
486 465 99 42
SacI G_AGCT'C
Cuts at: 0 965 1092
Size: 965 127
SacII CC_GC'GG
Cuts at: 0 183 1092
Size: 183 909
ScaI AGT'ACT
Cuts at: 0 725 889 1092
Size: 725 164 203
Fragments arranged by size:
725 203 164
SfcI C'TryA_G
Cuts at: 0 651 1092
Size: 651 441
SfoI GGC'GCC
Cuts at: 0 123 904 980 1092
Size: 123 781 76 112
Fragments arranged by size:
781 123 112 76
SgrAI Cr'CCGG_yG
Cuts at: 0 118 1092
Size: 118 974
SmaI CCC'GGG
Cuts at: 0 67 150 231 325 1092
Size: 67 83 81 94 767
Fragments arranged by size:
767 94 83 81 67
SmlI C'TyrA_G
Cuts at: 0 667 1092
Size: 667 425
StuI AGG'CCT
Cuts at: 0 479 673 1092
Size: 479 194 419
Fragments arranged by size:
479 419 194
StyI C'CwwG_G
Cuts at: 0 163 627 1092
Size: 163 464 465
Fragments arranged by size:
465 464 163
TatI w'GTAC_w
Cuts at: 0 524 723 887 1092
Size: 524 199 164 205
Fragments arranged by size:
524 205 199 164
XmaI C'CCGG_G
Cuts at: 0 65 148 229 323 1092
Size: 65 83 81 94 769
Fragments arranged by size:
769 94 83 81 65
Enzymes that do cut:
Acc65I AcuI AhdI AleI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI Bme1580I
BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BsrFI BsrGI BssHII BstYI BtgI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI PflMI PfoI PpuMI
PshAI PspOMI PvuII SacI SacII ScaI SfcI SfoI
SgrAI SmaI SmlI StuI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AlfI AloI AlwNI AscI AseI AsiSI AvrII BaeI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BplI BsaAI BsaXI BsmBI BspHI BspMI BsrBI BsrDI
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI MfeI MluI NdeI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PsiI PspXI PsrI PstI PvuI RsrII
SalI SanDI SapI SbfI SexAI SfiI SgfI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmnI ZraI