Restriction map for
pFN21AE3151
(Linear) (Six-base) MAPSORT of: KIEE3151.seq Check: 47 from: 1 to: 1101
KIEE3151.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 409 480 799 1101
Size: 409 71 319 302
Fragments arranged by size:
409 319 302 71
AfeI AGC'GCT
Cuts at: 0 874 886 1101
Size: 874 12 215
Fragments arranged by size:
874 215 12
AlwNI CAG_nnn'CTG
Cuts at: 0 924 1101
Size: 924 177
ApaI G_GGCC'C
Cuts at: 0 274 1012 1101
Size: 274 738 89
Fragments arranged by size:
738 274 89
AscI GG'CGCG_CC
Cuts at: 0 1025 1101
Size: 1025 76
AvaI C'yCGr_G
Cuts at: 0 64 447 615 1101
Size: 64 383 168 486
Fragments arranged by size:
486 383 168 64
BamHI G'GATC_C
Cuts at: 0 327 1101
Size: 327 774
BanI G'GyrC_C
Cuts at: 0 81 120 346 366 495 504 1101
Size: 81 39 226 20 129 9 597
Fragments arranged by size:
597 226 129 81 39 20 9
BanII G_rGCy'C
Cuts at: 0 274 448 616 863 1012 1101
Size: 274 174 168 247 149 89
Fragments arranged by size:
274 247 174 168 149 89
BbeI G_GCGC'C
Cuts at: 0 85 124 499 1101
Size: 85 39 375 602
Fragments arranged by size:
602 375 85 39
BglI GCCn_nnn'nGGC
Cuts at: 0 34 57 1042 1101
Size: 34 23 985 59
Fragments arranged by size:
985 59 34 23
BglII A'GATC_T
Cuts at: 0 827 1101
Size: 827 274
BlpI GC'TnA_GC
Cuts at: 0 434 610 910 1101
Size: 434 176 300 191
Fragments arranged by size:
434 300 191 176
Bme1580I G_kGCm'C
Cuts at: 0 7 274 377 386 1012 1101
Size: 7 267 103 9 626 89
Fragments arranged by size:
626 267 103 89 9 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 586 826 958 1101
Size: 145 441 240 132 143
Fragments arranged by size:
441 240 145 143 132
BsaHI Gr'CG_yC
Cuts at: 0 82 121 496 541 757 1101
Size: 82 39 375 45 216 344
Fragments arranged by size:
375 344 216 82 45 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 814 844 1101
Size: 814 30 257
Fragments arranged by size:
814 257 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 82 1029 1101
Size: 21 61 947 72
Fragments arranged by size:
947 72 61 21
BseYI C'CCAG_C
Cuts at: 0 105 480 1101
Size: 105 375 621
Fragments arranged by size:
621 375 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1101
Size: 556 545
BsiEI CG_ry'CG
Cuts at: 0 169 431 764 1046 1067 1101
Size: 169 262 333 282 21 34
Fragments arranged by size:
333 282 262 169 34 21
BsiHKAI G_wGCw'C
Cuts at: 0 179 1101
Size: 179 922
Bsp1286I G_dGCh'C
Cuts at: 0 7 179 274 377 386 448 616 863
Size: 7 172 95 103 9 62 168 247
Cuts at: 863 1012 1101
Size: 149 89
Fragments arranged by size:
247 172 168 149 103 95 89 62
9 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 409 480 728 799 948 1101
Size: 409 71 248 71 149 153
Fragments arranged by size:
409 248 153 149 71 71
BsrBI CCG'CTC
Cuts at: 0 433 653 1019 1101
Size: 433 220 366 82
Fragments arranged by size:
433 366 220 82
BsrFI r'CCGG_y
Cuts at: 0 258 501 547 1101
Size: 258 243 46 554
Fragments arranged by size:
554 258 243 46
BssHII G'CGCG_C
Cuts at: 0 1025 1101
Size: 1025 76
BssSI C'ACGA_G
Cuts at: 0 853 1101
Size: 853 248
BstAPI GCAn_nnn'nTGC
Cuts at: 0 924 1101
Size: 924 177
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 1101
Size: 360 741
BstYI r'GATC_y
Cuts at: 0 327 827 1101
Size: 327 500 274
Fragments arranged by size:
500 327 274
BtgI C'CryG_G
Cuts at: 0 89 266 386 520 639 1101
Size: 89 177 120 134 119 462
Fragments arranged by size:
462 177 134 120 119 89
DrdI GACnn_nn'nnGTC
Cuts at: 0 283 1101
Size: 283 818
EaeI y'GGCC_r
Cuts at: 0 35 166 209 428 761 1043 1064 1101
Size: 35 131 43 219 333 282 21 37
Fragments arranged by size:
333 282 219 131 43 37 35 21
EagI C'GGCC_G
Cuts at: 0 166 428 761 1043 1064 1101
Size: 166 262 333 282 21 37
Fragments arranged by size:
333 282 262 166 37 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 207 455 1101
Size: 207 248 646
Fragments arranged by size:
646 248 207
EcoO109I rG'GnC_Cy
Cuts at: 0 271 1008 1101
Size: 271 737 93
Fragments arranged by size:
737 271 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 891 1101
Size: 891 210
HaeII r_GCGC'y
Cuts at: 0 85 106 124 190 204 499 876 888
Size: 85 21 18 66 14 295 377 12
Cuts at: 888 1101
Size: 213
Fragments arranged by size:
377 295 213 85 66 21 18 14
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 742 774 1101
Size: 397 32 313 32 327
Fragments arranged by size:
397 327 313 32 32
KasI G'GCGC_C
Cuts at: 0 81 120 495 1101
Size: 81 39 375 606
Fragments arranged by size:
606 375 81 39
MspA1I CmG'CkG
Cuts at: 0 91 388 522 641 906 1001 1058 1101
Size: 91 297 134 119 265 95 57 43
Fragments arranged by size:
297 265 134 119 95 91 57 43
NaeI GCC'GGC
Cuts at: 0 260 1101
Size: 260 841
NarI GG'CG_CC
Cuts at: 0 82 121 496 1101
Size: 82 39 375 605
Fragments arranged by size:
605 375 82 39
NgoMIV G'CCGG_C
Cuts at: 0 258 1101
Size: 258 843
NotI GC'GGCC_GC
Cuts at: 0 428 1101
Size: 428 673
PflMI CCAn_nnn'nTGG
Cuts at: 0 1079 1101
Size: 1079 22
PspOMI G'GGCC_C
Cuts at: 0 270 1008 1101
Size: 270 738 93
Fragments arranged by size:
738 270 93
PstI C_TGCA'G
Cuts at: 0 668 776 1101
Size: 668 108 325
Fragments arranged by size:
668 325 108
PvuII CAG'CTG
Cuts at: 0 1058 1101
Size: 1058 43
SacII CC_GC'GG
Cuts at: 0 92 389 523 642 1101
Size: 92 297 134 119 459
Fragments arranged by size:
459 297 134 119 92
SexAI A'CCwGG_T
Cuts at: 0 704 1101
Size: 704 397
SfcI C'TryA_G
Cuts at: 0 664 772 1101
Size: 664 108 329
Fragments arranged by size:
664 329 108
SfoI GGC'GCC
Cuts at: 0 83 122 497 1101
Size: 83 39 375 604
Fragments arranged by size:
604 375 83 39
SgrAI Cr'CCGG_yG
Cuts at: 0 501 547 1101
Size: 501 46 554
Fragments arranged by size:
554 501 46
SmaI CCC'GGG
Cuts at: 0 617 1101
Size: 617 484
SmlI C'TyrA_G
Cuts at: 0 160 601 841 973 1101
Size: 160 441 240 132 128
Fragments arranged by size:
441 240 160 132 128
SrfI GCCC'GGGC
Cuts at: 0 617 1101
Size: 617 484
StuI AGG'CCT
Cuts at: 0 685 1101
Size: 685 416
StyI C'CwwG_G
Cuts at: 0 38 1101
Size: 38 1063
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 267 1101
Size: 267 834
XmaI C'CCGG_G
Cuts at: 0 615 1101
Size: 615 486
Enzymes that do cut:
AarI AfeI AlwNI ApaI AscI AvaI BamHI BanI
BanII BbeI BglI BglII BlpI Bme1580I BpuEI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI
DrdI EaeI EagI EciI EcoO109I FalI HaeII Hin4I
KasI MspA1I NaeI NarI NgoMIV NotI PflMI PspOMI
PstI PvuII SacII SexAI SfcI SfoI SgrAI SmaI
SmlI SrfI StuI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI
AvrII BaeI BbsI BbvCI BcgI BciVI BclI BfrBI
BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EarI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI NcoI NdeI NheI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SfiI
SgfI SnaBI SpeI SphI SspI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI