Restriction map for
pFN21AE3143
(Linear) (Six-base) MAPSORT of: KIEE3143.seq Check: 2108 from: 1 to: 1059
KIEE3143.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 238 430 1059
Size: 238 192 629
Fragments arranged by size:
629 238 192
AflIII A'CryG_T
Cuts at: 0 406 641 1059
Size: 406 235 418
Fragments arranged by size:
418 406 235
AlwNI CAG_nnn'CTG
Cuts at: 0 798 1059
Size: 798 261
ApoI r'AATT_y
Cuts at: 0 994 1059
Size: 994 65
BamHI G'GATC_C
Cuts at: 0 446 1059
Size: 446 613
BanI G'GyrC_C
Cuts at: 0 414 1059
Size: 414 645
BbsI GAAGACnn'nnnn_
Cuts at: 0 477 895 1059
Size: 477 418 164
Fragments arranged by size:
477 418 164
BclI T'GATC_A
Cuts at: 0 245 1059
Size: 245 814
BlpI GC'TnA_GC
Cuts at: 0 625 715 1059
Size: 625 90 344
Fragments arranged by size:
625 344 90
Bme1580I G_kGCm'C
Cuts at: 0 419 1059
Size: 419 640
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 227 312 592 1059
Size: 100 127 85 280 467
Fragments arranged by size:
467 280 127 100 85
BsaAI yAC'GTr
Cuts at: 0 407 1059
Size: 407 652
BsaBI GATnn'nnATC
Cuts at: 0 32 1059
Size: 32 1027
BsaHI Gr'CG_yC
Cuts at: 0 950 1059
Size: 950 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 505 535 1059
Size: 505 30 524
Fragments arranged by size:
524 505 30
BseYI C'CCAG_C
Cuts at: 0 882 1059
Size: 882 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1059
Size: 571 488
BsiEI CG_ry'CG
Cuts at: 0 337 1059
Size: 337 722
BsiHKAI G_wGCw'C
Cuts at: 0 328 374 941 1059
Size: 328 46 567 118
Fragments arranged by size:
567 328 118 46
Bsp1286I G_dGCh'C
Cuts at: 0 328 374 419 941 1059
Size: 328 46 45 522 118
Fragments arranged by size:
522 328 118 46 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 238 293 430 1059
Size: 238 55 137 629
Fragments arranged by size:
629 238 137 55
BsrDI GCAATG_nn'
Cuts at: 0 472 1059
Size: 472 587
BssSI C'ACGA_G
Cuts at: 0 238 1059
Size: 238 821
BstXI CCAn_nnnn'nTGG
Cuts at: 0 99 531 774 1059
Size: 99 432 243 285
Fragments arranged by size:
432 285 243 99
BstYI r'GATC_y
Cuts at: 0 446 1059
Size: 446 613
BtgI C'CryG_G
Cuts at: 0 38 1059
Size: 38 1021
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 758 793 1059
Size: 758 35 266
Fragments arranged by size:
758 266 35
DraIII CAC_nnn'GTG
Cuts at: 0 243 264 1059
Size: 243 21 795
Fragments arranged by size:
795 243 21
EaeI y'GGCC_r
Cuts at: 0 334 1059
Size: 334 725
EagI C'GGCC_G
Cuts at: 0 334 1059
Size: 334 725
EarI CTCTTCn'nnn_
Cuts at: 0 614 980 1013 1019 1059
Size: 614 366 33 6 40
Fragments arranged by size:
614 366 40 33 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 227 312 592 1059
Size: 100 127 85 280 467
Fragments arranged by size:
467 280 127 100 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 22 1059
Size: 22 1037
EcoO109I rG'GnC_Cy
Cuts at: 0 729 1059
Size: 729 330
EcoRI G'AATT_C
Cuts at: 0 994 1059
Size: 994 65
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 735 1059
Size: 735 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 237 269 1012 1044 1059
Size: 27 210 32 743 32 15
Fragments arranged by size:
743 210 32 32 27 15
HindIII A'AGCT_T
Cuts at: 0 712 863 1059
Size: 712 151 196
Fragments arranged by size:
712 196 151
MslI CAynn'nnrTG
Cuts at: 0 377 529 1059
Size: 377 152 530
Fragments arranged by size:
530 377 152
MspA1I CmG'CkG
Cuts at: 0 60 221 339 570 687 756 882 1059
Size: 60 161 118 231 117 69 126 177
Fragments arranged by size:
231 177 161 126 118 117 69 60
NcoI C'CATG_G
Cuts at: 0 38 1059
Size: 38 1021
PflMI CCAn_nnn'nTGG
Cuts at: 0 798 1059
Size: 798 261
PfoI T'CCnGG_A
Cuts at: 0 23 656 1059
Size: 23 633 403
Fragments arranged by size:
633 403 23
PmlI CAC'GTG
Cuts at: 0 407 1059
Size: 407 652
PsiI TTA'TAA
Cuts at: 0 8 1059
Size: 8 1051
PstI C_TGCA'G
Cuts at: 0 278 344 686 1059
Size: 278 66 342 373
Fragments arranged by size:
373 342 278 66
PvuII CAG'CTG
Cuts at: 0 60 570 756 882 1059
Size: 60 510 186 126 177
Fragments arranged by size:
510 186 177 126 60
SapI GCTCTTCn'nnn_
Cuts at: 0 614 1059
Size: 614 445
SexAI A'CCwGG_T
Cuts at: 0 608 1059
Size: 608 451
SfcI C'TryA_G
Cuts at: 0 274 340 682 1059
Size: 274 66 342 377
Fragments arranged by size:
377 342 274 66
StuI AGG'CCT
Cuts at: 0 457 1059
Size: 457 602
StyI C'CwwG_G
Cuts at: 0 38 593 869 1059
Size: 38 555 276 190
Fragments arranged by size:
555 276 190 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 531 563 1059
Size: 531 32 496
Fragments arranged by size:
531 496 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 774 795 1059
Size: 45 729 21 264
Fragments arranged by size:
729 264 45 21
Enzymes that do cut:
AarI AflIII AlwNI ApoI BamHI BanI BbsI BclI
BlpI Bme1580I BpmI BsaAI BsaBI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstXI
BstYI BtgI CspCI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HindIII MslI MspA1I
NcoI PflMI PfoI PmlI PsiI PstI PvuII SapI
SexAI SfcI StuI StyI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanII BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaWI BseRI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI DraI DrdI
EciI EcoICRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
Tth111I XbaI XhoI XmaI XmnI ZraI