Restriction map for
pFN21AE3142
(Linear) (Six-base) MAPSORT of: KIEE3142.seq Check: 1303 from: 1 to: 1059
KIEE3142.orf
With 182 enzymes: SgfI *
March 22, 2012 16:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 470 1059
Size: 470 589
AccI GT'mk_AC
Cuts at: 0 910 1059
Size: 910 149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1059
Size: 676 383
AleI CACnn'nnGTG
Cuts at: 0 946 1059
Size: 946 113
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 381 413 691 723 1059
Size: 381 32 278 32 336
Fragments arranged by size:
381 336 278 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1044 1059
Size: 1044 15
ApaI G_GGCC'C
Cuts at: 0 372 528 1059
Size: 372 156 531
Fragments arranged by size:
531 372 156
AvrII C'CTAG_G
Cuts at: 0 804 1059
Size: 804 255
BamHI G'GATC_C
Cuts at: 0 429 1059
Size: 429 630
BanII G_rGCy'C
Cuts at: 0 372 528 584 813 1059
Size: 372 156 56 229 246
Fragments arranged by size:
372 246 229 156 56
BbvCI CC'TCA_GC
Cuts at: 0 627 1059
Size: 627 432
BciVI GTATCCnnnnn_n'
Cuts at: 0 727 740 1059
Size: 727 13 319
Fragments arranged by size:
727 319 13
Bme1580I G_kGCm'C
Cuts at: 0 151 372 528 1059
Size: 151 221 156 531
Fragments arranged by size:
531 221 156 151
BmrI ACTGGGnnnn_n'
Cuts at: 0 313 1059
Size: 313 746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 693 847 1059
Size: 295 398 154 212
Fragments arranged by size:
398 295 212 154
Bpu10I CC'TnA_GC
Cuts at: 0 448 627 1059
Size: 448 179 432
Fragments arranged by size:
448 432 179
BsaI GGTCTCn'nnnn_
Cuts at: 0 226 1059
Size: 226 833
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 449 479 691 721 829 845 859 875
Size: 449 30 212 30 108 16 14 16
Cuts at: 875 879 909 1018 1048 1059
Size: 4 30 109 30 11
Fragments arranged by size:
449 212 109 108 30 30 30 30
16 16 14 11 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 62 244 349 1059
Size: 10 52 182 105 710
Fragments arranged by size:
710 182 105 52 10
BseYI C'CCAG_C
Cuts at: 0 392 547 1059
Size: 392 155 512
Fragments arranged by size:
512 392 155
BsiHKAI G_wGCw'C
Cuts at: 0 584 1059
Size: 584 475
BsmBI CGTCTCn'nnnn_
Cuts at: 0 361 1059
Size: 361 698
Bsp1286I G_dGCh'C
Cuts at: 0 151 372 528 584 813 1059
Size: 151 221 156 56 229 246
Fragments arranged by size:
246 229 221 156 151 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 1059
Size: 470 589
BssSI C'ACGA_G
Cuts at: 0 22 770 1059
Size: 22 748 289
Fragments arranged by size:
748 289 22
BstXI CCAn_nnnn'nTGG
Cuts at: 0 717 948 1059
Size: 717 231 111
Fragments arranged by size:
717 231 111
BstYI r'GATC_y
Cuts at: 0 429 1059
Size: 429 630
BstZ17I GTA'TAC
Cuts at: 0 911 1059
Size: 911 148
Bsu36I CC'TnA_GG
Cuts at: 0 825 1059
Size: 825 234
BtsI GCAGTG_nn'
Cuts at: 0 266 620 1059
Size: 266 354 439
Fragments arranged by size:
439 354 266
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 917 952 1059
Size: 917 35 107
Fragments arranged by size:
917 107 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 91 121 353 1059
Size: 91 30 232 706
Fragments arranged by size:
706 232 91 30
EarI CTCTTCn'nnn_
Cuts at: 0 922 929 1016 1059
Size: 922 7 87 43
Fragments arranged by size:
922 87 43 7
EcoICRI GAG'CTC
Cuts at: 0 582 1059
Size: 582 477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 676 693 847 1059
Size: 295 381 17 154 212
Fragments arranged by size:
381 295 212 154 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 795 1059
Size: 795 264
EcoO109I rG'GnC_Cy
Cuts at: 0 307 368 369 525 713 800 1059
Size: 307 61 1 156 188 87 259
Fragments arranged by size:
307 259 188 156 87 61 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 405 437 675 707 808 829 840 861
Size: 405 32 238 32 101 21 11 21
Cuts at: 861 1059
Size: 198
Fragments arranged by size:
405 238 198 101 32 32 21 21
11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 480 587 1012 1059
Size: 480 107 425 47
Fragments arranged by size:
480 425 107 47
MslI CAynn'nnrTG
Cuts at: 0 946 1059
Size: 946 113
MspA1I CmG'CkG
Cuts at: 0 541 1059
Size: 541 518
PfoI T'CCnGG_A
Cuts at: 0 783 1059
Size: 783 276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 1059
Size: 691 32 336
Fragments arranged by size:
691 336 32
PpuMI rG'GwC_Cy
Cuts at: 0 307 713 800 1059
Size: 307 406 87 259
Fragments arranged by size:
406 307 259 87
PspOMI G'GGCC_C
Cuts at: 0 368 524 1059
Size: 368 156 535
Fragments arranged by size:
535 368 156
PstI C_TGCA'G
Cuts at: 0 377 1059
Size: 377 682
PvuII CAG'CTG
Cuts at: 0 541 1059
Size: 541 518
SacI G_AGCT'C
Cuts at: 0 584 1059
Size: 584 475
SanDI GG'GwC_CC
Cuts at: 0 307 1059
Size: 307 752
SapI GCTCTTCn'nnn_
Cuts at: 0 922 1059
Size: 922 137
SfcI C'TryA_G
Cuts at: 0 373 1059
Size: 373 686
StyI C'CwwG_G
Cuts at: 0 236 363 384 804 1059
Size: 236 127 21 420 255
Fragments arranged by size:
420 255 236 127 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 546 870 945 1045 1059
Size: 546 324 75 100 14
Fragments arranged by size:
546 324 100 75 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 845 877 1059
Size: 845 32 182
Fragments arranged by size:
845 182 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 898 1059
Size: 898 161
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApaI AvrII
BamHI BanII BbvCI BciVI Bme1580I BmrI BpmI Bpu10I
BsaI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI
BssSI BstXI BstYI BstZ17I Bsu36I BtsI CspCI DrdI
EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MmeI MslI
MspA1I PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SanDI SapI SfcI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaLI ApoI AscI AseI AsiSI AvaI BaeI
BanI BbeI BbsI BcgI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BtgI
BtgZI ClaI DraI DraIII EaeI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI