Restriction map for
pFN21AE3141
(Linear) (Six-base) MAPSORT of: KIEE3141.seq Check: 8020 from: 1 to: 1053
KIEE3141.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AleI CACnn'nnGTG
Cuts at: 0 550 679 1053
Size: 550 129 374
Fragments arranged by size:
550 374 129
AlwNI CAG_nnn'CTG
Cuts at: 0 921 1053
Size: 921 132
ApoI r'AATT_y
Cuts at: 0 649 930 1053
Size: 649 281 123
Fragments arranged by size:
649 281 123
BamHI G'GATC_C
Cuts at: 0 809 938 1053
Size: 809 129 115
Fragments arranged by size:
809 129 115
BciVI GTATCCnnnnn_n'
Cuts at: 0 946 1053
Size: 946 107
Bme1580I G_kGCm'C
Cuts at: 0 263 1053
Size: 263 790
BmrI ACTGGGnnnn_n'
Cuts at: 0 815 1053
Size: 815 238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 238 290 685 943 1053
Size: 46 192 52 395 258 110
Fragments arranged by size:
395 258 192 110 52 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1053
Size: 928 125
BsaI GGTCTCn'nnnn_
Cuts at: 0 661 1053
Size: 661 392
BsaAI yAC'GTr
Cuts at: 0 681 1053
Size: 681 372
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 940 1053
Size: 196 744 113
Fragments arranged by size:
744 196 113
BsiHKAI G_wGCw'C
Cuts at: 0 203 1053
Size: 203 850
Bsp1286I G_dGCh'C
Cuts at: 0 203 263 1053
Size: 203 60 790
Fragments arranged by size:
790 203 60
BsrDI GCAATG_nn'
Cuts at: 0 720 728 1053
Size: 720 8 325
Fragments arranged by size:
720 325 8
BsrFI r'CCGG_y
Cuts at: 0 181 1053
Size: 181 872
BstXI CCAn_nnnn'nTGG
Cuts at: 0 552 1053
Size: 552 501
BstYI r'GATC_y
Cuts at: 0 117 809 938 1053
Size: 117 692 129 115
Fragments arranged by size:
692 129 117 115
Bsu36I CC'TnA_GG
Cuts at: 0 643 1053
Size: 643 410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 453 488 1053
Size: 453 35 565
Fragments arranged by size:
565 453 35
EaeI y'GGCC_r
Cuts at: 0 368 698 725 1053
Size: 368 330 27 328
Fragments arranged by size:
368 330 328 27
EarI CTCTTCn'nnn_
Cuts at: 0 71 1024 1053
Size: 71 953 29
Fragments arranged by size:
953 71 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 238 290 685 943 1053
Size: 46 192 52 395 258 110
Fragments arranged by size:
395 258 192 110 52 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 823 1053
Size: 220 603 230
Fragments arranged by size:
603 230 220
EcoO109I rG'GnC_Cy
Cuts at: 0 481 1053
Size: 481 572
EcoRI G'AATT_C
Cuts at: 0 930 1053
Size: 930 123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 73 358 1053
Size: 73 285 695
Fragments arranged by size:
695 285 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 278 310 660 692 1053
Size: 278 32 350 32 361
Fragments arranged by size:
361 350 278 32 32
HincII GTy'rAC
Cuts at: 0 606 1053
Size: 606 447
HindIII A'AGCT_T
Cuts at: 0 362 1053
Size: 362 691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 661 1053
Size: 74 587 392
Fragments arranged by size:
587 392 74
MscI TGG'CCA
Cuts at: 0 370 727 1053
Size: 370 357 326
Fragments arranged by size:
370 357 326
MslI CAynn'nnrTG
Cuts at: 0 550 679 1053
Size: 550 129 374
Fragments arranged by size:
550 374 129
MspA1I CmG'CkG
Cuts at: 0 142 406 895 994 1053
Size: 142 264 489 99 59
Fragments arranged by size:
489 264 142 99 59
NspI r_CATG'y
Cuts at: 0 747 1053
Size: 747 306
PflMI CCAn_nnn'nTGG
Cuts at: 0 921 1053
Size: 921 132
PmlI CAC'GTG
Cuts at: 0 681 1053
Size: 681 372
PpuMI rG'GwC_Cy
Cuts at: 0 481 1053
Size: 481 572
PstI C_TGCA'G
Cuts at: 0 569 1053
Size: 569 484
PvuII CAG'CTG
Cuts at: 0 142 406 895 994 1053
Size: 142 264 489 99 59
Fragments arranged by size:
489 264 142 99 59
SexAI A'CCwGG_T
Cuts at: 0 1016 1053
Size: 1016 37
SfcI C'TryA_G
Cuts at: 0 311 565 896 1053
Size: 311 254 331 157
Fragments arranged by size:
331 311 254 157
SmlI C'TyrA_G
Cuts at: 0 943 1053
Size: 943 110
StyI C'CwwG_G
Cuts at: 0 411 1053
Size: 411 642
TatI w'GTAC_w
Cuts at: 0 417 1053
Size: 417 636
XbaI T'CTAG_A
Cuts at: 0 105 1053
Size: 105 948
XmnI GAAnn'nnTTC
Cuts at: 0 101 688 1053
Size: 101 587 365
Fragments arranged by size:
587 365 101
Enzymes that do cut:
AleI AlwNI ApoI BamHI BciVI Bme1580I BmrI BpmI
BpuEI BsaI BsaAI BseRI BsiHKAI Bsp1286I BsrDI BsrFI
BstXI BstYI Bsu36I CspCI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NspI PflMI PmlI PpuMI PstI PvuII
SexAI SfcI SmlI StyI TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EagI EciI EcoICRI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XcmI XhoI XmaI ZraI