Restriction map for
pFN21AE3140
(Linear) (Six-base) MAPSORT of: KIEE3140.seq Check: 6341 from: 1 to: 1050
KIEE3140.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AccI GT'mk_AC
Cuts at: 0 458 1050
Size: 458 592
AlwNI CAG_nnn'CTG
Cuts at: 0 764 1050
Size: 764 286
ApoI r'AATT_y
Cuts at: 0 342 1050
Size: 342 708
AvaI C'yCGr_G
Cuts at: 0 65 473 1050
Size: 65 408 577
Fragments arranged by size:
577 408 65
BamHI G'GATC_C
Cuts at: 0 401 1050
Size: 401 649
BanI G'GyrC_C
Cuts at: 0 319 1050
Size: 319 731
BanII G_rGCy'C
Cuts at: 0 74 474 1050
Size: 74 400 576
Fragments arranged by size:
576 400 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 802 851 1050
Size: 802 49 199
Fragments arranged by size:
802 199 49
BciVI GTATCCnnnnn_n'
Cuts at: 0 598 1050
Size: 598 452
BglI GCCn_nnn'nGGC
Cuts at: 0 155 307 1050
Size: 155 152 743
Fragments arranged by size:
743 155 152
BglII A'GATC_T
Cuts at: 0 395 1050
Size: 395 655
Bme1580I G_kGCm'C
Cuts at: 0 450 1050
Size: 450 600
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 447 1050
Size: 447 603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 592 1050
Size: 145 447 458
Fragments arranged by size:
458 447 145
Bpu10I CC'TnA_GC
Cuts at: 0 969 1050
Size: 969 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 271 848 1050
Size: 165 106 577 202
Fragments arranged by size:
577 202 165 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 665 1050
Size: 665 385
BsaBI GATnn'nnATC
Cuts at: 0 400 1050
Size: 400 650
BsaWI w'CCGG_w
Cuts at: 0 596 1050
Size: 596 454
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 889 1050
Size: 889 161
BseYI C'CCAG_C
Cuts at: 0 222 975 1050
Size: 222 753 75
Fragments arranged by size:
753 222 75
BsiHKAI G_wGCw'C
Cuts at: 0 747 1050
Size: 747 303
Bsp1286I G_dGCh'C
Cuts at: 0 74 450 474 747 1050
Size: 74 376 24 273 303
Fragments arranged by size:
376 303 273 74 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 449 808 1050
Size: 449 359 242
Fragments arranged by size:
449 359 242
BsrBI CCG'CTC
Cuts at: 0 55 1050
Size: 55 995
BsrDI GCAATG_nn'
Cuts at: 0 206 1050
Size: 206 844
BstBI TT'CG_AA
Cuts at: 0 261 1050
Size: 261 789
BstXI CCAn_nnnn'nTGG
Cuts at: 0 310 699 1050
Size: 310 389 351
Fragments arranged by size:
389 351 310
BstYI r'GATC_y
Cuts at: 0 395 401 1008 1050
Size: 395 6 607 42
Fragments arranged by size:
607 395 42 6
BtgI C'CryG_G
Cuts at: 0 560 1050
Size: 560 490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 942 977 1050
Size: 942 35 73
Fragments arranged by size:
942 73 35
DraIII CAC_nnn'GTG
Cuts at: 0 339 1050
Size: 339 711
DrdI GACnn_nn'nnGTC
Cuts at: 0 289 1050
Size: 289 761
EaeI y'GGCC_r
Cuts at: 0 35 557 1050
Size: 35 522 493
Fragments arranged by size:
522 493 35
EarI CTCTTCn'nnn_
Cuts at: 0 953 1035 1050
Size: 953 82 15
Fragments arranged by size:
953 82 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 592 1050
Size: 145 447 458
Fragments arranged by size:
458 447 145
EcoNI CCTnn'n_nnAGG
Cuts at: 0 175 530 915 1050
Size: 175 355 385 135
Fragments arranged by size:
385 355 175 135
EcoO109I rG'GnC_Cy
Cuts at: 0 156 291 832 920 1050
Size: 156 135 541 88 130
Fragments arranged by size:
541 156 135 130 88
EcoRI G'AATT_C
Cuts at: 0 342 1050
Size: 342 708
MscI TGG'CCA
Cuts at: 0 559 1050
Size: 559 491
MspA1I CmG'CkG
Cuts at: 0 40 325 612 764 1050
Size: 40 285 287 152 286
Fragments arranged by size:
287 286 285 152 40
NcoI C'CATG_G
Cuts at: 0 560 1050
Size: 560 490
PpuMI rG'GwC_Cy
Cuts at: 0 291 832 920 1050
Size: 291 541 88 130
Fragments arranged by size:
541 291 130 88
PstI C_TGCA'G
Cuts at: 0 12 202 1050
Size: 12 190 848
Fragments arranged by size:
848 190 12
SfcI C'TryA_G
Cuts at: 0 8 198 326 1050
Size: 8 190 128 724
Fragments arranged by size:
724 190 128 8
SmaI CCC'GGG
Cuts at: 0 67 1050
Size: 67 983
SmlI C'TyrA_G
Cuts at: 0 144 286 863 1050
Size: 144 142 577 187
Fragments arranged by size:
577 187 144 142
StyI C'CwwG_G
Cuts at: 0 506 560 895 1050
Size: 506 54 335 155
Fragments arranged by size:
506 335 155 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 200 1050
Size: 200 850
TatI w'GTAC_w
Cuts at: 0 330 1050
Size: 330 720
XmaI C'CCGG_G
Cuts at: 0 65 1050
Size: 65 985
Enzymes that do cut:
AccI AlwNI ApoI AvaI BamHI BanI BanII BbsI
BciVI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BstBI BstXI BstYI BtgI CspCI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI MscI
MspA1I NcoI PpuMI PstI SfcI SmaI SmlI StyI
TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BclI
BfrBI BlpI BmgBI BmrI BmtI BsaAI BsaHI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI DraI EagI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI