Restriction map for
pFN21AE3138
(Linear) (Six-base) MAPSORT of: KIEE3138.seq Check: 9308 from: 1 to: 1050
KIEE3138.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AccI GT'mk_AC
Cuts at: 0 1010 1050
Size: 1010 40
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1050
Size: 154 896
AflIII A'CryG_T
Cuts at: 0 119 1050
Size: 119 931
AhdI GACnn_n'nnGTC
Cuts at: 0 39 1050
Size: 39 1011
ApaLI G'TGCA_C
Cuts at: 0 327 1050
Size: 327 723
ApoI r'AATT_y
Cuts at: 0 430 1050
Size: 430 620
AvaI C'yCGr_G
Cuts at: 0 49 671 1050
Size: 49 622 379
Fragments arranged by size:
622 379 49
BanII G_rGCy'C
Cuts at: 0 620 1050
Size: 620 430
BbsI GAAGACnn'nnnn_
Cuts at: 0 407 1050
Size: 407 643
BbvCI CC'TCA_GC
Cuts at: 0 821 1050
Size: 821 229
BclI T'GATC_A
Cuts at: 0 225 1050
Size: 225 825
Bme1580I G_kGCm'C
Cuts at: 0 331 1050
Size: 331 719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 435 1050
Size: 435 615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1022 1050
Size: 576 446 28
Fragments arranged by size:
576 446 28
Bpu10I CC'TnA_GC
Cuts at: 0 821 1050
Size: 821 229
BsaWI w'CCGG_w
Cuts at: 0 305 1050
Size: 305 745
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 76 1050
Size: 73 3 974
Fragments arranged by size:
974 73 3
BseYI C'CCAG_C
Cuts at: 0 83 1050
Size: 83 967
BsiHKAI G_wGCw'C
Cuts at: 0 331 620 1050
Size: 331 289 430
Fragments arranged by size:
430 331 289
BsmBI CGTCTCn'nnnn_
Cuts at: 0 15 985 1050
Size: 15 970 65
Fragments arranged by size:
970 65 15
Bsp1286I G_dGCh'C
Cuts at: 0 331 620 1050
Size: 331 289 430
Fragments arranged by size:
430 331 289
BspEI T'CCGG_A
Cuts at: 0 305 1050
Size: 305 745
BspHI T'CATG_A
Cuts at: 0 522 1050
Size: 522 528
BsrBI CCG'CTC
Cuts at: 0 66 1050
Size: 66 984
BstAPI GCAn_nnn'nTGC
Cuts at: 0 320 1050
Size: 320 730
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 710 1050
Size: 710 340
EaeI y'GGCC_r
Cuts at: 0 67 1050
Size: 67 983
EarI CTCTTCn'nnn_
Cuts at: 0 608 682 875 1050
Size: 608 74 193 175
Fragments arranged by size:
608 193 175 74
EcoICRI GAG'CTC
Cuts at: 0 618 1050
Size: 618 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 576 1022 1050
Size: 154 422 446 28
Fragments arranged by size:
446 422 154 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 539 1050
Size: 539 511
EcoO109I rG'GnC_Cy
Cuts at: 0 280 883 1050
Size: 280 603 167
Fragments arranged by size:
603 280 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 982 1014 1050
Size: 25 32 925 32 36
Fragments arranged by size:
925 36 32 32 25
HincII GTy'rAC
Cuts at: 0 934 1050
Size: 934 116
MspA1I CmG'CkG
Cuts at: 0 83 1050
Size: 83 967
NspI r_CATG'y
Cuts at: 0 123 1050
Size: 123 927
PciI A'CATG_T
Cuts at: 0 119 1050
Size: 119 931
PpuMI rG'GwC_Cy
Cuts at: 0 280 883 1050
Size: 280 603 167
Fragments arranged by size:
603 280 167
PvuII CAG'CTG
Cuts at: 0 83 1050
Size: 83 967
SacI G_AGCT'C
Cuts at: 0 620 1050
Size: 620 430
SanDI GG'GwC_CC
Cuts at: 0 280 1050
Size: 280 770
SapI GCTCTTCn'nnn_
Cuts at: 0 608 682 1050
Size: 608 74 368
Fragments arranged by size:
608 368 74
SfcI C'TryA_G
Cuts at: 0 236 920 1050
Size: 236 684 130
Fragments arranged by size:
684 236 130
SmaI CCC'GGG
Cuts at: 0 673 1050
Size: 673 377
StyI C'CwwG_G
Cuts at: 0 896 1050
Size: 896 154
TatI w'GTAC_w
Cuts at: 0 77 256 1050
Size: 77 179 794
Fragments arranged by size:
794 179 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 845 877 1050
Size: 845 32 173
Fragments arranged by size:
845 173 32
XmaI C'CCGG_G
Cuts at: 0 671 1050
Size: 671 379
Enzymes that do cut:
AccI AcuI AflIII AhdI ApaLI ApoI AvaI BanII
BbsI BbvCI BclI Bme1580I BplI BpmI Bpu10I BsaWI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrBI
BstAPI BtgZI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I HincII MspA1I NspI PciI PpuMI PvuII SacI
SanDI SapI SfcI SmaI StyI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XcmI XhoI XmnI ZraI