(Linear) (Six-base) MAPSORT of: KIEE3134.seq  Check: 6337  from: 1  to: 1020



KIEE3134.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:33  ..

AccI GT'mk_AC

Cuts at:      0     782    1020 
   Size:        782     238
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287    1020 
   Size:        287     733
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     906    1020 
   Size:        906     114
 
ApaI G_GGCC'C

Cuts at:      0     973    1020 
   Size:        973      47
 
ApoI r'AATT_y

Cuts at:      0     711     833    1020 
   Size:        711     122     187

  Fragments arranged by size: 

                 711     187     122
 
BanII G_rGCy'C

Cuts at:      0     938     973    1020 
   Size:        938      35      47

  Fragments arranged by size: 

                 938      47      35
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     767    1020 
   Size:        767     253
 
BclI T'GATC_A

Cuts at:      0     824    1020 
   Size:        824     196
 
Bme1580I G_kGCm'C

Cuts at:      0     973    1020 
   Size:        973      47
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     361    1020 
   Size:        361     659
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     446     788    1020 
   Size:        446     342     232

  Fragments arranged by size: 

                 446     342     232
 
Bpu10I CC'TnA_GC

Cuts at:      0     277     511    1020 
   Size:        277     234     509

  Fragments arranged by size: 

                 509     277     234
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     897     927    1020 
   Size:        897      30      93

  Fragments arranged by size: 

                 897      93      30
 
BseYI C'CCAG_C

Cuts at:      0       8     599    1020 
   Size:          8     591     421

  Fragments arranged by size: 

                 591     421       8
 
BsiHKAI G_wGCw'C

Cuts at:      0    1009    1020 
   Size:       1009      11
 
Bsp1286I G_dGCh'C

Cuts at:      0     938     973    1009    1020 
   Size:        938      35      36      11

  Fragments arranged by size: 

                 938      36      35      11
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     146     931    1020 
   Size:        146     785      89

  Fragments arranged by size: 

                 785     146      89
 
BstYI r'GATC_y

Cuts at:      0     238     408    1020 
   Size:        238     170     612

  Fragments arranged by size: 

                 612     238     170
 
BstZ17I GTA'TAC

Cuts at:      0     783    1020 
   Size:        783     237
 
BtgI C'CryG_G

Cuts at:      0     846    1020 
   Size:        846     174
 
BtsI GCAGTG_nn'

Cuts at:      0     457    1020 
   Size:        457     563
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     527     562    1020 
   Size:        527      35     458

  Fragments arranged by size: 

                 527     458      35
 
DraI TTT'AAA

Cuts at:      0     839    1020 
   Size:        839     181
 
EaeI y'GGCC_r

Cuts at:      0     921    1020 
   Size:        921      99
 
EarI CTCTTCn'nnn_

Cuts at:      0     365     725    1020 
   Size:        365     360     295

  Fragments arranged by size: 

                 365     360     295
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     446     788    1020 
   Size:        287     159     342     232

  Fragments arranged by size: 

                 342     287     232     159
 
EcoO109I rG'GnC_Cy

Cuts at:      0     969    1020 
   Size:        969      51
 
HindIII A'AGCT_T

Cuts at:      0      34    1020 
   Size:         34     986
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     503    1020 
   Size:         44     459     517

  Fragments arranged by size: 

                 517     459      44
 
MscI TGG'CCA

Cuts at:      0     923    1020 
   Size:        923      97
 
MslI CAynn'nnrTG

Cuts at:      0     479     862    1020 
   Size:        479     383     158

  Fragments arranged by size: 

                 479     383     158
 
MspA1I CmG'CkG

Cuts at:      0      12    1020 
   Size:         12    1008
 
NspI r_CATG'y

Cuts at:      0     794    1020 
   Size:        794     226
 
PfoI T'CCnGG_A

Cuts at:      0     423     765    1020 
   Size:        423     342     255

  Fragments arranged by size: 

                 423     342     255
 
PspOMI G'GGCC_C

Cuts at:      0     969    1020 
   Size:        969      51
 
PstI C_TGCA'G

Cuts at:      0     452    1020 
   Size:        452     568
 
PvuII CAG'CTG

Cuts at:      0      12    1020 
   Size:         12    1008
 
SfcI C'TryA_G

Cuts at:      0     448     690     724    1020 
   Size:        448     242      34     296

  Fragments arranged by size: 

                 448     296     242      34
 
SspI AAT'ATT

Cuts at:      0     337    1020 
   Size:        337     683
 
StuI AGG'CCT

Cuts at:      0     757    1020 
   Size:        757     263
 
StyI C'CwwG_G

Cuts at:      0     803    1020 
   Size:        803     217
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     622     654     897     929    1020 
   Size:        622      32     243      32      91

  Fragments arranged by size: 

                 622     243      91      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     300    1020 
   Size:        300     720
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     ApaI     ApoI    BanII    BciVI     BclI
 Bme1580I     BmrI     BpmI   Bpu10I    BsaXI    BseYI  BsiHKAI Bsp1286I
    BstXI    BstYI  BstZ17I     BtgI     BtsI    CspCI     DraI     EaeI
     EarI  Eco57MI EcoO109I  HindIII     MmeI     MscI     MslI   MspA1I
     NspI     PfoI   PspOMI     PstI    PvuII     SfcI     SspI     StuI
     StyI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
   Bsu36I    BtgZI     ClaI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI