(Linear) (Six-base) MAPSORT of: KIEE3133.seq  Check: 9582  from: 1  to: 1017



KIEE3133.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601    1017 
   Size:        601     416
 
AflIII A'CryG_T

Cuts at:      0     270    1017 
   Size:        270     747
 
AhdI GACnn_n'nnGTC

Cuts at:      0     876    1017 
   Size:        876     141
 
AleI CACnn'nnGTG

Cuts at:      0     521    1017 
   Size:        521     496
 
ApoI r'AATT_y

Cuts at:      0     705    1017 
   Size:        705     312
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     548    1017 
   Size:        548     469
 
BclI T'GATC_A

Cuts at:      0     327    1017 
   Size:        327     690
 
BglI GCCn_nnn'nGGC

Cuts at:      0     440    1017 
   Size:        440     577
 
Bme1580I G_kGCm'C

Cuts at:      0     405    1017 
   Size:        405     612
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     161     191     608     638    1017 
   Size:        161      30     417      30     379

  Fragments arranged by size: 

                 417     379     161      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91     937    1017 
   Size:         91     846      80

  Fragments arranged by size: 

                 846      91      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436    1017 
   Size:        436     581
 
BsiHKAI G_wGCw'C

Cuts at:      0     518    1017 
   Size:        518     499
 
Bsp1286I G_dGCh'C

Cuts at:      0     405     518    1017 
   Size:        405     113     499

  Fragments arranged by size: 

                 499     405     113
 
BspHI T'CATG_A

Cuts at:      0     867    1017 
   Size:        867     150
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     414    1017 
   Size:        414     603
 
BstBI TT'CG_AA

Cuts at:      0     276    1017 
   Size:        276     741
 
BtgI C'CryG_G

Cuts at:      0     435    1017 
   Size:        435     582
 
BtsI GCAGTG_nn'

Cuts at:      0     403    1017 
   Size:        403     614
 
EarI CTCTTCn'nnn_

Cuts at:      0     158     273    1017 
   Size:        158     115     744

  Fragments arranged by size: 

                 744     158     115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601    1017 
   Size:        601     416
 
EcoRV GAT'ATC

Cuts at:      0     498    1017 
   Size:        498     519
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     708     740     862     894    1017 
   Size:        708      32     122      32     123

  Fragments arranged by size: 

                 708     123     122      32      32
 
HincII GTy'rAC

Cuts at:      0      45     486    1017 
   Size:         45     441     531

  Fragments arranged by size: 

                 531     441      45
 
MslI CAynn'nnrTG

Cuts at:      0     521    1017 
   Size:        521     496
 
MspA1I CmG'CkG

Cuts at:      0     447    1017 
   Size:        447     570
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     272     304    1017 
   Size:        272      32     713

  Fragments arranged by size: 

                 713     272      32
 
PvuII CAG'CTG

Cuts at:      0     447    1017 
   Size:        447     570
 
SapI GCTCTTCn'nnn_

Cuts at:      0     158    1017 
   Size:        158     859
 
SfcI C'TryA_G

Cuts at:      0     318     519     995    1017 
   Size:        318     201     476      22

  Fragments arranged by size: 

                 476     318     201      22
 
StuI AGG'CCT

Cuts at:      0     604    1017 
   Size:        604     413
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     695     727    1017 
   Size:        695      32     290

  Fragments arranged by size: 

                 695     290      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     230    1017 
   Size:        230     787
 
XmnI GAAnn'nnTTC

Cuts at:      0     761    1017 
   Size:        761     256
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AleI     ApoI     BbsI     BclI     BglI
 Bme1580I    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI   BstAPI
    BstBI     BtgI     BtsI     EarI  Eco57MI    EcoRV    Hin4I   HincII
     MslI   MspA1I     PpiI    PvuII     SapI     SfcI     StuI     TstI
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TatI     XbaI     XcmI     XhoI
     XmaI     ZraI