Restriction map for
pFN21AE3129
(Linear) (Six-base) MAPSORT of: KIEE3129.seq Check: 1178 from: 1 to: 966
KIEE3129.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AatII G_ACGT'C
Cuts at: 0 208 966
Size: 208 758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 966
Size: 734 232
AfeI AGC'GCT
Cuts at: 0 639 966
Size: 639 327
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 547 579 966
Size: 547 32 387
Fragments arranged by size:
547 387 32
AlwNI CAG_nnn'CTG
Cuts at: 0 322 538 649 745 966
Size: 322 216 111 96 221
Fragments arranged by size:
322 221 216 111 96
ApoI r'AATT_y
Cuts at: 0 286 381 966
Size: 286 95 585
Fragments arranged by size:
585 286 95
BamHI G'GATC_C
Cuts at: 0 555 966
Size: 555 411
BanI G'GyrC_C
Cuts at: 0 163 966
Size: 163 803
BanII G_rGCy'C
Cuts at: 0 67 822 966
Size: 67 755 144
Fragments arranged by size:
755 144 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 869 966
Size: 869 97
BciVI GTATCCnnnnn_n'
Cuts at: 0 780 966
Size: 780 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 555 966
Size: 555 411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 395 452 966
Size: 134 261 57 514
Fragments arranged by size:
514 261 134 57
Bpu10I CC'TnA_GC
Cuts at: 0 746 966
Size: 746 220
BsaHI Gr'CG_yC
Cuts at: 0 7 205 966
Size: 7 198 761
Fragments arranged by size:
761 198 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 966
Size: 52 914
BseYI C'CCAG_C
Cuts at: 0 649 966
Size: 649 317
BsiHKAI G_wGCw'C
Cuts at: 0 671 822 966
Size: 671 151 144
Fragments arranged by size:
671 151 144
Bsp1286I G_dGCh'C
Cuts at: 0 67 671 822 966
Size: 67 604 151 144
Fragments arranged by size:
604 151 144 67
BsrDI GCAATG_nn'
Cuts at: 0 206 249 542 600 887 966
Size: 206 43 293 58 287 79
Fragments arranged by size:
293 287 206 79 58 43
BstXI CCAn_nnnn'nTGG
Cuts at: 0 444 966
Size: 444 522
BstYI r'GATC_y
Cuts at: 0 402 555 877 966
Size: 402 153 322 89
Fragments arranged by size:
402 322 153 89
Bsu36I CC'TnA_GG
Cuts at: 0 705 966
Size: 705 261
BtgI C'CryG_G
Cuts at: 0 126 209 791 966
Size: 126 83 582 175
Fragments arranged by size:
582 175 126 83
BtsI GCAGTG_nn'
Cuts at: 0 34 966
Size: 34 932
EcoICRI GAG'CTC
Cuts at: 0 820 966
Size: 820 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 395 452 734 966
Size: 134 261 57 282 232
Fragments arranged by size:
282 261 232 134 57
HaeII r_GCGC'y
Cuts at: 0 641 966
Size: 641 325
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 107 139 425 457 966
Size: 107 32 286 32 509
Fragments arranged by size:
509 286 107 32 32
NcoI C'CATG_G
Cuts at: 0 126 209 966
Size: 126 83 757
Fragments arranged by size:
757 126 83
NspI r_CATG'y
Cuts at: 0 870 966
Size: 870 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 112 966
Size: 112 854
PstI C_TGCA'G
Cuts at: 0 645 915 966
Size: 645 270 51
Fragments arranged by size:
645 270 51
SacI G_AGCT'C
Cuts at: 0 822 966
Size: 822 144
SexAI A'CCwGG_T
Cuts at: 0 80 966
Size: 80 886
SfcI C'TryA_G
Cuts at: 0 281 314 641 911 966
Size: 281 33 327 270 55
Fragments arranged by size:
327 281 270 55 33
StyI C'CwwG_G
Cuts at: 0 126 209 857 966
Size: 126 83 648 109
Fragments arranged by size:
648 126 109 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 91 966
Size: 91 875
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 710 742 966
Size: 710 32 224
Fragments arranged by size:
710 224 32
Tth111I GACn'n_nGTC
Cuts at: 0 82 966
Size: 82 884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 549 966
Size: 549 417
XmnI GAAnn'nnTTC
Cuts at: 0 324 966
Size: 324 642
ZraI GAC'GTC
Cuts at: 0 206 966
Size: 206 760
Enzymes that do cut:
AatII AcuI AfeI AloI AlwNI ApoI BamHI BanI
BanII BbsI BciVI BmrI BpmI Bpu10I BsaHI BseRI
BseYI BsiHKAI Bsp1286I BsrDI BstXI BstYI Bsu36I BtgI
BtsI EcoICRI Eco57MI HaeII Hin4I NcoI NspI PflMI
PstI SacI SexAI SfcI StyI TaqII TstI Tth111I
XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI XbaI XhoI
XmaI