Restriction map for
pFN21AE3126
(Linear) (Six-base) MAPSORT of: KIEE3126.seq Check: 4932 from: 1 to: 954
KIEE3126.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 736 954
Size: 736 218
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 954
Size: 617 337
AfeI AGC'GCT
Cuts at: 0 140 656 759 954
Size: 140 516 103 195
Fragments arranged by size:
516 195 140 103
AlwNI CAG_nnn'CTG
Cuts at: 0 288 954
Size: 288 666
ApaI G_GGCC'C
Cuts at: 0 28 115 954
Size: 28 87 839
Fragments arranged by size:
839 87 28
AvaI C'yCGr_G
Cuts at: 0 72 169 195 231 618 666 715 787
Size: 72 97 26 36 387 48 49 72
Cuts at: 787 954
Size: 167
Fragments arranged by size:
387 167 97 72 72 49 48 36
26
BanI G'GyrC_C
Cuts at: 0 226 247 486 648 778 954
Size: 226 21 239 162 130 176
Fragments arranged by size:
239 226 176 162 130 21
BanII G_rGCy'C
Cuts at: 0 28 115 210 665 954
Size: 28 87 95 455 289
Fragments arranged by size:
455 289 95 87 28
BbeI G_GCGC'C
Cuts at: 0 230 251 652 954
Size: 230 21 401 302
Fragments arranged by size:
401 302 230 21
BbvCI CC'TCA_GC
Cuts at: 0 292 954
Size: 292 662
BglI GCCn_nnn'nGGC
Cuts at: 0 245 275 607 800 954
Size: 245 30 332 193 154
Fragments arranged by size:
332 245 193 154 30
BglII A'GATC_T
Cuts at: 0 380 954
Size: 380 574
BlpI GC'TnA_GC
Cuts at: 0 142 265 373 954
Size: 142 123 108 581
Fragments arranged by size:
581 142 123 108
Bme1580I G_kGCm'C
Cuts at: 0 28 115 491 954
Size: 28 87 376 463
Fragments arranged by size:
463 376 87 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 954
Size: 679 275
Bpu10I CC'TnA_GC
Cuts at: 0 292 954
Size: 292 662
BsaAI yAC'GTr
Cuts at: 0 393 954
Size: 393 561
BsaHI Gr'CG_yC
Cuts at: 0 227 248 649 954
Size: 227 21 401 305
Fragments arranged by size:
401 305 227 21
BsaWI w'CCGG_w
Cuts at: 0 736 954
Size: 736 218
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 831 861 954
Size: 831 30 93
Fragments arranged by size:
831 93 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 211 603 916 954
Size: 21 190 392 313 38
Fragments arranged by size:
392 313 190 38 21
BsiEI CG_ry'CG
Cuts at: 0 307 611 902 954
Size: 307 304 291 52
Fragments arranged by size:
307 304 291 52
Bsp1286I G_dGCh'C
Cuts at: 0 28 115 210 491 665 954
Size: 28 87 95 281 174 289
Fragments arranged by size:
289 281 174 95 87 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 695 736 954
Size: 695 41 218
Fragments arranged by size:
695 218 41
BsrBI CCG'CTC
Cuts at: 0 219 954
Size: 219 735
BsrFI r'CCGG_y
Cuts at: 0 904 954
Size: 904 50
BssHII G'CGCG_C
Cuts at: 0 350 954
Size: 350 604
BstAPI GCAn_nnn'nTGC
Cuts at: 0 288 954
Size: 288 666
BstXI CCAn_nnnn'nTGG
Cuts at: 0 633 954
Size: 633 321
BstYI r'GATC_y
Cuts at: 0 90 380 954
Size: 90 290 574
Fragments arranged by size:
574 290 90
BtgI C'CryG_G
Cuts at: 0 335 896 954
Size: 335 561 58
Fragments arranged by size:
561 335 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 315 954
Size: 315 639
EaeI y'GGCC_r
Cuts at: 0 2 45 276 304 338 608 823 885
Size: 2 43 231 28 34 270 215 62
Cuts at: 885 899 954
Size: 14 55
Fragments arranged by size:
270 231 215 62 55 43 34 28
14 2
EagI C'GGCC_G
Cuts at: 0 304 608 899 954
Size: 304 304 291 55
Fragments arranged by size:
304 304 291 55
EarI CTCTTCn'nnn_
Cuts at: 0 574 580 954
Size: 574 6 374
Fragments arranged by size:
574 374 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 439 901 954
Size: 439 462 53
Fragments arranged by size:
462 439 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 679 954
Size: 617 62 275
Fragments arranged by size:
617 275 62
EcoO109I rG'GnC_Cy
Cuts at: 0 25 111 112 166 792 954
Size: 25 86 1 54 626 162
Fragments arranged by size:
626 162 86 54 25 1
HaeII r_GCGC'y
Cuts at: 0 142 230 251 328 652 658 761 954
Size: 142 88 21 77 324 6 103 193
Fragments arranged by size:
324 193 142 103 88 77 21 6
KasI G'GCGC_C
Cuts at: 0 226 247 648 954
Size: 226 21 401 306
Fragments arranged by size:
401 306 226 21
MscI TGG'CCA
Cuts at: 0 4 340 887 954
Size: 4 336 547 67
Fragments arranged by size:
547 336 67 4
MslI CAynn'nnrTG
Cuts at: 0 334 954
Size: 334 620
MspA1I CmG'CkG
Cuts at: 0 357 898 954
Size: 357 541 56
Fragments arranged by size:
541 357 56
NaeI GCC'GGC
Cuts at: 0 906 954
Size: 906 48
NarI GG'CG_CC
Cuts at: 0 227 248 649 954
Size: 227 21 401 305
Fragments arranged by size:
401 305 227 21
NcoI C'CATG_G
Cuts at: 0 335 954
Size: 335 619
NgoMIV G'CCGG_C
Cuts at: 0 904 954
Size: 904 50
NotI GC'GGCC_GC
Cuts at: 0 304 899 954
Size: 304 595 55
Fragments arranged by size:
595 304 55
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 954
Size: 393 561
PpuMI rG'GwC_Cy
Cuts at: 0 166 954
Size: 166 788
PspOMI G'GGCC_C
Cuts at: 0 24 111 954
Size: 24 87 843
Fragments arranged by size:
843 87 24
PstI C_TGCA'G
Cuts at: 0 692 954
Size: 692 262
SacII CC_GC'GG
Cuts at: 0 899 954
Size: 899 55
SanDI GG'GwC_CC
Cuts at: 0 166 954
Size: 166 788
SbfI CC_TGCA'GG
Cuts at: 0 692 954
Size: 692 262
SfcI C'TryA_G
Cuts at: 0 688 930 954
Size: 688 242 24
Fragments arranged by size:
688 242 24
SfoI GGC'GCC
Cuts at: 0 228 249 650 954
Size: 228 21 401 304
Fragments arranged by size:
401 304 228 21
SmaI CCC'GGG
Cuts at: 0 74 171 233 789 954
Size: 74 97 62 556 165
Fragments arranged by size:
556 165 97 74 62
StuI AGG'CCT
Cuts at: 0 694 954
Size: 694 260
StyI C'CwwG_G
Cuts at: 0 335 774 954
Size: 335 439 180
Fragments arranged by size:
439 335 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 114 856 954
Size: 114 742 98
Fragments arranged by size:
742 114 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 831 863 954
Size: 831 32 91
Fragments arranged by size:
831 91 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 394 954
Size: 394 560
XmaI C'CCGG_G
Cuts at: 0 72 169 231 787 954
Size: 72 97 62 556 167
Fragments arranged by size:
556 167 97 72 62
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AvaI BanI BanII
BbeI BbvCI BglI BglII BlpI Bme1580I BpmI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseRI BsiEI Bsp1286I BspMI
BsrBI BsrFI BssHII BstAPI BstXI BstYI BtgI BtgZI
EaeI EagI EarI EciI Eco57MI EcoO109I HaeII KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI
PflMI PpuMI PspOMI PstI SacII SanDI SbfI SfcI
SfoI SmaI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI
BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BseYI
BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI NdeI NheI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SalI SapI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI