Restriction map for
pFN21AE3124
(Linear) (Six-base) MAPSORT of: KIEE3124.seq Check: 9410 from: 1 to: 939
KIEE3124.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 692 939
Size: 190 502 247
Fragments arranged by size:
502 247 190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 214 939
Size: 214 725
AlwNI CAG_nnn'CTG
Cuts at: 0 49 147 939
Size: 49 98 792
Fragments arranged by size:
792 98 49
ApaI G_GGCC'C
Cuts at: 0 927 939
Size: 927 12
AvaI C'yCGr_G
Cuts at: 0 295 939
Size: 295 644
BamHI G'GATC_C
Cuts at: 0 221 896 939
Size: 221 675 43
Fragments arranged by size:
675 221 43
BanI G'GyrC_C
Cuts at: 0 39 285 939
Size: 39 246 654
Fragments arranged by size:
654 246 39
BanII G_rGCy'C
Cuts at: 0 34 85 123 927 939
Size: 34 51 38 804 12
Fragments arranged by size:
804 51 38 34 12
BclI T'GATC_A
Cuts at: 0 425 939
Size: 425 514
BlpI GC'TnA_GC
Cuts at: 0 33 939
Size: 33 906
Bme1580I G_kGCm'C
Cuts at: 0 79 927 939
Size: 79 848 12
Fragments arranged by size:
848 79 12
BmtI G_CTAG'C
Cuts at: 0 328 939
Size: 328 611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 939
Size: 835 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 748 939
Size: 748 191
BsaAI yAC'GTr
Cuts at: 0 99 939
Size: 99 840
BsaBI GATnn'nnATC
Cuts at: 0 901 939
Size: 901 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 8 38 939
Size: 8 30 901
Fragments arranged by size:
901 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 331 766 880 939
Size: 331 435 114 59
Fragments arranged by size:
435 331 114 59
BseYI C'CCAG_C
Cuts at: 0 85 939
Size: 85 854
BsiHKAI G_wGCw'C
Cuts at: 0 34 203 939
Size: 34 169 736
Fragments arranged by size:
736 169 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 269 939
Size: 269 670
Bsp1286I G_dGCh'C
Cuts at: 0 34 79 85 123 203 927 939
Size: 34 45 6 38 80 724 12
Fragments arranged by size:
724 80 45 38 34 12 6
BsrBI CCG'CTC
Cuts at: 0 870 876 939
Size: 870 6 63
Fragments arranged by size:
870 63 6
BssSI C'ACGA_G
Cuts at: 0 78 939
Size: 78 861
BstYI r'GATC_y
Cuts at: 0 221 896 939
Size: 221 675 43
Fragments arranged by size:
675 221 43
EaeI y'GGCC_r
Cuts at: 0 789 939
Size: 789 150
EarI CTCTTCn'nnn_
Cuts at: 0 359 939
Size: 359 580
EcoICRI GAG'CTC
Cuts at: 0 32 939
Size: 32 907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 692 835 939
Size: 190 502 143 104
Fragments arranged by size:
502 190 143 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 856 939
Size: 856 83
EcoO109I rG'GnC_Cy
Cuts at: 0 417 597 796 939
Size: 417 180 199 143
Fragments arranged by size:
417 199 180 143
HaeII r_GCGC'y
Cuts at: 0 843 912 939
Size: 843 69 27
Fragments arranged by size:
843 69 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 939
Size: 559 32 348
Fragments arranged by size:
559 348 32
MslI CAynn'nnrTG
Cuts at: 0 196 939
Size: 196 743
MspA1I CmG'CkG
Cuts at: 0 37 46 185 236 939
Size: 37 9 139 51 703
Fragments arranged by size:
703 139 51 37 9
NheI G'CTAG_C
Cuts at: 0 324 939
Size: 324 615
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 852 939
Size: 357 495 87
Fragments arranged by size:
495 357 87
PfoI T'CCnGG_A
Cuts at: 0 812 939
Size: 812 127
PpuMI rG'GwC_Cy
Cuts at: 0 417 597 796 939
Size: 417 180 199 143
Fragments arranged by size:
417 199 180 143
PspOMI G'GGCC_C
Cuts at: 0 923 939
Size: 923 16
SacI G_AGCT'C
Cuts at: 0 34 939
Size: 34 905
ScaI AGT'ACT
Cuts at: 0 340 939
Size: 340 599
StuI AGG'CCT
Cuts at: 0 356 939
Size: 356 583
StyI C'CwwG_G
Cuts at: 0 351 437 719 939
Size: 351 86 282 220
Fragments arranged by size:
351 282 220 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 939
Size: 103 836
TatI w'GTAC_w
Cuts at: 0 338 939
Size: 338 601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8 40 939
Size: 8 32 899
Fragments arranged by size:
899 32 8
Enzymes that do cut:
AcuI AlfI AlwNI ApaI AvaI BamHI BanI BanII
BclI BlpI Bme1580I BmtI BpmI BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BsrBI BssSI
BstYI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I MslI MspA1I NheI PflMI PfoI PpuMI PspOMI
SacI ScaI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BmgBI BmrI BplI Bpu10I BpuEI
BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI