Restriction map for
pFN21AE3121
(Linear) (Six-base) MAPSORT of: KIEE3121.seq Check: 6653 from: 1 to: 921
KIEE3121.orf
With 182 enzymes: SgfI *
March 22, 2012 16:15 ..
AatII G_ACGT'C
Cuts at: 0 353 921
Size: 353 568
AflIII A'CryG_T
Cuts at: 0 644 921
Size: 644 277
AhdI GACnn_n'nnGTC
Cuts at: 0 64 921
Size: 64 857
AlwNI CAG_nnn'CTG
Cuts at: 0 817 921
Size: 817 104
ApoI r'AATT_y
Cuts at: 0 328 921
Size: 328 593
AvaI C'yCGr_G
Cuts at: 0 98 158 211 921
Size: 98 60 53 710
Fragments arranged by size:
710 98 60 53
BamHI G'GATC_C
Cuts at: 0 34 109 921
Size: 34 75 812
Fragments arranged by size:
812 75 34
BanI G'GyrC_C
Cuts at: 0 27 44 921
Size: 27 17 877
Fragments arranged by size:
877 27 17
BanII G_rGCy'C
Cuts at: 0 199 921
Size: 199 722
BbeI G_GCGC'C
Cuts at: 0 31 48 921
Size: 31 17 873
Fragments arranged by size:
873 31 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 319 921
Size: 319 602
BglI GCCn_nnn'nGGC
Cuts at: 0 160 921
Size: 160 761
BmgBI CAC'GTC
Cuts at: 0 645 921
Size: 645 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 921
Size: 881 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 921
Size: 651 270
BsaI GGTCTCn'nnnn_
Cuts at: 0 16 921
Size: 16 905
BsaHI Gr'CG_yC
Cuts at: 0 28 45 350 921
Size: 28 17 305 571
Fragments arranged by size:
571 305 28 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 841 871 921
Size: 358 30 453 30 50
Fragments arranged by size:
453 358 50 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 739 790 921
Size: 739 51 131
Fragments arranged by size:
739 131 51
BseYI C'CCAG_C
Cuts at: 0 149 437 921
Size: 149 288 484
Fragments arranged by size:
484 288 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 484 921
Size: 247 237 437
Fragments arranged by size:
437 247 237
BsiEI CG_ry'CG
Cuts at: 0 7 921
Size: 7 914
Bsp1286I G_dGCh'C
Cuts at: 0 199 921
Size: 199 722
BsrBI CCG'CTC
Cuts at: 0 115 921
Size: 115 806
BsrDI GCAATG_nn'
Cuts at: 0 912 921
Size: 912 9
BstYI r'GATC_y
Cuts at: 0 34 109 626 921
Size: 34 75 517 295
Fragments arranged by size:
517 295 75 34
Bsu36I CC'TnA_GG
Cuts at: 0 204 497 921
Size: 204 293 424
Fragments arranged by size:
424 293 204
BtgI C'CryG_G
Cuts at: 0 217 393 806 921
Size: 217 176 413 115
Fragments arranged by size:
413 217 176 115
EaeI y'GGCC_r
Cuts at: 0 136 396 921
Size: 136 260 525
Fragments arranged by size:
525 260 136
EarI CTCTTCn'nnn_
Cuts at: 0 703 716 921
Size: 703 13 205
Fragments arranged by size:
703 205 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 921
Size: 881 40
EcoO109I rG'GnC_Cy
Cuts at: 0 208 921
Size: 208 713
EcoRI G'AATT_C
Cuts at: 0 328 921
Size: 328 593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 814 921
Size: 814 107
HaeII r_GCGC'y
Cuts at: 0 31 48 166 234 921
Size: 31 17 118 68 687
Fragments arranged by size:
687 118 68 31 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 50 82 259 291 358 390 921
Size: 28 22 32 177 32 67 32 531
Fragments arranged by size:
531 177 67 32 32 32 28 22
HincII GTy'rAC
Cuts at: 0 362 921
Size: 362 559
HpaI GTT'AAC
Cuts at: 0 362 921
Size: 362 559
KasI G'GCGC_C
Cuts at: 0 27 44 921
Size: 27 17 877
Fragments arranged by size:
877 27 17
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 590 921
Size: 362 228 331
Fragments arranged by size:
362 331 228
MscI TGG'CCA
Cuts at: 0 398 921
Size: 398 523
MslI CAynn'nnrTG
Cuts at: 0 643 770 921
Size: 643 127 151
Fragments arranged by size:
643 151 127
MspA1I CmG'CkG
Cuts at: 0 141 219 741 921
Size: 141 78 522 180
Fragments arranged by size:
522 180 141 78
NarI GG'CG_CC
Cuts at: 0 28 45 921
Size: 28 17 876
Fragments arranged by size:
876 28 17
NcoI C'CATG_G
Cuts at: 0 806 921
Size: 806 115
NspI r_CATG'y
Cuts at: 0 323 921
Size: 323 598
PflMI CCAn_nnn'nTGG
Cuts at: 0 55 846 921
Size: 55 791 75
Fragments arranged by size:
791 75 55
PshAI GACnn'nnGTC
Cuts at: 0 349 921
Size: 349 572
SacII CC_GC'GG
Cuts at: 0 220 921
Size: 220 701
SfcI C'TryA_G
Cuts at: 0 451 921
Size: 451 470
SfoI GGC'GCC
Cuts at: 0 29 46 921
Size: 29 17 875
Fragments arranged by size:
875 29 17
SmaI CCC'GGG
Cuts at: 0 100 160 213 921
Size: 100 60 53 708
Fragments arranged by size:
708 100 60 53
SmlI C'TyrA_G
Cuts at: 0 630 921
Size: 630 291
SrfI GCCC'GGGC
Cuts at: 0 160 921
Size: 160 761
SspI AAT'ATT
Cuts at: 0 300 890 921
Size: 300 590 31
Fragments arranged by size:
590 300 31
StyI C'CwwG_G
Cuts at: 0 806 921
Size: 806 115
Tth111I GACn'n_nGTC
Cuts at: 0 8 921
Size: 8 913
XmaI C'CCGG_G
Cuts at: 0 98 158 211 921
Size: 98 60 53 710
Fragments arranged by size:
710 98 60 53
XmnI GAAnn'nnTTC
Cuts at: 0 758 921
Size: 758 163
ZraI GAC'GTC
Cuts at: 0 351 921
Size: 351 570
Enzymes that do cut:
AatII AflIII AhdI AlwNI ApoI AvaI BamHI BanI
BanII BbeI BbsI BglI BmgBI BpmI BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BsrBI
BsrDI BstYI Bsu36I BtgI EaeI EarI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII HpaI KasI MmeI
MscI MslI MspA1I NarI NcoI NspI PflMI PshAI
SacII SfcI SfoI SmaI SmlI SrfI SspI StyI
Tth111I XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI Bpu10I BsaAI BsaBI
BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI
HindIII KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
StuI SwaI TaqII TatI TstI XbaI XcmI XhoI