Restriction map for
pFN21AE3118
(Linear) (Six-base) MAPSORT of: KIEE3118.seq Check: 2283 from: 1 to: 876
KIEE3118.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 876
Size: 768 108
AflII C'TTAA_G
Cuts at: 0 447 876
Size: 447 429
ApoI r'AATT_y
Cuts at: 0 96 307 876
Size: 96 211 569
Fragments arranged by size:
569 211 96
AseI AT'TA_AT
Cuts at: 0 736 876
Size: 736 140
AvaI C'yCGr_G
Cuts at: 0 187 876
Size: 187 689
BanI G'GyrC_C
Cuts at: 0 6 72 79 876
Size: 6 66 7 797
Fragments arranged by size:
797 66 7 6
BanII G_rGCy'C
Cuts at: 0 212 442 590 876
Size: 212 230 148 286
Fragments arranged by size:
286 230 212 148
BbeI G_GCGC'C
Cuts at: 0 83 876
Size: 83 793
BglII A'GATC_T
Cuts at: 0 707 876
Size: 707 169
Bme1580I G_kGCm'C
Cuts at: 0 11 876
Size: 11 865
BmgBI CAC'GTC
Cuts at: 0 216 876
Size: 216 660
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 263 876
Size: 263 613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 456 876
Size: 285 171 420
Fragments arranged by size:
420 285 171
BsaI GGTCTCn'nnnn_
Cuts at: 0 34 518 876
Size: 34 484 358
Fragments arranged by size:
484 358 34
BsaHI Gr'CG_yC
Cuts at: 0 80 624 876
Size: 80 544 252
Fragments arranged by size:
544 252 80
BsaWI w'CCGG_w
Cuts at: 0 126 876
Size: 126 750
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 250 280 649 679 876
Size: 17 30 203 30 369 30 197
Fragments arranged by size:
369 203 197 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 307 876
Size: 307 569
BseYI C'CCAG_C
Cuts at: 0 636 876
Size: 636 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 876
Size: 724 152
BsiHKAI G_wGCw'C
Cuts at: 0 442 876
Size: 442 434
BsmBI CGTCTCn'nnnn_
Cuts at: 0 319 631 876
Size: 319 312 245
Fragments arranged by size:
319 312 245
Bsp1286I G_dGCh'C
Cuts at: 0 11 212 442 590 876
Size: 11 201 230 148 286
Fragments arranged by size:
286 230 201 148 11
BspEI T'CCGG_A
Cuts at: 0 126 876
Size: 126 750
BspHI T'CATG_A
Cuts at: 0 407 876
Size: 407 469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 295 876
Size: 295 581
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 724 876
Size: 18 706 152
Fragments arranged by size:
706 152 18
BstYI r'GATC_y
Cuts at: 0 234 333 707 876
Size: 234 99 374 169
Fragments arranged by size:
374 234 169 99
BtgI C'CryG_G
Cuts at: 0 11 528 876
Size: 11 517 348
Fragments arranged by size:
517 348 11
EarI CTCTTCn'nnn_
Cuts at: 0 47 787 876
Size: 47 740 89
Fragments arranged by size:
740 89 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 130 876
Size: 130 746
EcoICRI GAG'CTC
Cuts at: 0 440 876
Size: 440 436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 876
Size: 768 108
EcoO109I rG'GnC_Cy
Cuts at: 0 419 876
Size: 419 457
EcoRI G'AATT_C
Cuts at: 0 307 876
Size: 307 569
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 450 876
Size: 450 426
HaeII r_GCGC'y
Cuts at: 0 83 876
Size: 83 793
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 705 737 876
Size: 705 32 139
Fragments arranged by size:
705 139 32
KasI G'GCGC_C
Cuts at: 0 79 876
Size: 79 797
MspA1I CmG'CkG
Cuts at: 0 49 616 876
Size: 49 567 260
Fragments arranged by size:
567 260 49
NarI GG'CG_CC
Cuts at: 0 80 876
Size: 80 796
NcoI C'CATG_G
Cuts at: 0 528 876
Size: 528 348
NruI TCG'CGA
Cuts at: 0 63 876
Size: 63 813
NspI r_CATG'y
Cuts at: 0 492 690 876
Size: 492 198 186
Fragments arranged by size:
492 198 186
PpuMI rG'GwC_Cy
Cuts at: 0 419 876
Size: 419 457
PvuII CAG'CTG
Cuts at: 0 616 876
Size: 616 260
SacI G_AGCT'C
Cuts at: 0 442 876
Size: 442 434
SapI GCTCTTCn'nnn_
Cuts at: 0 787 876
Size: 787 89
SfoI GGC'GCC
Cuts at: 0 81 876
Size: 81 795
SmlI C'TyrA_G
Cuts at: 0 300 435 447 876
Size: 300 135 12 429
Fragments arranged by size:
429 300 135 12
StyI C'CwwG_G
Cuts at: 0 528 876
Size: 528 348
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 250 282 649 681 876
Size: 250 32 367 32 195
Fragments arranged by size:
367 250 195 32 32
Enzymes that do cut:
AcuI AflII ApoI AseI AvaI BanI BanII BbeI
BglII Bme1580I BmgBI BplI BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BstXI BstYI BtgI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I KasI MspA1I
NarI NcoI NruI NspI PpuMI PvuII SacI SapI
SfoI SmlI StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmrI BmtI BpmI Bpu10I
BsaAI BsaBI BsiEI BsiWI BsmI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EcoNI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NaeI
NdeI NgoMIV NheI NotI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI