(Linear) (Six-base) MAPSORT of: KIEE3116.seq  Check: 8148  from: 1  to: 873



KIEE3116.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     873 
   Size:        347     526
 
ApoI r'AATT_y

Cuts at:      0     143     154     584     634     873 
   Size:        143      11     430      50     239

  Fragments arranged by size: 

                 430     239     143      50      11
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      83     116     873 
   Size:         83      33     757

  Fragments arranged by size: 

                 757      83      33
 
BbvCI CC'TCA_GC

Cuts at:      0     478     873 
   Size:        478     395
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     843     873 
   Size:        843      30
 
BclI T'GATC_A

Cuts at:      0     639     873 
   Size:        639     234
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     873 
   Size:        151     722
 
Bpu10I CC'TnA_GC

Cuts at:      0     478     873 
   Size:        478     395
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     617     873 
   Size:        617     256
 
BsmI GAATG_Cn'

Cuts at:      0     544     873 
   Size:        544     329
 
BspHI T'CATG_A

Cuts at:      0     642     873 
   Size:        642     231
 
BsrGI T'GTAC_A

Cuts at:      0     687     873 
   Size:        687     186
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     316     873 
   Size:        316     557
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     306     873 
   Size:        306     567
 
BtgI C'CryG_G

Cuts at:      0      56     873 
   Size:         56     817
 
ClaI AT'CG_AT

Cuts at:      0      77     873 
   Size:         77     796
 
EaeI y'GGCC_r

Cuts at:      0      53     873 
   Size:         53     820
 
EarI CTCTTCn'nnn_

Cuts at:      0     110     494     873 
   Size:        110     384     379

  Fragments arranged by size: 

                 384     379     110
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     347     873 
   Size:        151     196     526

  Fragments arranged by size: 

                 526     196     151
 
EcoRV GAT'ATC

Cuts at:      0     240     873 
   Size:        240     633
 
MslI CAynn'nnrTG

Cuts at:      0     410     873 
   Size:        410     463
 
MspA1I CmG'CkG

Cuts at:      0     488     873 
   Size:        488     385
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     475     507     873 
   Size:        475      32     366

  Fragments arranged by size: 

                 475     366      32
 
PstI C_TGCA'G

Cuts at:      0     247     873 
   Size:        247     626
 
SfcI C'TryA_G

Cuts at:      0     243     873 
   Size:        243     630
 
SspI AAT'ATT

Cuts at:      0     595     873 
   Size:        595     278
 
TatI w'GTAC_w

Cuts at:      0     287     687     873 
   Size:        287     400     186

  Fragments arranged by size: 

                 400     287     186
 
XmnI GAAnn'nnTTC

Cuts at:      0      16     873 
   Size:         16     857
 

 Enzymes that do cut:

     AcuI     ApoI     BaeI    BbvCI    BciVI     BclI     BpmI   Bpu10I
     BsaI     BsmI    BspHI    BsrGI   BstAPI    BstXI     BtgI     ClaI
     EaeI     EarI  Eco57MI    EcoRV     MslI   MspA1I     PpiI     PstI
     SfcI     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI