Restriction map for
pFN21AE3115
(Linear) (Six-base) MAPSORT of: KIEE3115.seq Check: 2597 from: 1 to: 873
KIEE3115.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 671 873
Size: 227 444 202
Fragments arranged by size:
444 227 202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 131 873
Size: 131 742
ApoI r'AATT_y
Cuts at: 0 295 604 873
Size: 295 309 269
Fragments arranged by size:
309 295 269
AvaI C'yCGr_G
Cuts at: 0 6 64 695 873
Size: 6 58 631 178
Fragments arranged by size:
631 178 58 6
BanII G_rGCy'C
Cuts at: 0 7 669 805 873
Size: 7 662 136 68
Fragments arranged by size:
662 136 68 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 873
Size: 410 463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 873
Size: 516 357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 789 873
Size: 653 136 84
Fragments arranged by size:
653 136 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 810 840 873
Size: 810 30 33
Fragments arranged by size:
810 33 30
BseYI C'CCAG_C
Cuts at: 0 93 837 873
Size: 93 744 36
Fragments arranged by size:
744 93 36
BsiEI CG_ry'CG
Cuts at: 0 49 873
Size: 49 824
BsiHKAI G_wGCw'C
Cuts at: 0 7 873
Size: 7 866
BsmBI CGTCTCn'nnnn_
Cuts at: 0 751 873
Size: 751 122
Bsp1286I G_dGCh'C
Cuts at: 0 7 669 805 873
Size: 7 662 136 68
Fragments arranged by size:
662 136 68 7
BstXI CCAn_nnnn'nTGG
Cuts at: 0 837 873
Size: 837 36
BtsI GCAGTG_nn'
Cuts at: 0 644 873
Size: 644 229
DrdI GACnn_nn'nnGTC
Cuts at: 0 79 873
Size: 79 794
EarI CTCTTCn'nnn_
Cuts at: 0 179 499 584 873
Size: 179 320 85 289
Fragments arranged by size:
320 289 179 85
EcoICRI GAG'CTC
Cuts at: 0 5 873
Size: 5 868
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 516 671 873
Size: 227 289 155 202
Fragments arranged by size:
289 227 202 155
EcoO109I rG'GnC_Cy
Cuts at: 0 98 857 873
Size: 98 759 16
Fragments arranged by size:
759 98 16
EcoRI G'AATT_C
Cuts at: 0 604 873
Size: 604 269
HaeII r_GCGC'y
Cuts at: 0 14 32 94 873
Size: 14 18 62 779
Fragments arranged by size:
779 62 18 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 180 212 626 658 873
Size: 180 32 414 32 215
Fragments arranged by size:
414 215 180 32 32
MfeI C'AATT_G
Cuts at: 0 497 873
Size: 497 376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 598 873
Size: 29 569 275
Fragments arranged by size:
569 275 29
MslI CAynn'nnrTG
Cuts at: 0 113 725 873
Size: 113 612 148
Fragments arranged by size:
612 148 113
MspA1I CmG'CkG
Cuts at: 0 429 873
Size: 429 444
PpuMI rG'GwC_Cy
Cuts at: 0 98 873
Size: 98 775
PstI C_TGCA'G
Cuts at: 0 651 873
Size: 651 222
PvuII CAG'CTG
Cuts at: 0 429 873
Size: 429 444
SacI G_AGCT'C
Cuts at: 0 7 873
Size: 7 866
SanDI GG'GwC_CC
Cuts at: 0 98 873
Size: 98 775
SfcI C'TryA_G
Cuts at: 0 647 710 873
Size: 647 63 163
Fragments arranged by size:
647 163 63
SmaI CCC'GGG
Cuts at: 0 66 873
Size: 66 807
SmlI C'TyrA_G
Cuts at: 0 668 804 873
Size: 668 136 69
Fragments arranged by size:
668 136 69
SspI AAT'ATT
Cuts at: 0 312 873
Size: 312 561
StyI C'CwwG_G
Cuts at: 0 584 873
Size: 584 289
TatI w'GTAC_w
Cuts at: 0 622 873
Size: 622 251
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 810 842 873
Size: 810 32 31
Fragments arranged by size:
810 32 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 861 873
Size: 861 12
XmaI C'CCGG_G
Cuts at: 0 64 873
Size: 64 809
Enzymes that do cut:
AcuI AlfI ApoI AvaI BanII BbsI BpmI BpuEI
BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BstXI BtsI
DrdI EarI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I
MfeI MmeI MslI MspA1I PpuMI PstI PvuII SacI
SanDI SfcI SmaI SmlI SspI StyI TatI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BsgI BsiWI BsmI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII EaeI EagI EciI EcoNI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XbaI XhoI
XmnI ZraI