Restriction map for
pFN21AE3112
(Linear) (Six-base) MAPSORT of: KIEE3112.seq Check: 500 from: 1 to: 852
KIEE3112.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 342 852
Size: 342 510
ApoI r'AATT_y
Cuts at: 0 258 852
Size: 258 594
AvaI C'yCGr_G
Cuts at: 0 647 852
Size: 647 205
BanII G_rGCy'C
Cuts at: 0 708 852
Size: 708 144
BbsI GAAGACnn'nnnn_
Cuts at: 0 158 852
Size: 158 694
BciVI GTATCCnnnnn_n'
Cuts at: 0 636 852
Size: 636 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 588 830 852
Size: 588 242 22
Fragments arranged by size:
588 242 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 804 852
Size: 804 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 852
Size: 693 159
BsaAI yAC'GTr
Cuts at: 0 27 852
Size: 27 825
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 695 725 852
Size: 695 30 127
Fragments arranged by size:
695 127 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 122 796 852
Size: 122 674 56
Fragments arranged by size:
674 122 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 852
Size: 344 508
BsiHKAI G_wGCw'C
Cuts at: 0 44 708 852
Size: 44 664 144
Fragments arranged by size:
664 144 44
BsiWI C'GTAC_G
Cuts at: 0 23 852
Size: 23 829
BsmI GAATG_Cn'
Cuts at: 0 471 852
Size: 471 381
Bsp1286I G_dGCh'C
Cuts at: 0 44 708 852
Size: 44 664 144
Fragments arranged by size:
664 144 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 479 852
Size: 479 373
BsrDI GCAATG_nn'
Cuts at: 0 350 852
Size: 350 502
BstXI CCAn_nnnn'nTGG
Cuts at: 0 636 852
Size: 636 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 446 692 852
Size: 446 246 160
Fragments arranged by size:
446 246 160
BtsI GCAGTG_nn'
Cuts at: 0 601 852
Size: 601 251
DraI TTT'AAA
Cuts at: 0 768 852
Size: 768 84
EarI CTCTTCn'nnn_
Cuts at: 0 519 852
Size: 519 333
EcoICRI GAG'CTC
Cuts at: 0 706 852
Size: 706 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 852
Size: 693 159
EcoNI CCTnn'n_nnAGG
Cuts at: 0 267 852
Size: 267 585
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 779 852
Size: 779 73
HincII GTy'rAC
Cuts at: 0 686 852
Size: 686 166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 852
Size: 126 726
MslI CAynn'nnrTG
Cuts at: 0 634 852
Size: 634 218
MspA1I CmG'CkG
Cuts at: 0 328 852
Size: 328 524
NdeI CA'TA_TG
Cuts at: 0 454 852
Size: 454 398
NspI r_CATG'y
Cuts at: 0 537 852
Size: 537 315
PflMI CCAn_nnn'nTGG
Cuts at: 0 820 852
Size: 820 32
PsiI TTA'TAA
Cuts at: 0 662 852
Size: 662 190
PstI C_TGCA'G
Cuts at: 0 430 852
Size: 430 422
PvuII CAG'CTG
Cuts at: 0 328 852
Size: 328 524
SacI G_AGCT'C
Cuts at: 0 708 852
Size: 708 144
SfcI C'TryA_G
Cuts at: 0 426 852
Size: 426 426
SmlI C'TyrA_G
Cuts at: 0 647 852
Size: 647 205
SphI G_CATG'C
Cuts at: 0 537 852
Size: 537 315
SspI AAT'ATT
Cuts at: 0 719 852
Size: 719 133
TatI w'GTAC_w
Cuts at: 0 527 852
Size: 527 325
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 244 276 852
Size: 244 32 576
Fragments arranged by size:
576 244 32
XhoI C'TCGA_G
Cuts at: 0 647 852
Size: 647 205
Enzymes that do cut:
AlwNI ApoI AvaI BanII BbsI BciVI BmrI BplI
BpmI BsaAI BsaXI BseRI BsgI BsiHKAI BsiWI BsmI
Bsp1286I BspMI BsrDI BstXI BtgZI BtsI DraI EarI
EcoICRI Eco57MI EcoNI FalI HincII MmeI MslI MspA1I
NdeI NspI PflMI PsiI PstI PvuII SacI SfcI
SmlI SphI SspI TatI TstI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BseYI BsiEI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI StyI SwaI TaqII
Tth111I XbaI XcmI XmaI XmnI ZraI