Restriction map for
pFN21AE3102
(Linear) (Six-base) MAPSORT of: KIEE3102.seq Check: 6816 from: 1 to: 807
KIEE3102.orf
With 182 enzymes: SgfI *
March 22, 2012 16:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 807
Size: 695 112
AflII C'TTAA_G
Cuts at: 0 322 807
Size: 322 485
BanII G_rGCy'C
Cuts at: 0 42 807
Size: 42 765
BciVI GTATCCnnnnn_n'
Cuts at: 0 604 807
Size: 604 203
BfrBI ATG'CAT
Cuts at: 0 3 807
Size: 3 804
BglI GCCn_nnn'nGGC
Cuts at: 0 232 807
Size: 232 575
BmrI ACTGGGnnnn_n'
Cuts at: 0 497 807
Size: 497 310
BsaI GGTCTCn'nnnn_
Cuts at: 0 23 183 807
Size: 23 160 624
Fragments arranged by size:
624 160 23
BsaWI w'CCGG_w
Cuts at: 0 219 807
Size: 219 588
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 807
Size: 328 479
BsiEI CG_ry'CG
Cuts at: 0 194 807
Size: 194 613
BsmBI CGTCTCn'nnnn_
Cuts at: 0 59 267 807
Size: 59 208 540
Fragments arranged by size:
540 208 59
Bsp1286I G_dGCh'C
Cuts at: 0 42 807
Size: 42 765
BspEI T'CCGG_A
Cuts at: 0 219 807
Size: 219 588
BsrBI CCG'CTC
Cuts at: 0 135 807
Size: 135 672
BsrDI GCAATG_nn'
Cuts at: 0 477 807
Size: 477 330
BsrGI T'GTAC_A
Cuts at: 0 403 807
Size: 403 404
BstYI r'GATC_y
Cuts at: 0 363 807
Size: 363 444
BtgI C'CryG_G
Cuts at: 0 34 807
Size: 34 773
ClaI AT'CG_AT
Cuts at: 0 597 807
Size: 597 210
DraI TTT'AAA
Cuts at: 0 11 807
Size: 11 796
EaeI y'GGCC_r
Cuts at: 0 191 199 233 807
Size: 191 8 34 574
Fragments arranged by size:
574 191 34 8
EagI C'GGCC_G
Cuts at: 0 191 807
Size: 191 616
EarI CTCTTCn'nnn_
Cuts at: 0 57 807
Size: 57 750
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 158 164 291 807
Size: 158 6 127 516
Fragments arranged by size:
516 158 127 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 807
Size: 695 112
EcoNI CCTnn'n_nnAGG
Cuts at: 0 89 807
Size: 89 718
EcoO109I rG'GnC_Cy
Cuts at: 0 94 277 333 807
Size: 94 183 56 474
Fragments arranged by size:
474 183 94 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 807
Size: 157 32 618
Fragments arranged by size:
618 157 32
HincII GTy'rAC
Cuts at: 0 399 807
Size: 399 408
HpaI GTT'AAC
Cuts at: 0 399 807
Size: 399 408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 807
Size: 684 123
NotI GC'GGCC_GC
Cuts at: 0 191 807
Size: 191 616
NsiI A_TGCA'T
Cuts at: 0 5 807
Size: 5 802
PasI CC'CwG_GG
Cuts at: 0 281 807
Size: 281 526
PpuMI rG'GwC_Cy
Cuts at: 0 94 277 333 807
Size: 94 183 56 474
Fragments arranged by size:
474 183 94 56
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 738 770 807
Size: 738 32 37
Fragments arranged by size:
738 37 32
SfcI C'TryA_G
Cuts at: 0 272 644 807
Size: 272 372 163
Fragments arranged by size:
372 272 163
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 232 807
Size: 232 575
SmlI C'TyrA_G
Cuts at: 0 322 807
Size: 322 485
StyI C'CwwG_G
Cuts at: 0 606 807
Size: 606 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 456 807
Size: 165 291 351
Fragments arranged by size:
351 291 165
TatI w'GTAC_w
Cuts at: 0 403 807
Size: 403 404
Enzymes that do cut:
AcuI AflII BanII BciVI BfrBI BglI BmrI BsaI
BsaWI BseRI BsiEI BsmBI Bsp1286I BspEI BsrBI BsrDI
BsrGI BstYI BtgI ClaI DraI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I Hin4I HincII HpaI MmeI
NotI NsiI PasI PpuMI PsrI SfcI SfiI SmlI
StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BclI BglII BlpI Bme1580I
BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaXI BseYI BsgI BsiHKAI BsiWI BsmI BspHI
BspMI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI CspCI DraIII DrdI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NruI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI