Restriction map for
pFN21AE3101
(Linear) (Six-base) MAPSORT of: KIEE3101.seq Check: 8204 from: 1 to: 801
KIEE3101.orf
With 182 enzymes: SgfI *
March 22, 2012 16:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 801
Size: 483 318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 508 801
Size: 508 293
AlwNI CAG_nnn'CTG
Cuts at: 0 24 257 801
Size: 24 233 544
Fragments arranged by size:
544 233 24
ApoI r'AATT_y
Cuts at: 0 394 801
Size: 394 407
BanI G'GyrC_C
Cuts at: 0 56 193 401 801
Size: 56 137 208 400
Fragments arranged by size:
400 208 137 56
BanII G_rGCy'C
Cuts at: 0 311 801
Size: 311 490
BbsI GAAGACnn'nnnn_
Cuts at: 0 736 801
Size: 736 65
BbvCI CC'TCA_GC
Cuts at: 0 149 801
Size: 149 652
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 636 670 801
Size: 636 34 131
Fragments arranged by size:
636 131 34
BclI T'GATC_A
Cuts at: 0 686 801
Size: 686 115
BfrBI ATG'CAT
Cuts at: 0 3 282 801
Size: 3 279 519
Fragments arranged by size:
519 279 3
BglII A'GATC_T
Cuts at: 0 162 801
Size: 162 639
Bme1580I G_kGCm'C
Cuts at: 0 497 801
Size: 497 304
BmrI ACTGGGnnnn_n'
Cuts at: 0 321 801
Size: 321 480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 801
Size: 608 193
Bpu10I CC'TnA_GC
Cuts at: 0 149 801
Size: 149 652
BsaI GGTCTCn'nnnn_
Cuts at: 0 472 801
Size: 472 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 703 733 801
Size: 703 30 68
Fragments arranged by size:
703 68 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 166 362 724 801
Size: 166 196 362 77
Fragments arranged by size:
362 196 166 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 801
Size: 651 150
Bsp1286I G_dGCh'C
Cuts at: 0 311 497 801
Size: 311 186 304
Fragments arranged by size:
311 304 186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 242 490 801
Size: 242 248 311
Fragments arranged by size:
311 248 242
BstYI r'GATC_y
Cuts at: 0 162 801
Size: 162 639
BtsI GCAGTG_nn'
Cuts at: 0 182 801
Size: 182 619
EaeI y'GGCC_r
Cuts at: 0 66 801
Size: 66 735
EarI CTCTTCn'nnn_
Cuts at: 0 380 801
Size: 380 421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 608 801
Size: 483 125 193
Fragments arranged by size:
483 193 125
EcoO109I rG'GnC_Cy
Cuts at: 0 323 801
Size: 323 478
MslI CAynn'nnrTG
Cuts at: 0 62 646 743 801
Size: 62 584 97 58
Fragments arranged by size:
584 97 62 58
MspA1I CmG'CkG
Cuts at: 0 21 801
Size: 21 780
NsiI A_TGCA'T
Cuts at: 0 5 284 801
Size: 5 279 517
Fragments arranged by size:
517 279 5
NspI r_CATG'y
Cuts at: 0 763 801
Size: 763 38
PfoI T'CCnGG_A
Cuts at: 0 585 801
Size: 585 216
PpuMI rG'GwC_Cy
Cuts at: 0 323 801
Size: 323 478
PstI C_TGCA'G
Cuts at: 0 207 487 801
Size: 207 280 314
Fragments arranged by size:
314 280 207
PvuII CAG'CTG
Cuts at: 0 21 801
Size: 21 780
SanDI GG'GwC_CC
Cuts at: 0 323 801
Size: 323 478
SbfI CC_TGCA'GG
Cuts at: 0 207 487 801
Size: 207 280 314
Fragments arranged by size:
314 280 207
SfcI C'TryA_G
Cuts at: 0 203 483 801
Size: 203 280 318
Fragments arranged by size:
318 280 203
StyI C'CwwG_G
Cuts at: 0 106 801
Size: 106 695
TatI w'GTAC_w
Cuts at: 0 468 801
Size: 468 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 703 735 801
Size: 703 32 66
Fragments arranged by size:
703 66 32
Tth111I GACn'n_nGTC
Cuts at: 0 327 801
Size: 327 474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 346 801
Size: 346 455
Enzymes that do cut:
AcuI AlfI AlwNI ApoI BanI BanII BbsI BbvCI
BcgI BclI BfrBI BglII Bme1580I BmrI BpmI Bpu10I
BsaI BsaXI BseRI BsgI Bsp1286I BspMI BstYI BtsI
EaeI EarI Eco57MI EcoO109I MslI MspA1I NsiI NspI
PfoI PpuMI PstI PvuII SanDI SbfI SfcI StyI
TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BciVI BglI
BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII XbaI XhoI XmaI XmnI ZraI