Restriction map for
pFN21AE3100
(Linear) (Six-base) MAPSORT of: KIEE3100.seq Check: 3186 from: 1 to: 6105
KIEE3100.orf
With 182 enzymes: SgfI *
March 22, 2012 16:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1311 2439 4350 6105
Size: 1311 1128 1911 1755
Fragments arranged by size:
1911 1755 1311 1128
AatII G_ACGT'C
Cuts at: 0 842 6105
Size: 842 5263
AccI GT'mk_AC
Cuts at: 0 620 1882 4745 6105
Size: 620 1262 2863 1360
Fragments arranged by size:
2863 1360 1262 620
Acc65I G'GTAC_C
Cuts at: 0 503 1070 1717 6105
Size: 503 567 647 4388
Fragments arranged by size:
4388 647 567 503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1849 2066 2185 2399 5572 5613 6105
Size: 1849 217 119 214 3173 41 492
Fragments arranged by size:
3173 1849 492 217 214 119 41
AflII C'TTAA_G
Cuts at: 0 2503 6105
Size: 2503 3602
AflIII A'CryG_T
Cuts at: 0 179 803 1877 6105
Size: 179 624 1074 4228
Fragments arranged by size:
4228 1074 624 179
AgeI A'CCGG_T
Cuts at: 0 1468 4473 6105
Size: 1468 3005 1632
Fragments arranged by size:
3005 1632 1468
AhdI GACnn_n'nnGTC
Cuts at: 0 2363 6105
Size: 2363 3742
AleI CACnn'nnGTG
Cuts at: 0 1726 1957 1987 2353 2404 3031 3133 3768
Size: 1726 231 30 366 51 627 102 635
Cuts at: 3768 3805 3834 4708 5281 5986 6105
Size: 37 29 874 573 705 119
Fragments arranged by size:
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119 102 51 37 30 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3786 3852 4070 4234 6105
Size: 3786 66 218 164 1871
Fragments arranged by size:
3786 1871 218 164 66
AlwNI CAG_nnn'CTG
Cuts at: 0 544 1317 1774 1918 2422 3811 4539 4557
Size: 544 773 457 144 504 1389 728 18
Cuts at: 4557 4896 5070 5097 5565 6105
Size: 339 174 27 468 540
Fragments arranged by size:
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339 174 144 27 18
ApaI G_GGCC'C
Cuts at: 0 395 2144 2514 3555 4486 5627 5679 5864
Size: 395 1749 370 1041 931 1141 52 185
Cuts at: 5864 6105
Size: 241
Fragments arranged by size:
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52
ApaLI G'TGCA_C
Cuts at: 0 1903 6105
Size: 1903 4202
AvaI C'yCGr_G
Cuts at: 0 412 596 962 1103 2514 3601 4033 4479
Size: 412 184 366 141 1411 1087 432 446
Cuts at: 4479 4825 6105
Size: 346 1280
Fragments arranged by size:
1411 1280 1087 446 432 412 366 346
184 141
AvrII C'CTAG_G
Cuts at: 0 2646 6105
Size: 2646 3459
BanI G'GyrC_C
Cuts at: 0 84 503 750 819 1070 1398 1689 1717
Size: 84 419 247 69 251 328 291 28
Cuts at: 1717 2344 2527 2626 2662 2740 2892 3070 3256
Size: 627 183 99 36 78 152 178 186
Cuts at: 3256 3343 3382 3424 3692 3762 3898 3982 4096
Size: 87 39 42 268 70 136 84 114
Cuts at: 4096 4120 4185 4210 4282 4792 4942 5003 5458
Size: 24 65 25 72 510 150 61 455
Cuts at: 5458 5527 6025 6105
Size: 69 498 80
Fragments arranged by size:
627 510 498 455 419 328 291 268
251 247 186 183 178 152 150 136
114 99 87 84 84 80 78 72
70 69 69 65 61 42 39 36
28 25 24
BanII G_rGCy'C
Cuts at: 0 131 395 1282 1999 2144 2514 2831 3484
Size: 131 264 887 717 145 370 317 653
Cuts at: 3484 3555 3602 3755 4486 4628 4820 4955 5627
Size: 71 47 153 731 142 192 135 672
Cuts at: 5627 5679 5794 5813 5821 5864 6105
Size: 52 115 19 8 43 241
Fragments arranged by size:
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241 192 153 145 142 135 131 115
71 52 47 43 19 8
BbeI G_GCGC'C
Cuts at: 0 754 2348 3386 3696 6105
Size: 754 1594 1038 310 2409
Fragments arranged by size:
2409 1594 1038 754 310
BbsI GAAGACnn'nnnn_
Cuts at: 0 1738 1837 6105
Size: 1738 99 4268
Fragments arranged by size:
4268 1738 99
BbvCI CC'TCA_GC
Cuts at: 0 2178 6105
Size: 2178 3927
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5742 5776 6105
Size: 5742 34 329
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 897 927 6105
Size: 897 30 5178
Fragments arranged by size:
5178 897 30
BclI T'GATC_A
Cuts at: 0 2768 2885 6105
Size: 2768 117 3220
Fragments arranged by size:
3220 2768 117
BglI GCCn_nnn'nGGC
Cuts at: 0 1522 1645 1654 2227 3409 3460 3550 3826
Size: 1522 123 9 573 1182 51 90 276
Cuts at: 3826 4548 4906 5836 6105
Size: 722 358 930 269
Fragments arranged by size:
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123 90 51 9
BlpI GC'TnA_GC
Cuts at: 0 3658 6105
Size: 3658 2447
Bme1580I G_kGCm'C
Cuts at: 0 395 419 1424 1596 1907 2144 2514 2549
Size: 395 24 1005 172 311 237 370 35
Cuts at: 2549 2743 3259 3346 3555 3841 3985 4024 4125
Size: 194 516 87 209 286 144 39 101
Cuts at: 4125 4285 4486 4945 5006 5461 5530 5627 5679
Size: 160 201 459 61 455 69 97 52
Cuts at: 5679 5864 6105
Size: 185 241
Fragments arranged by size:
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241 237 209 201 194 185 172 160
144 101 97 87 69 61 52 39
35 24
BmgBI CAC'GTC
Cuts at: 0 722 1880 5654 6105
Size: 722 1158 3774 451
Fragments arranged by size:
3774 1158 722 451
BmrI ACTGGGnnnn_n'
Cuts at: 0 728 2267 3467 3486 6105
Size: 728 1539 1200 19 2619
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5282 5754 6105
Size: 5282 472 351
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 566 961 1159 1168 1174 2444 3487
Size: 322 244 395 198 9 6 1270 1043
Cuts at: 3487 3766 3844 4389 4495 4900 5874 6044 6105
Size: 279 78 545 106 405 974 170 61
Fragments arranged by size:
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244 198 170 106 78 61 9 6
Bpu10I CC'TnA_GC
Cuts at: 0 2178 4622 6105
Size: 2178 2444 1483
Fragments arranged by size:
2444 2178 1483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1424 2742 5797 6105
Size: 1424 1318 3055 308
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 913 3049 3235 3322 3877 3961 4261 6105
Size: 913 2136 186 87 555 84 300 1844
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 180 804 6105
Size: 180 624 5301
Fragments arranged by size:
5301 624 180
BsaBI GATnn'nnATC
Cuts at: 0 3442 6105
Size: 3442 2663
BsaHI Gr'CG_yC
Cuts at: 0 379 751 839 2101 2345 3383 3693 4136
Size: 379 372 88 1262 244 1038 310 443
Cuts at: 4136 6105
Size: 1969
Fragments arranged by size:
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88
BsaWI w'CCGG_w
Cuts at: 0 81 1245 1468 3440 4473 5706 6105
Size: 81 1164 223 1972 1033 1233 399
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 856 886 1002 1032 1117 1147
Size: 409 30 417 30 116 30 85 30
Cuts at: 1147 1476 1506 6105
Size: 329 30 4599
Fragments arranged by size:
4599 417 409 329 116 85 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1779 4843 6105
Size: 1779 3064 1262
Fragments arranged by size:
3064 1779 1262
BseYI C'CCAG_C
Cuts at: 0 213 684 815 1098 1148 1287 1525 1710
Size: 213 471 131 283 50 139 238 185
Cuts at: 1710 1767 1931 2864 2912 3122 3745 3796 4189
Size: 57 164 933 48 210 623 51 393
Cuts at: 4189 4998 5114 5175 5221 5291 5313 5401 5800
Size: 809 116 61 46 70 22 88 399
Cuts at: 5800 5831 5863 6105
Size: 31 32 242
Fragments arranged by size:
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238 213 210 185 164 139 131 116
88 70 61 57 51 50 48 46
32 31 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 266 1330 3031 3157 3437 3646 5104
Size: 160 106 1064 1701 126 280 209 1458
Cuts at: 5104 5902 6105
Size: 798 203
Fragments arranged by size:
1701 1458 1064 798 280 209 203 160
126 106
BsiEI CG_ry'CG
Cuts at: 0 11 1658 2798 3566 4634 5015 6105
Size: 11 1647 1140 768 1068 381 1090
Fragments arranged by size:
1647 1140 1090 1068 768 381 11
BsiHKAI G_wGCw'C
Cuts at: 0 131 963 1907 2025 2430 3040 3562 3952
Size: 131 832 944 118 405 610 522 390
Cuts at: 3952 4252 4375 4628 4916 5718 5794 5813 5821
Size: 300 123 253 288 802 76 19 8
Cuts at: 5821 6105
Size: 284
Fragments arranged by size:
944 832 802 610 522 405 390 300
288 284 253 131 123 118 76 19
8
BsmI GAATG_Cn'
Cuts at: 0 3930 6105
Size: 3930 2175
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2271 2589 2673 3244 3331 3970 4270 6105
Size: 2271 318 84 571 87 639 300 1835
Fragments arranged by size:
2271 1835 639 571 318 300 87 84
Bsp1286I G_dGCh'C
Cuts at: 0 131 395 419 963 1282 1424 1596 1907
Size: 131 264 24 544 319 142 172 311
Cuts at: 1907 1999 2025 2144 2430 2514 2549 2743 2831
Size: 92 26 119 286 84 35 194 88
Cuts at: 2831 3040 3259 3346 3484 3555 3562 3602 3755
Size: 209 219 87 138 71 7 40 153
Cuts at: 3755 3841 3952 3985 4024 4125 4252 4285 4375
Size: 86 111 33 39 101 127 33 90
Cuts at: 4375 4486 4628 4820 4916 4945 4955 5006 5461
Size: 111 142 192 96 29 10 51 455
Cuts at: 5461 5530 5627 5679 5718 5794 5813 5821 5864
Size: 69 97 52 39 76 19 8 43
Cuts at: 5864 6105
Size: 241
Fragments arranged by size:
544 455 319 311 286 264 241 219
209 194 192 172 153 142 142 138
131 127 119 111 111 101 97 96
92 90 88 87 86 84 76 71
69 52 51 43 40 39 39 35
33 33 29 26 24 19 10 8
7
BspEI T'CCGG_A
Cuts at: 0 3440 5706 6105
Size: 3440 2266 399
Fragments arranged by size:
3440 2266 399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1311 1521 2439 4350 5396 6105
Size: 1311 210 918 1911 1046 709
Fragments arranged by size:
1911 1311 1046 918 709 210
BsrDI GCAATG_nn'
Cuts at: 0 327 2744 5964 6105
Size: 327 2417 3220 141
Fragments arranged by size:
3220 2417 327 141
BsrFI r'CCGG_y
Cuts at: 0 347 1468 3565 4473 4684 4945 5209 5295
Size: 347 1121 2097 908 211 261 264 86
Cuts at: 5295 6105
Size: 810
Fragments arranged by size:
2097 1121 908 810 347 264 261 211
86
BsrGI T'GTAC_A
Cuts at: 0 182 1001 6105
Size: 182 819 5104
Fragments arranged by size:
5104 819 182
BssSI C'ACGA_G
Cuts at: 0 856 3061 3247 3334 3889 3973 4273 6105
Size: 856 2205 186 87 555 84 300 1832
Fragments arranged by size:
2205 1832 856 555 300 186 87 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 443 4557 5097 5565 6105
Size: 443 4114 540 468 540
Fragments arranged by size:
4114 540 540 468 443
BstBI TT'CG_AA
Cuts at: 0 3226 6105
Size: 3226 2879
BstEII G'GTnAC_C
Cuts at: 0 1500 1947 1968 2172 6105
Size: 1500 447 21 204 3933
Fragments arranged by size:
3933 1500 447 204 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 885 930 1137 1959 1986 2250 3067 3202
Size: 885 45 207 822 27 264 817 135
Cuts at: 3202 4710 4806 5229 5469 6105
Size: 1508 96 423 240 636
Fragments arranged by size:
1508 885 822 817 636 423 264 240
207 135 96 45 27
BstYI r'GATC_y
Cuts at: 0 1673 4641 6105
Size: 1673 2968 1464
Fragments arranged by size:
2968 1673 1464
Bsu36I CC'TnA_GG
Cuts at: 0 1475 6105
Size: 1475 4630
BtgI C'CryG_G
Cuts at: 0 88 99 113 614 908 1211 1694 1736
Size: 88 11 14 501 294 303 483 42
Cuts at: 1736 2105 2348 2472 2549 4157 4546 4637 4877
Size: 369 243 124 77 1608 389 91 240
Cuts at: 4877 4955 6105
Size: 78 1150
Fragments arranged by size:
1608 1150 501 483 389 369 303 294
243 240 124 91 88 78 77 42
14 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2970 5931 6105
Size: 2970 2961 174
Fragments arranged by size:
2970 2961 174
BtsI GCAGTG_nn'
Cuts at: 0 972 1163 2025 2444 2777 3353 3829 4844
Size: 972 191 862 419 333 576 476 1015
Cuts at: 4844 6105
Size: 1261
Fragments arranged by size:
1261 1015 972 862 576 476 419 333
191
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2598 2633 2661 2696 3078 3113 5595 5630
Size: 2598 35 28 35 382 35 2482 35
Cuts at: 5630 5974 6009 6105
Size: 344 35 96
Fragments arranged by size:
2598 2482 382 344 96 35 35 35
35 35 28
DraIII CAC_nnn'GTG
Cuts at: 0 1591 2899 3052 3880 6105
Size: 1591 1308 153 828 2225
Fragments arranged by size:
2225 1591 1308 828 153
DrdI GACnn_nn'nnGTC
Cuts at: 0 4161 4425 6105
Size: 4161 264 1680
Fragments arranged by size:
4161 1680 264
EaeI y'GGCC_r
Cuts at: 0 8 102 116 464 535 848 969 1637
Size: 8 94 14 348 71 313 121 668
Cuts at: 1637 1646 1655 2324 2469 2717 2795 3083 3293
Size: 9 9 669 145 248 78 288 210
Cuts at: 3293 3452 3542 3563 3845 3995 4295 4631 4700
Size: 159 90 21 282 150 300 336 69
Cuts at: 4700 4775 4898 5012 5207 5249 5306 5351 5616
Size: 75 123 114 195 42 57 45 265
Cuts at: 5616 5780 5978 6105
Size: 164 198 127
Fragments arranged by size:
669 668 348 336 313 300 288 282
265 248 210 198 195 164 159 150
145 127 123 121 114 94 90 78
75 71 69 57 45 42 21 14
9 9 8
EagI C'GGCC_G
Cuts at: 0 8 1655 2795 3563 4631 5012 6105
Size: 8 1647 1140 768 1068 381 1093
Fragments arranged by size:
1647 1140 1093 1068 768 381 8
EarI CTCTTCn'nnn_
Cuts at: 0 355 761 1987 6105
Size: 355 406 1226 4118
Fragments arranged by size:
4118 1226 406 355
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1857 2577 4581 5008 6105
Size: 1857 720 2004 427 1097
Fragments arranged by size:
2004 1857 1097 720 427
EcoICRI GAG'CTC
Cuts at: 0 129 4626 5792 5811 5819 6105
Size: 129 4497 1166 19 8 286
Fragments arranged by size:
4497 1166 286 129 19 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 566 961 1159 1168 1174 1849 2066
Size: 322 244 395 198 9 6 675 217
Cuts at: 2066 2185 2399 2444 3487 3766 3844 4389 4495
Size: 119 214 45 1043 279 78 545 106
Cuts at: 4495 4900 5572 5613 5874 6044 6105
Size: 405 672 41 261 170 61
Fragments arranged by size:
1043 675 672 545 405 395 322 279
261 244 217 214 198 170 119 106
78 61 45 41 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1345 6105
Size: 1345 4760
EcoO109I rG'GnC_Cy
Cuts at: 0 392 480 582 690 1062 2110 2140 2141
Size: 392 88 102 108 372 1048 30 1
Cuts at: 2141 2510 2511 3551 3552 3678 4483 4525 4599
Size: 369 1 1040 1 126 805 42 74
Cuts at: 4599 4893 5145 5623 5624 5675 5676 6105
Size: 294 252 478 1 51 1 429
Fragments arranged by size:
1048 1040 805 478 429 392 372 369
294 252 126 108 102 88 74 51
42 30 1 1 1 1 1
FseI GG_CCGG'CC
Cuts at: 0 3569 6105
Size: 3569 2536
FspI TGC'GCA
Cuts at: 0 978 6105
Size: 978 5127
HaeII r_GCGC'y
Cuts at: 0 754 2348 3386 3466 3696 3938 5236 6105
Size: 754 1594 1038 80 230 242 1298 869
Fragments arranged by size:
1594 1298 1038 869 754 242 230 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1458 1490 2019 2051 2349 2381 4588 4620
Size: 1458 32 529 32 298 32 2207 32
Cuts at: 4620 4738 4770 6105
Size: 118 32 1335
Fragments arranged by size:
2207 1458 1335 529 298 118 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 750 2344 3382 3692 6105
Size: 750 1594 1038 310 2413
Fragments arranged by size:
2413 1594 1038 750 310
KpnI G_GTAC'C
Cuts at: 0 507 1074 1721 6105
Size: 507 567 647 4384
Fragments arranged by size:
4384 647 567 507
MfeI C'AATT_G
Cuts at: 0 5190 6105
Size: 5190 915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3065 3311 3893 3977 4277 4337 5297 5924
Size: 3065 246 582 84 300 60 960 627
Cuts at: 5924 6105
Size: 181
Fragments arranged by size:
3065 960 627 582 300 246 181 84
60
MscI TGG'CCA
Cuts at: 0 118 466 850 971 2471 2719 3454 3544
Size: 118 348 384 121 1500 248 735 90
Cuts at: 3544 4702 4900 5308 5353 5782 6105
Size: 1158 198 408 45 429 323
Fragments arranged by size:
1500 1158 735 429 408 384 348 323
248 198 121 118 90 45
MslI CAynn'nnrTG
Cuts at: 0 136 928 1417 1726 1741 1822 1900 1957
Size: 136 792 489 309 15 81 78 57
Cuts at: 1957 1987 2317 2353 2404 2788 2848 3031 3133
Size: 30 330 36 51 384 60 183 102
Cuts at: 3133 3529 3535 3701 3768 3805 3834 4102 4708
Size: 396 6 166 67 37 29 268 606
Cuts at: 4708 4804 5281 5848 5986 6105
Size: 96 477 567 138 119
Fragments arranged by size:
792 606 567 489 477 396 384 330
309 268 183 166 138 136 119 102
96 81 78 67 60 57 51 37
36 30 29 15 6
MspA1I CmG'CkG
Cuts at: 0 34 53 743 1317 1651 1660 1714 2152
Size: 34 19 690 574 334 9 54 438
Cuts at: 2152 2818 3166 3421 3718 4539 4861 5097 5550
Size: 666 348 255 297 821 322 236 453
Cuts at: 5550 5835 6105
Size: 285 270
Fragments arranged by size:
821 690 666 574 453 438 348 334
322 297 285 270 255 236 54 34
19 9
NaeI GCC'GGC
Cuts at: 0 3567 4686 5211 6105
Size: 3567 1119 525 894
Fragments arranged by size:
3567 1119 894 525
NarI GG'CG_CC
Cuts at: 0 751 2345 3383 3693 6105
Size: 751 1594 1038 310 2412
Fragments arranged by size:
2412 1594 1038 751 310
NcoI C'CATG_G
Cuts at: 0 908 1736 2549 4955 6105
Size: 908 828 813 2406 1150
Fragments arranged by size:
2406 1150 908 828 813
NgoMIV G'CCGG_C
Cuts at: 0 3565 4684 5209 6105
Size: 3565 1119 525 896
Fragments arranged by size:
3565 1119 896 525
NotI GC'GGCC_GC
Cuts at: 0 1655 6105
Size: 1655 4450
NspI r_CATG'y
Cuts at: 0 1827 6105
Size: 1827 4278
PasI CC'CwG_GG
Cuts at: 0 238 1596 3477 4377 5370 5671 6105
Size: 238 1358 1881 900 993 301 434
Fragments arranged by size:
1881 1358 993 900 434 301 238
PflMI CCAn_nnn'nTGG
Cuts at: 0 203 544 914 3577 4896 6105
Size: 203 341 370 2663 1319 1209
Fragments arranged by size:
2663 1319 1209 370 341 203
PfoI T'CCnGG_A
Cuts at: 0 524 6105
Size: 524 5581
PmlI CAC'GTG
Cuts at: 0 180 6105
Size: 180 5925
PpuMI rG'GwC_Cy
Cuts at: 0 480 582 690 1062 2110 3678 4525 4599
Size: 480 102 108 372 1048 1568 847 74
Cuts at: 4599 6105
Size: 1506
Fragments arranged by size:
1568 1506 1048 847 480 372 108 102
74
PshAI GACnn'nnGTC
Cuts at: 0 3859 6105
Size: 3859 2246
PspOMI G'GGCC_C
Cuts at: 0 391 2140 2510 3551 4482 5623 5675 5860
Size: 391 1749 370 1041 931 1141 52 185
Cuts at: 5860 6105
Size: 245
Fragments arranged by size:
1749 1141 1041 931 391 370 245 185
52
PstI C_TGCA'G
Cuts at: 0 47 682 1839 4562 4854 5102 5327 5334
Size: 47 635 1157 2723 292 248 225 7
Cuts at: 5334 5570 6002 6105
Size: 236 432 103
Fragments arranged by size:
2723 1157 635 432 292 248 236 225
103 47 7
PvuII CAG'CTG
Cuts at: 0 3166 3421 4539 4861 5097 5550 5835 6105
Size: 3166 255 1118 322 236 453 285 270
Fragments arranged by size:
3166 1118 453 322 285 270 255 236
SacI G_AGCT'C
Cuts at: 0 131 4628 5794 5813 5821 6105
Size: 131 4497 1166 19 8 284
Fragments arranged by size:
4497 1166 284 131 19 8
SapI GCTCTTCn'nnn_
Cuts at: 0 1987 6105
Size: 1987 4118
ScaI AGT'ACT
Cuts at: 0 5767 6105
Size: 5767 338
SexAI A'CCwGG_T
Cuts at: 0 1046 6105
Size: 1046 5059
SfcI C'TryA_G
Cuts at: 0 43 678 1835 2957 3362 4558 4850 5098
Size: 43 635 1157 1122 405 1196 292 248
Cuts at: 5098 5323 5330 5566 5998 6105
Size: 225 7 236 432 107
Fragments arranged by size:
1196 1157 1122 635 432 405 292 248
236 225 107 43 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1645 1654 3550 5836 6105
Size: 1645 9 1896 2286 269
Fragments arranged by size:
2286 1896 1645 269 9
SfoI GGC'GCC
Cuts at: 0 752 2346 3384 3694 6105
Size: 752 1594 1038 310 2411
Fragments arranged by size:
2411 1594 1038 752 310
SgrAI Cr'CCGG_yG
Cuts at: 0 4945 6105
Size: 4945 1160
SmaI CCC'GGG
Cuts at: 0 598 2516 3603 4481 6105
Size: 598 1918 1087 878 1624
Fragments arranged by size:
1918 1624 1087 878 598
SmlI C'TyrA_G
Cuts at: 0 1439 2503 2757 5812 6105
Size: 1439 1064 254 3055 293
Fragments arranged by size:
3055 1439 1064 293 254
SpeI A'CTAG_T
Cuts at: 0 2704 6105
Size: 2704 3401
SphI G_CATG'C
Cuts at: 0 1827 6105
Size: 1827 4278
StuI AGG'CCT
Cuts at: 0 1036 6105
Size: 1036 5069
StyI C'CwwG_G
Cuts at: 0 499 908 1736 2402 2549 2646 2735 3029
Size: 499 409 828 666 147 97 89 294
Cuts at: 3029 3108 3576 4955 6105
Size: 79 468 1379 1150
Fragments arranged by size:
1379 1150 828 666 499 468 409 294
147 97 89 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 230 807 1254 1681 1707 2350 2887 3145
Size: 230 577 447 427 26 643 537 258
Cuts at: 3145 3730 4003 4098 4108 4203 4390 4743 5359
Size: 585 273 95 10 95 187 353 616
Cuts at: 5359 5362 5415 6105
Size: 3 53 690
Fragments arranged by size:
690 643 616 585 577 537 447 427
353 273 258 230 187 95 95 53
26 10 3
TatI w'GTAC_w
Cuts at: 0 182 1001 1107 5765 5852 6105
Size: 182 819 106 4658 87 253
Fragments arranged by size:
4658 819 253 182 106 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 409 441 856 888 1002 1034 1428 1460
Size: 409 32 415 32 114 32 394 32
Cuts at: 1460 5416 5448 5468 5500 6105
Size: 3956 32 20 32 605
Fragments arranged by size:
3956 605 415 409 394 114 32 32
32 32 32 32 20
Tth111I GACn'n_nGTC
Cuts at: 0 4464 6105
Size: 4464 1641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1779 2733 3096 3288 3462 3537 4036 4578
Size: 1779 954 363 192 174 75 499 542
Cuts at: 4578 4803 5145 5259 5280 6105
Size: 225 342 114 21 825
Fragments arranged by size:
1779 954 825 542 499 363 342 225
192 174 114 75 21
XmaI C'CCGG_G
Cuts at: 0 596 2514 3601 4479 6105
Size: 596 1918 1087 878 1626
Fragments arranged by size:
1918 1626 1087 878 596
ZraI GAC'GTC
Cuts at: 0 840 6105
Size: 840 5265
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AvaI AvrII
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FseI FspI HaeII Hin4I KasI KpnI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PstI PvuII SacI SapI ScaI SexAI SfcI
SfiI SfoI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AloI ApoI AscI AseI AsiSI BaeI
BamHI BfrBI BglII BmtI BsiWI BspHI BsrBI BssHII
BstZ17I ClaI DraI EcoRI EcoRV FalI FspAI HincII
HindIII HpaI MluI NdeI NheI NruI NsiI PacI
PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SgfI SnaBI SrfI SspI
SwaI XbaI XhoI XmnI