Restriction map for
pFN21AE3098
(Linear) (Six-base) MAPSORT of: KIEE3098.seq Check: 9336 from: 1 to: 1305
KIEE3098.orf
With 182 enzymes: SgfI *
September 4, 2015 15:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1148 1182 1305
Size: 1148 34 123
Fragments arranged by size:
1148 123 34
AccI GT'mk_AC
Cuts at: 0 1286 1305
Size: 1286 19
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1153 1305
Size: 352 801 152
Fragments arranged by size:
801 352 152
AflIII A'CryG_T
Cuts at: 0 722 1305
Size: 722 583
AleI CACnn'nnGTG
Cuts at: 0 1000 1305
Size: 1000 305
AlwNI CAG_nnn'CTG
Cuts at: 0 625 1305
Size: 625 680
ApaI G_GGCC'C
Cuts at: 0 59 869 931 1275 1305
Size: 59 810 62 344 30
Fragments arranged by size:
810 344 62 59 30
ApaLI G'TGCA_C
Cuts at: 0 745 1305
Size: 745 560
ApoI r'AATT_y
Cuts at: 0 820 1305
Size: 820 485
AvaI C'yCGr_G
Cuts at: 0 34 44 58 826 930 1144 1305
Size: 34 10 14 768 104 214 161
Fragments arranged by size:
768 214 161 104 34 14 10
BanII G_rGCy'C
Cuts at: 0 59 109 602 854 869 931 1275 1305
Size: 59 50 493 252 15 62 344 30
Fragments arranged by size:
493 344 252 62 59 50 30 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 453 1236 1305
Size: 253 200 783 69
Fragments arranged by size:
783 253 200 69
BbvCI CC'TCA_GC
Cuts at: 0 618 1305
Size: 618 687
BciVI GTATCCnnnnn_n'
Cuts at: 0 1093 1305
Size: 1093 212
BclI T'GATC_A
Cuts at: 0 1112 1305
Size: 1112 193
BglI GCCn_nnn'nGGC
Cuts at: 0 642 1305
Size: 642 663
BglII A'GATC_T
Cuts at: 0 809 1305
Size: 809 496
BlpI GC'TnA_GC
Cuts at: 0 586 1305
Size: 586 719
Bme1580I G_kGCm'C
Cuts at: 0 59 749 869 931 1275 1305
Size: 59 690 120 62 344 30
Fragments arranged by size:
690 344 120 62 59 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 703 808 1078 1192 1305
Size: 504 199 105 270 114 113
Fragments arranged by size:
504 270 199 114 113 105
Bpu10I CC'TnA_GC
Cuts at: 0 618 1305
Size: 618 687
BsaI GGTCTCn'nnnn_
Cuts at: 0 354 707 1213 1305
Size: 354 353 506 92
Fragments arranged by size:
506 354 353 92
BsaAI yAC'GTr
Cuts at: 0 725 1305
Size: 725 580
BsaHI Gr'CG_yC
Cuts at: 0 4 379 1305
Size: 4 375 926
Fragments arranged by size:
926 375 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 334 364 1030 1060 1305
Size: 85 30 219 30 666 30 245
Fragments arranged by size:
666 245 219 85 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 654 1305
Size: 654 651
BseYI C'CCAG_C
Cuts at: 0 1274 1305
Size: 1274 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1305
Size: 727 578
BsiEI CG_ry'CG
Cuts at: 0 162 204 1305
Size: 162 42 1101
Fragments arranged by size:
1101 162 42
BsiHKAI G_wGCw'C
Cuts at: 0 749 854 1009 1268 1305
Size: 749 105 155 259 37
Fragments arranged by size:
749 259 155 105 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 408 996 1305
Size: 408 588 309
Fragments arranged by size:
588 408 309
Bsp1286I G_dGCh'C
Cuts at: 0 59 109 602 749 854 869 931 1009
Size: 59 50 493 147 105 15 62 78
Cuts at: 1009 1268 1275 1305
Size: 259 7 30
Fragments arranged by size:
493 259 147 105 78 62 59 50
30 15 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1148 1182 1305
Size: 1148 34 123
Fragments arranged by size:
1148 123 34
BsrBI CCG'CTC
Cuts at: 0 612 1091 1305
Size: 612 479 214
Fragments arranged by size:
612 479 214
BsrDI GCAATG_nn'
Cuts at: 0 656 1232 1305
Size: 656 576 73
Fragments arranged by size:
656 576 73
BsrFI r'CCGG_y
Cuts at: 0 161 1305
Size: 161 1144
BssSI C'ACGA_G
Cuts at: 0 1200 1305
Size: 1200 105
BstBI TT'CG_AA
Cuts at: 0 1247 1305
Size: 1247 58
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 1305
Size: 339 966
BstYI r'GATC_y
Cuts at: 0 809 1305
Size: 809 496
EaeI y'GGCC_r
Cuts at: 0 140 159 201 753 1305
Size: 140 19 42 552 552
Fragments arranged by size:
552 552 140 42 19
EagI C'GGCC_G
Cuts at: 0 159 201 1305
Size: 159 42 1104
Fragments arranged by size:
1104 159 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 180 1305
Size: 180 1125
EcoICRI GAG'CTC
Cuts at: 0 852 1305
Size: 852 453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 504 703 808 1078 1153 1192 1305
Size: 352 152 199 105 270 75 39 113
Fragments arranged by size:
352 270 199 152 113 105 75 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 70 343 1305
Size: 70 273 962
Fragments arranged by size:
962 273 70
EcoO109I rG'GnC_Cy
Cuts at: 0 63 135 865 866 927 1271 1305
Size: 63 72 730 1 61 344 34
Fragments arranged by size:
730 344 72 63 61 34 1
HaeII r_GCGC'y
Cuts at: 0 1071 1305
Size: 1071 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 1305
Size: 334 32 939
Fragments arranged by size:
939 334 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 1305
Size: 32 1273
MscI TGG'CCA
Cuts at: 0 142 1305
Size: 142 1163
MslI CAynn'nnrTG
Cuts at: 0 703 1000 1305
Size: 703 297 305
Fragments arranged by size:
703 305 297
MspA1I CmG'CkG
Cuts at: 0 14 622 1305
Size: 14 608 683
Fragments arranged by size:
683 608 14
NaeI GCC'GGC
Cuts at: 0 163 1305
Size: 163 1142
NgoMIV G'CCGG_C
Cuts at: 0 161 1305
Size: 161 1144
PflMI CCAn_nnn'nTGG
Cuts at: 0 14 1305
Size: 14 1291
PfoI T'CCnGG_A
Cuts at: 0 128 680 920 1305
Size: 128 552 240 385
Fragments arranged by size:
552 385 240 128
PspOMI G'GGCC_C
Cuts at: 0 55 865 927 1271 1305
Size: 55 810 62 344 34
Fragments arranged by size:
810 344 62 55 34
PstI C_TGCA'G
Cuts at: 0 422 1193 1305
Size: 422 771 112
Fragments arranged by size:
771 422 112
PvuII CAG'CTG
Cuts at: 0 14 622 1305
Size: 14 608 683
Fragments arranged by size:
683 608 14
SacI G_AGCT'C
Cuts at: 0 854 1305
Size: 854 451
SbfI CC_TGCA'GG
Cuts at: 0 1193 1305
Size: 1193 112
SexAI A'CCwGG_T
Cuts at: 0 314 344 1305
Size: 314 30 961
Fragments arranged by size:
961 314 30
SfcI C'TryA_G
Cuts at: 0 418 1189 1305
Size: 418 771 116
Fragments arranged by size:
771 418 116
SmaI CCC'GGG
Cuts at: 0 36 46 932 1305
Size: 36 10 886 373
Fragments arranged by size:
886 373 36 10
StyI C'CwwG_G
Cuts at: 0 353 998 1305
Size: 353 645 307
Fragments arranged by size:
645 353 307
TatI w'GTAC_w
Cuts at: 0 959 1106 1151 1305
Size: 959 147 45 154
Fragments arranged by size:
959 154 147 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 85 117 1305
Size: 85 32 1188
Fragments arranged by size:
1188 85 32
Tth111I GACn'n_nGTC
Cuts at: 0 346 1305
Size: 346 959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1191 1305
Size: 1191 114
XmaI C'CCGG_G
Cuts at: 0 34 44 930 1305
Size: 34 10 886 375
Fragments arranged by size:
886 375 34 10
XmnI GAAnn'nnTTC
Cuts at: 0 427 1305
Size: 427 878
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BanII BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstBI BstXI BstYI
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV PflMI
PfoI PspOMI PstI PvuII SacI SbfI SexAI SfcI
SmaI StyI TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BcgI BfrBI BmgBI BmrI BmtI BplI BpuEI
BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrGI BssHII
BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EarI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI ScaI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII XbaI XhoI ZraI