Restriction map for
pFN21AE3096
(Linear) (Six-base) MAPSORT of: KIEE3096.seq Check: 4097 from: 1 to: 801
KIEE3096.orf
With 182 enzymes: SgfI *
March 22, 2012 16:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 306 801
Size: 306 495
AccI GT'mk_AC
Cuts at: 0 746 801
Size: 746 55
Acc65I G'GTAC_C
Cuts at: 0 518 801
Size: 518 283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 801
Size: 250 551
AfeI AGC'GCT
Cuts at: 0 718 801
Size: 718 83
AlwNI CAG_nnn'CTG
Cuts at: 0 321 773 801
Size: 321 452 28
Fragments arranged by size:
452 321 28
ApaLI G'TGCA_C
Cuts at: 0 138 801
Size: 138 663
AvaI C'yCGr_G
Cuts at: 0 463 801
Size: 463 338
BanI G'GyrC_C
Cuts at: 0 188 208 518 801
Size: 188 20 310 283
Fragments arranged by size:
310 283 188 20
BanII G_rGCy'C
Cuts at: 0 22 151 801
Size: 22 129 650
Fragments arranged by size:
650 129 22
BbeI G_GCGC'C
Cuts at: 0 192 212 801
Size: 192 20 589
Fragments arranged by size:
589 192 20
BbvCI CC'TCA_GC
Cuts at: 0 36 801
Size: 36 765
BglI GCCn_nnn'nGGC
Cuts at: 0 175 801
Size: 175 626
Bme1580I G_kGCm'C
Cuts at: 0 142 801
Size: 142 659
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 703 801
Size: 703 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 801
Size: 673 128
Bpu10I CC'TnA_GC
Cuts at: 0 36 801
Size: 36 765
BsaAI yAC'GTr
Cuts at: 0 137 801
Size: 137 664
BsaHI Gr'CG_yC
Cuts at: 0 189 209 801
Size: 189 20 592
Fragments arranged by size:
592 189 20
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 801
Size: 343 30 428
Fragments arranged by size:
428 343 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 309 801
Size: 309 492
BseYI C'CCAG_C
Cuts at: 0 152 311 488 801
Size: 152 159 177 313
Fragments arranged by size:
313 177 159 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 696 801
Size: 635 61 105
Fragments arranged by size:
635 105 61
BsiEI CG_ry'CG
Cuts at: 0 255 801
Size: 255 546
BsiHKAI G_wGCw'C
Cuts at: 0 142 151 801
Size: 142 9 650
Fragments arranged by size:
650 142 9
Bsp1286I G_dGCh'C
Cuts at: 0 22 142 151 801
Size: 22 120 9 650
Fragments arranged by size:
650 120 22 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 306 801
Size: 306 495
BsrDI GCAATG_nn'
Cuts at: 0 541 801
Size: 541 260
BssSI C'ACGA_G
Cuts at: 0 697 801
Size: 697 104
BstAPI GCAn_nnn'nTGC
Cuts at: 0 321 801
Size: 321 480
BstEII G'GTnAC_C
Cuts at: 0 441 801
Size: 441 360
Bsu36I CC'TnA_GG
Cuts at: 0 7 292 801
Size: 7 285 509
Fragments arranged by size:
509 285 7
BtgI C'CryG_G
Cuts at: 0 157 801
Size: 157 644
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 495 801
Size: 495 306
BtsI GCAGTG_nn'
Cuts at: 0 236 246 801
Size: 236 10 555
Fragments arranged by size:
555 236 10
EaeI y'GGCC_r
Cuts at: 0 252 801
Size: 252 549
EagI C'GGCC_G
Cuts at: 0 252 801
Size: 252 549
EcoICRI GAG'CTC
Cuts at: 0 149 801
Size: 149 652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 673 801
Size: 250 423 128
Fragments arranged by size:
423 250 128
EcoO109I rG'GnC_Cy
Cuts at: 0 26 181 467 664 693 801
Size: 26 155 286 197 29 108
Fragments arranged by size:
286 197 155 108 29 26
HaeII r_GCGC'y
Cuts at: 0 192 212 720 801
Size: 192 20 508 81
Fragments arranged by size:
508 192 81 20
KasI G'GCGC_C
Cuts at: 0 188 208 801
Size: 188 20 593
Fragments arranged by size:
593 188 20
KpnI G_GTAC'C
Cuts at: 0 522 801
Size: 522 279
NarI GG'CG_CC
Cuts at: 0 189 209 801
Size: 189 20 592
Fragments arranged by size:
592 189 20
NotI GC'GGCC_GC
Cuts at: 0 252 801
Size: 252 549
PasI CC'CwG_GG
Cuts at: 0 276 627 801
Size: 276 351 174
Fragments arranged by size:
351 276 174
PfoI T'CCnGG_A
Cuts at: 0 361 801
Size: 361 440
PpuMI rG'GwC_Cy
Cuts at: 0 26 181 467 664 801
Size: 26 155 286 197 137
Fragments arranged by size:
286 197 155 137 26
PshAI GACnn'nnGTC
Cuts at: 0 644 801
Size: 644 157
PstI C_TGCA'G
Cuts at: 0 395 801
Size: 395 406
SacI G_AGCT'C
Cuts at: 0 151 801
Size: 151 650
SanDI GG'GwC_CC
Cuts at: 0 181 664 801
Size: 181 483 137
Fragments arranged by size:
483 181 137
SfcI C'TryA_G
Cuts at: 0 83 391 397 801
Size: 83 308 6 404
Fragments arranged by size:
404 308 83 6
SfoI GGC'GCC
Cuts at: 0 190 210 801
Size: 190 20 591
Fragments arranged by size:
591 190 20
SmaI CCC'GGG
Cuts at: 0 465 801
Size: 465 336
StuI AGG'CCT
Cuts at: 0 451 580 801
Size: 451 129 221
Fragments arranged by size:
451 221 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 325 460 801
Size: 325 135 341
Fragments arranged by size:
341 325 135
TatI w'GTAC_w
Cuts at: 0 764 801
Size: 764 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 59 91 553 585 656 688 801
Size: 59 32 462 32 71 32 113
Fragments arranged by size:
462 113 71 59 32 32 32
XmaI C'CCGG_G
Cuts at: 0 463 801
Size: 463 338
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AlwNI ApaLI AvaI
BanI BanII BbeI BbvCI BglI Bme1580I BplI BpmI
Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI BstEII Bsu36I
BtgI BtgZI BtsI EaeI EagI EcoICRI Eco57MI EcoO109I
HaeII KasI KpnI NarI NotI PasI PfoI PpuMI
PshAI PstI SacI SanDI SfcI SfoI SmaI StuI
TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AleI AlfI
AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstXI BstYI BstZ17I ClaI CspCI DraI DraIII
DrdI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StyI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI