Restriction map for
pFN21AE3094
(Linear) (Six-base) MAPSORT of: KIEE3094.seq Check: 5560 from: 1 to: 789
KIEE3094.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 282 760 789
Size: 280 2 478 29
Fragments arranged by size:
478 280 29 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 508 789
Size: 508 281
AlwNI CAG_nnn'CTG
Cuts at: 0 597 789
Size: 597 192
ApaI G_GGCC'C
Cuts at: 0 208 789
Size: 208 581
AvaI C'yCGr_G
Cuts at: 0 324 426 789
Size: 324 102 363
Fragments arranged by size:
363 324 102
BanI G'GyrC_C
Cuts at: 0 784 789
Size: 784 5
BanII G_rGCy'C
Cuts at: 0 87 171 208 683 789
Size: 87 84 37 475 106
Fragments arranged by size:
475 106 87 84 37
BbeI G_GCGC'C
Cuts at: 0 788 789
Size: 788 1
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 182 216 789
Size: 182 34 573
Fragments arranged by size:
573 182 34
BglI GCCn_nnn'nGGC
Cuts at: 0 194 472 789
Size: 194 278 317
Fragments arranged by size:
317 278 194
BlpI GC'TnA_GC
Cuts at: 0 223 789
Size: 223 566
Bme1580I G_kGCm'C
Cuts at: 0 208 789
Size: 208 581
BmgBI CAC'GTC
Cuts at: 0 501 789
Size: 501 288
BmrI ACTGGGnnnn_n'
Cuts at: 0 81 789
Size: 81 708
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 768 789
Size: 768 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 607 789
Size: 256 351 182
Fragments arranged by size:
351 256 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 104 145 172 789
Size: 104 41 27 617
Fragments arranged by size:
617 104 41 27
BsaAI yAC'GTr
Cuts at: 0 185 582 789
Size: 185 397 207
Fragments arranged by size:
397 207 185
BsaHI Gr'CG_yC
Cuts at: 0 785 789
Size: 785 4
BsaWI w'CCGG_w
Cuts at: 0 118 789
Size: 118 671
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 158 169 188 199 789
Size: 158 11 19 11 590
Fragments arranged by size:
590 158 19 11 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 352 637 789
Size: 34 318 285 152
Fragments arranged by size:
318 285 152 34
BseYI C'CCAG_C
Cuts at: 0 68 241 789
Size: 68 173 548
Fragments arranged by size:
548 173 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 490 562 583 789
Size: 481 9 72 21 206
Fragments arranged by size:
481 206 72 21 9
BsiHKAI G_wGCw'C
Cuts at: 0 171 253 281 789
Size: 171 82 28 508
Fragments arranged by size:
508 171 82 28
Bsp1286I G_dGCh'C
Cuts at: 0 87 171 208 253 281 683 789
Size: 87 84 37 45 28 402 106
Fragments arranged by size:
402 106 87 84 45 37 28
BspEI T'CCGG_A
Cuts at: 0 118 789
Size: 118 671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 515 789
Size: 515 274
BsrBI CCG'CTC
Cuts at: 0 227 789
Size: 227 562
BsrFI r'CCGG_y
Cuts at: 0 136 397 781 789
Size: 136 261 384 8
Fragments arranged by size:
384 261 136 8
BssSI C'ACGA_G
Cuts at: 0 315 444 789
Size: 315 129 345
Fragments arranged by size:
345 315 129
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 468 516 597 789
Size: 45 423 48 81 192
Fragments arranged by size:
423 192 81 48 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 216 789
Size: 189 27 573
Fragments arranged by size:
573 189 27
BstYI r'GATC_y
Cuts at: 0 128 789
Size: 128 661
BtsI GCAGTG_nn'
Cuts at: 0 574 789
Size: 574 215
EarI CTCTTCn'nnn_
Cuts at: 0 241 256 789
Size: 241 15 533
Fragments arranged by size:
533 241 15
EcoICRI GAG'CTC
Cuts at: 0 169 789
Size: 169 620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 280 282 607 760 789
Size: 256 24 2 325 153 29
Fragments arranged by size:
325 256 153 29 24 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 721 789
Size: 721 68
EcoO109I rG'GnC_Cy
Cuts at: 0 205 789
Size: 205 584
HaeII r_GCGC'y
Cuts at: 0 751 788 789
Size: 751 37 1
Fragments arranged by size:
751 37 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 789
Size: 144 32 613
Fragments arranged by size:
613 144 32
KasI G'GCGC_C
Cuts at: 0 784 789
Size: 784 5
MslI CAynn'nnrTG
Cuts at: 0 490 559 789
Size: 490 69 230
Fragments arranged by size:
490 230 69
MspA1I CmG'CkG
Cuts at: 0 284 594 789
Size: 284 310 195
Fragments arranged by size:
310 284 195
NaeI GCC'GGC
Cuts at: 0 138 399 789
Size: 138 261 390
Fragments arranged by size:
390 261 138
NarI GG'CG_CC
Cuts at: 0 785 789
Size: 785 4
NgoMIV G'CCGG_C
Cuts at: 0 136 397 789
Size: 136 261 392
Fragments arranged by size:
392 261 136
NspI r_CATG'y
Cuts at: 0 201 418 789
Size: 201 217 371
Fragments arranged by size:
371 217 201
PfoI T'CCnGG_A
Cuts at: 0 53 272 789
Size: 53 219 517
Fragments arranged by size:
517 219 53
PmlI CAC'GTG
Cuts at: 0 185 582 789
Size: 185 397 207
Fragments arranged by size:
397 207 185
PspOMI G'GGCC_C
Cuts at: 0 204 789
Size: 204 585
PstI C_TGCA'G
Cuts at: 0 512 789
Size: 512 277
SacI G_AGCT'C
Cuts at: 0 171 789
Size: 171 618
SapI GCTCTTCn'nnn_
Cuts at: 0 241 789
Size: 241 548
SbfI CC_TGCA'GG
Cuts at: 0 512 789
Size: 512 277
SfcI C'TryA_G
Cuts at: 0 508 789
Size: 508 281
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 472 789
Size: 472 317
SfoI GGC'GCC
Cuts at: 0 786 789
Size: 786 3
SgrAI Cr'CCGG_yG
Cuts at: 0 136 789
Size: 136 653
SphI G_CATG'C
Cuts at: 0 418 789
Size: 418 371
StuI AGG'CCT
Cuts at: 0 213 727 789
Size: 213 514 62
Fragments arranged by size:
514 213 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 169 190 201 789
Size: 158 11 21 11 588
Fragments arranged by size:
588 158 21 11 11
Enzymes that do cut:
AcuI AlfI AlwNI ApaI AvaI BanI BanII BbeI
BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI
BstXI BstYI BtsI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV
NspI PfoI PmlI PspOMI PstI SacI SapI SbfI
SfcI SfiI SfoI SgrAI SphI StuI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI
BfrBI BglII BmtI Bpu10I BpuEI BsaBI BsiEI BsiWI
BsmI BsmBI BspHI BsrDI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI ScaI SexAI SgfI SmaI SmlI SnaBI SpeI
SrfI SspI StyI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI