(Linear) (Six-base) MAPSORT of: KIEE3090.seq  Check: 4193  from: 1  to: 780



KIEE3090.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     780 
   Size:        229     551
 
ApoI r'AATT_y

Cuts at:      0     199     780 
   Size:        199     581
 
BanI G'GyrC_C

Cuts at:      0     690     780 
   Size:        690      90
 
BanII G_rGCy'C

Cuts at:      0     321     780 
   Size:        321     459
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     725     780 
   Size:        725      55
 
BfrBI ATG'CAT

Cuts at:      0     437     780 
   Size:        437     343
 
BlpI GC'TnA_GC

Cuts at:      0     320     780 
   Size:        320     460
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     527     780 
   Size:        527     253
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     176     780 
   Size:        176     604
 
BsiHKAI G_wGCw'C

Cuts at:      0     321     780 
   Size:        321     459
 
BsmI GAATG_Cn'

Cuts at:      0     439     596     780 
   Size:        439     157     184

  Fragments arranged by size: 

                 439     184     157
 
Bsp1286I G_dGCh'C

Cuts at:      0     321     780 
   Size:        321     459
 
BsrDI GCAATG_nn'

Cuts at:      0     183     780 
   Size:        183     597
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      72     780 
   Size:         72     708
 
DraI TTT'AAA

Cuts at:      0     726     780 
   Size:        726      54
 
EaeI y'GGCC_r

Cuts at:      0       2     498     780 
   Size:          2     496     282

  Fragments arranged by size: 

                 496     282       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     581     780 
   Size:        581     199
 
EcoICRI GAG'CTC

Cuts at:      0     319     780 
   Size:        319     461
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     527     780 
   Size:        229     298     253

  Fragments arranged by size: 

                 298     253     229
 
EcoO109I rG'GnC_Cy

Cuts at:      0      53     780 
   Size:         53     727
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     447     479     780 
   Size:        447      32     301

  Fragments arranged by size: 

                 447     301      32
 
HincII GTy'rAC

Cuts at:      0     104     780 
   Size:        104     676
 
HindIII A'AGCT_T

Cuts at:      0     619     780 
   Size:        619     161
 
MfeI C'AATT_G

Cuts at:      0     127     780 
   Size:        127     653
 
MscI TGG'CCA

Cuts at:      0       4     500     780 
   Size:          4     496     280

  Fragments arranged by size: 

                 496     280       4
 
NdeI CA'TA_TG

Cuts at:      0     294     780 
   Size:        294     486
 
NsiI A_TGCA'T

Cuts at:      0     439     780 
   Size:        439     341
 
NspI r_CATG'y

Cuts at:      0     554     780 
   Size:        554     226
 
PpuMI rG'GwC_Cy

Cuts at:      0      53     780 
   Size:         53     727
 
PsiI TTA'TAA

Cuts at:      0     629     780 
   Size:        629     151
 
PstI C_TGCA'G

Cuts at:      0      96     780 
   Size:         96     684
 
SacI G_AGCT'C

Cuts at:      0     321     780 
   Size:        321     459
 
SanDI GG'GwC_CC

Cuts at:      0      53     780 
   Size:         53     727
 
SapI GCTCTTCn'nnn_

Cuts at:      0     581     780 
   Size:        581     199
 
ScaI AGT'ACT

Cuts at:      0     189     774     780 
   Size:        189     585       6

  Fragments arranged by size: 

                 585     189       6
 
SfcI C'TryA_G

Cuts at:      0      92     780 
   Size:         92     688
 
SmlI C'TyrA_G

Cuts at:      0     191     780 
   Size:        191     589
 
SspI AAT'ATT

Cuts at:      0     610     780 
   Size:        610     170
 
TatI w'GTAC_w

Cuts at:      0     187     772     780 
   Size:        187     585       8

  Fragments arranged by size: 

                 585     187       8
 
XmnI GAAnn'nnTTC

Cuts at:      0     149     780 
   Size:        149     631
 

 Enzymes that do cut:

     AcuI     ApoI     BanI    BanII     BbsI    BfrBI     BlpI     BpmI
    BpuEI  BsiHKAI     BsmI Bsp1286I    BsrDI    BtgZI     DraI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    Hin4I   HincII  HindIII     MfeI
     MscI     NdeI     NsiI     NspI    PpuMI     PsiI     PstI     SacI
    SanDI     SapI     ScaI     SfcI     SmlI     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MluI     MmeI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII    SacII     SalI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI