Restriction map for
pFN21AE3082
(Linear) (Six-base) MAPSORT of: KIEE3082.seq Check: 4121 from: 1 to: 729
KIEE3082.orf
With 182 enzymes: SgfI *
March 22, 2012 16:12 ..
AccI GT'mk_AC
Cuts at: 0 521 729
Size: 521 208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 729
Size: 251 478
ApoI r'AATT_y
Cuts at: 0 45 196 729
Size: 45 151 533
Fragments arranged by size:
533 151 45
BanI G'GyrC_C
Cuts at: 0 142 729
Size: 142 587
BanII G_rGCy'C
Cuts at: 0 29 269 729
Size: 29 240 460
Fragments arranged by size:
460 240 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 124 158 729
Size: 124 34 571
Fragments arranged by size:
571 124 34
BclI T'GATC_A
Cuts at: 0 282 729
Size: 282 447
BmrI ACTGGGnnnn_n'
Cuts at: 0 50 382 729
Size: 50 332 347
Fragments arranged by size:
347 332 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 729
Size: 583 146
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 694 729
Size: 694 35
BseYI C'CCAG_C
Cuts at: 0 385 729
Size: 385 344
BsiHKAI G_wGCw'C
Cuts at: 0 269 729
Size: 269 460
Bsp1286I G_dGCh'C
Cuts at: 0 29 269 729
Size: 29 240 460
Fragments arranged by size:
460 240 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 497 570 729
Size: 497 73 159
Fragments arranged by size:
497 159 73
BstAPI GCAn_nnn'nTGC
Cuts at: 0 513 729
Size: 513 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 707 729
Size: 707 22
BtgI C'CryG_G
Cuts at: 0 713 729
Size: 713 16
EaeI y'GGCC_r
Cuts at: 0 716 729
Size: 716 13
EcoICRI GAG'CTC
Cuts at: 0 267 729
Size: 267 462
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 729
Size: 251 478
EcoRI G'AATT_C
Cuts at: 0 196 729
Size: 196 533
EcoRV GAT'ATC
Cuts at: 0 162 729
Size: 162 567
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 251 283 729
Size: 251 32 446
Fragments arranged by size:
446 251 32
HindIII A'AGCT_T
Cuts at: 0 183 729
Size: 183 546
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 519 543 729
Size: 2 517 24 186
Fragments arranged by size:
517 186 24 2
MscI TGG'CCA
Cuts at: 0 718 729
Size: 718 11
MslI CAynn'nnrTG
Cuts at: 0 529 729
Size: 529 200
NcoI C'CATG_G
Cuts at: 0 713 729
Size: 713 16
NspI r_CATG'y
Cuts at: 0 10 520 729
Size: 10 510 209
Fragments arranged by size:
510 209 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 405 729
Size: 405 324
PsiI TTA'TAA
Cuts at: 0 460 729
Size: 460 269
SacI G_AGCT'C
Cuts at: 0 269 729
Size: 269 460
ScaI AGT'ACT
Cuts at: 0 55 648 729
Size: 55 593 81
Fragments arranged by size:
593 81 55
SmlI C'TyrA_G
Cuts at: 0 598 729
Size: 598 131
SphI G_CATG'C
Cuts at: 0 10 729
Size: 10 719
StuI AGG'CCT
Cuts at: 0 228 380 596 729
Size: 228 152 216 133
Fragments arranged by size:
228 216 152 133
StyI C'CwwG_G
Cuts at: 0 713 729
Size: 713 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 713 729
Size: 713 16
TatI w'GTAC_w
Cuts at: 0 53 646 729
Size: 53 593 83
Fragments arranged by size:
593 83 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 435 467 729
Size: 435 32 262
Fragments arranged by size:
435 262 32
Enzymes that do cut:
AccI AcuI ApoI BanI BanII BcgI BclI BmrI
BpuEI BseRI BseYI BsiHKAI Bsp1286I BspMI BstAPI BstXI
BtgI EaeI EcoICRI Eco57MI EcoRI EcoRV Hin4I HindIII
MmeI MscI MslI NcoI NspI PflMI PsiI SacI
ScaI SmlI SphI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoNI EcoO109I
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI