Restriction map for
pFN21AE3078
(Linear) (Six-base) MAPSORT of: KIEE3078.seq Check: 7437 from: 1 to: 714
KIEE3078.orf
With 182 enzymes: SgfI *
July 3, 2012 17:21 ..
AccI GT'mk_AC
Cuts at: 0 458 714
Size: 458 256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 561 667 714
Size: 54 507 106 47
Fragments arranged by size:
507 106 54 47
AlwNI CAG_nnn'CTG
Cuts at: 0 645 678 714
Size: 645 33 36
Fragments arranged by size:
645 36 33
ApaI G_GGCC'C
Cuts at: 0 518 714
Size: 518 196
ApoI r'AATT_y
Cuts at: 0 219 291 597 714
Size: 219 72 306 117
Fragments arranged by size:
306 219 117 72
BanII G_rGCy'C
Cuts at: 0 412 518 714
Size: 412 106 196
Fragments arranged by size:
412 196 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 455 714
Size: 455 259
BbvCI CC'TCA_GC
Cuts at: 0 145 714
Size: 145 569
Bme1580I G_kGCm'C
Cuts at: 0 518 714
Size: 518 196
BmrI ACTGGGnnnn_n'
Cuts at: 0 305 714
Size: 305 409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 714
Size: 43 671
Bpu10I CC'TnA_GC
Cuts at: 0 145 714
Size: 145 569
BsaHI Gr'CG_yC
Cuts at: 0 32 714
Size: 32 682
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 169 714
Size: 40 129 545
Fragments arranged by size:
545 129 40
Bsp1286I G_dGCh'C
Cuts at: 0 412 518 714
Size: 412 106 196
Fragments arranged by size:
412 196 106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 506 714
Size: 506 208
BsrBI CCG'CTC
Cuts at: 0 98 714
Size: 98 616
BsrDI GCAATG_nn'
Cuts at: 0 324 714
Size: 324 390
BssSI C'ACGA_G
Cuts at: 0 397 714
Size: 397 317
EaeI y'GGCC_r
Cuts at: 0 102 714
Size: 102 612
EarI CTCTTCn'nnn_
Cuts at: 0 643 714
Size: 643 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 54 561 667 714
Size: 43 11 507 106 47
Fragments arranged by size:
507 106 47 43 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 69 694 714
Size: 25 44 625 20
Fragments arranged by size:
625 44 25 20
EcoO109I rG'GnC_Cy
Cuts at: 0 59 378 514 714
Size: 59 319 136 200
Fragments arranged by size:
319 200 136 59
EcoRI G'AATT_C
Cuts at: 0 219 714
Size: 219 495
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 231 714
Size: 231 483
FspI TGC'GCA
Cuts at: 0 607 714
Size: 607 107
HaeII r_GCGC'y
Cuts at: 0 82 657 714
Size: 82 575 57
Fragments arranged by size:
575 82 57
MspA1I CmG'CkG
Cuts at: 0 8 636 714
Size: 8 628 78
Fragments arranged by size:
628 78 8
PasI CC'CwG_GG
Cuts at: 0 84 714
Size: 84 630
PflMI CCAn_nnn'nTGG
Cuts at: 0 295 714
Size: 295 419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 476 508 714
Size: 476 32 206
Fragments arranged by size:
476 206 32
PpuMI rG'GwC_Cy
Cuts at: 0 59 714
Size: 59 655
PsiI TTA'TAA
Cuts at: 0 431 714
Size: 431 283
PspOMI G'GGCC_C
Cuts at: 0 514 714
Size: 514 200
PstI C_TGCA'G
Cuts at: 0 641 714
Size: 641 73
PvuII CAG'CTG
Cuts at: 0 636 714
Size: 636 78
SfcI C'TryA_G
Cuts at: 0 12 592 637 666 714
Size: 12 580 45 29 48
Fragments arranged by size:
580 48 45 29 12
TatI w'GTAC_w
Cuts at: 0 252 714
Size: 252 462
Tth111I GACn'n_nGTC
Cuts at: 0 454 714
Size: 454 260
Enzymes that do cut:
AccI AcuI AlwNI ApaI ApoI BanII BbsI BbvCI
Bme1580I BmrI BpmI Bpu10I BsaHI BseRI Bsp1286I BspMI
BsrBI BsrDI BssSI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII MspA1I PasI PflMI PpiI
PpuMI PsiI PspOMI PstI PvuII SfcI TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRV FseI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PshAI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI XbaI XcmI
XhoI XmaI XmnI ZraI