Restriction map for
pFN21AE3077
(Linear) (Six-base) MAPSORT of: KIEE3077.seq Check: 7040 from: 1 to: 714
KIEE3077.orf
With 182 enzymes: SgfI *
March 22, 2012 16:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 416 714
Size: 207 209 298
Fragments arranged by size:
298 209 207
AgeI A'CCGG_T
Cuts at: 0 69 714
Size: 69 645
AleI CACnn'nnGTG
Cuts at: 0 94 714
Size: 94 620
ApoI r'AATT_y
Cuts at: 0 187 714
Size: 187 527
AvaI C'yCGr_G
Cuts at: 0 43 714
Size: 43 671
BanI G'GyrC_C
Cuts at: 0 245 714
Size: 245 469
BanII G_rGCy'C
Cuts at: 0 401 714
Size: 401 313
BfrBI ATG'CAT
Cuts at: 0 278 714
Size: 278 436
BglI GCCn_nnn'nGGC
Cuts at: 0 517 714
Size: 517 197
BmrI ACTGGGnnnn_n'
Cuts at: 0 129 714
Size: 129 585
BsaWI w'CCGG_w
Cuts at: 0 69 83 714
Size: 69 14 631
Fragments arranged by size:
631 69 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 424 444 474 530 560 714
Size: 394 30 20 30 56 30 154
Fragments arranged by size:
394 154 56 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 676 714
Size: 673 3 38
Fragments arranged by size:
673 38 3
BseYI C'CCAG_C
Cuts at: 0 5 414 714
Size: 5 409 300
Fragments arranged by size:
409 300 5
Bsp1286I G_dGCh'C
Cuts at: 0 401 714
Size: 401 313
BspEI T'CCGG_A
Cuts at: 0 83 714
Size: 83 631
BsrFI r'CCGG_y
Cuts at: 0 69 714
Size: 69 645
BstXI CCAn_nnnn'nTGG
Cuts at: 0 558 714
Size: 558 156
BtsI GCAGTG_nn'
Cuts at: 0 385 714
Size: 385 329
DraIII CAC_nnn'GTG
Cuts at: 0 316 714
Size: 316 398
EaeI y'GGCC_r
Cuts at: 0 128 714
Size: 128 586
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 284 577 714
Size: 284 293 137
Fragments arranged by size:
293 284 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 416 714
Size: 207 209 298
Fragments arranged by size:
298 209 207
EcoNI CCTnn'n_nnAGG
Cuts at: 0 61 714
Size: 61 653
EcoO109I rG'GnC_Cy
Cuts at: 0 33 546 714
Size: 33 513 168
Fragments arranged by size:
513 168 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 276 308 530 562 589 621
Size: 37 32 207 32 222 32 27 32
Cuts at: 621 714
Size: 93
Fragments arranged by size:
222 207 93 37 32 32 32 32
27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 714
Size: 131 583
MscI TGG'CCA
Cuts at: 0 130 714
Size: 130 584
MslI CAynn'nnrTG
Cuts at: 0 94 714
Size: 94 620
NsiI A_TGCA'T
Cuts at: 0 280 714
Size: 280 434
PflMI CCAn_nnn'nTGG
Cuts at: 0 242 316 714
Size: 242 74 398
Fragments arranged by size:
398 242 74
PpuMI rG'GwC_Cy
Cuts at: 0 546 714
Size: 546 168
PspXI vC'TCGA_Gb
Cuts at: 0 43 714
Size: 43 671
PstI C_TGCA'G
Cuts at: 0 62 714
Size: 62 652
SbfI CC_TGCA'GG
Cuts at: 0 62 714
Size: 62 652
SfcI C'TryA_G
Cuts at: 0 58 299 714
Size: 58 241 415
Fragments arranged by size:
415 241 58
SmlI C'TyrA_G
Cuts at: 0 43 714
Size: 43 671
XhoI C'TCGA_G
Cuts at: 0 43 714
Size: 43 671
Enzymes that do cut:
AcuI AgeI AleI ApoI AvaI BanI BanII BfrBI
BglI BmrI BsaWI BsaXI BseRI BseYI Bsp1286I BspEI
BsrFI BstXI BtsI DraIII EaeI EciI Eco57MI EcoNI
EcoO109I Hin4I MmeI MscI MslI NsiI PflMI PpuMI
PspXI PstI SbfI SfcI SmlI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI
EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI