Restriction map for
pFN21AE3076
(Linear) (Six-base) MAPSORT of: KIEE3076.seq Check: 3473 from: 1 to: 711
KIEE3076.orf
With 182 enzymes: SgfI *
March 22, 2012 16:11 ..
AatII G_ACGT'C
Cuts at: 0 356 711
Size: 356 355
Acc65I G'GTAC_C
Cuts at: 0 175 711
Size: 175 536
AclI AA'CG_TT
Cuts at: 0 288 711
Size: 288 423
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 215 711
Size: 201 14 496
Fragments arranged by size:
496 201 14
AlwNI CAG_nnn'CTG
Cuts at: 0 666 711
Size: 666 45
ApoI r'AATT_y
Cuts at: 0 502 711
Size: 502 209
BamHI G'GATC_C
Cuts at: 0 392 711
Size: 392 319
BanI G'GyrC_C
Cuts at: 0 175 480 711
Size: 175 305 231
Fragments arranged by size:
305 231 175
BanII G_rGCy'C
Cuts at: 0 71 711
Size: 71 640
BbsI GAAGACnn'nnnn_
Cuts at: 0 232 333 711
Size: 232 101 378
Fragments arranged by size:
378 232 101
Bme1580I G_kGCm'C
Cuts at: 0 422 485 711
Size: 422 63 226
Fragments arranged by size:
422 226 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 711
Size: 90 621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 688 711
Size: 85 603 23
Fragments arranged by size:
603 85 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 711
Size: 483 228
BsaAI yAC'GTr
Cuts at: 0 93 711
Size: 93 618
BsaBI GATnn'nnATC
Cuts at: 0 103 711
Size: 103 608
BsaHI Gr'CG_yC
Cuts at: 0 58 353 711
Size: 58 295 358
Fragments arranged by size:
358 295 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 679 711
Size: 115 564 32
Fragments arranged by size:
564 115 32
BsiEI CG_ry'CG
Cuts at: 0 47 711
Size: 47 664
BsiHKAI G_wGCw'C
Cuts at: 0 71 711
Size: 71 640
BsmI GAATG_Cn'
Cuts at: 0 406 711
Size: 406 305
Bsp1286I G_dGCh'C
Cuts at: 0 71 422 485 711
Size: 71 351 63 226
Fragments arranged by size:
351 226 71 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 157 268 711
Size: 157 111 443
Fragments arranged by size:
443 157 111
BsrDI GCAATG_nn'
Cuts at: 0 411 711
Size: 411 300
BstAPI GCAn_nnn'nTGC
Cuts at: 0 149 417 711
Size: 149 268 294
Fragments arranged by size:
294 268 149
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 711
Size: 210 501
BstYI r'GATC_y
Cuts at: 0 392 473 711
Size: 392 81 238
Fragments arranged by size:
392 238 81
BtgI C'CryG_G
Cuts at: 0 21 230 335 711
Size: 21 209 105 376
Fragments arranged by size:
376 209 105 21
EaeI y'GGCC_r
Cuts at: 0 44 542 711
Size: 44 498 169
Fragments arranged by size:
498 169 44
EagI C'GGCC_G
Cuts at: 0 44 711
Size: 44 667
EcoICRI GAG'CTC
Cuts at: 0 69 711
Size: 69 642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 201 215 688 711
Size: 85 116 14 473 23
Fragments arranged by size:
473 116 85 23 14
EcoO109I rG'GnC_Cy
Cuts at: 0 549 711
Size: 549 162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 477 711
Size: 477 234
KpnI G_GTAC'C
Cuts at: 0 179 711
Size: 179 532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 512 711
Size: 158 354 199
Fragments arranged by size:
354 199 158
MslI CAynn'nnrTG
Cuts at: 0 208 361 711
Size: 208 153 350
Fragments arranged by size:
350 208 153
MspA1I CmG'CkG
Cuts at: 0 49 711
Size: 49 662
NcoI C'CATG_G
Cuts at: 0 21 711
Size: 21 690
NspI r_CATG'y
Cuts at: 0 334 415 525 711
Size: 334 81 110 186
Fragments arranged by size:
334 186 110 81
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 711
Size: 570 141
PpuMI rG'GwC_Cy
Cuts at: 0 549 711
Size: 549 162
PstI C_TGCA'G
Cuts at: 0 35 711
Size: 35 676
SacI G_AGCT'C
Cuts at: 0 71 711
Size: 71 640
SfcI C'TryA_G
Cuts at: 0 31 711
Size: 31 680
SmlI C'TyrA_G
Cuts at: 0 462 711
Size: 462 249
SpeI A'CTAG_T
Cuts at: 0 426 711
Size: 426 285
SphI G_CATG'C
Cuts at: 0 415 711
Size: 415 296
StyI C'CwwG_G
Cuts at: 0 21 545 711
Size: 21 524 166
Fragments arranged by size:
524 166 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 403 441 711
Size: 207 196 38 270
Fragments arranged by size:
270 207 196 38
ZraI GAC'GTC
Cuts at: 0 354 711
Size: 354 357
Enzymes that do cut:
AatII Acc65I AclI AcuI AlwNI ApoI BamHI BanI
BanII BbsI Bme1580I BplI BpmI BpuEI BsaAI BsaBI
BsaHI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BstAPI BstXI BstYI BtgI EaeI EagI EcoICRI Eco57MI
EcoO109I FalI KpnI MmeI MslI MspA1I NcoI NspI
PflMI PpuMI PstI SacI SfcI SmlI SpeI SphI
StyI XcmI ZraI
Enzymes that do not cut:
AarI AccI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI Bpu10I BsaI
BsaWI BsaXI BseRI BseYI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI