Restriction map for
pFN21AE3073
(Linear) (Six-base) MAPSORT of: KIEE3073.seq Check: 2196 from: 1 to: 702
KIEE3073.orf
With 182 enzymes: SgfI *
March 22, 2012 16:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 702
Size: 376 326
AflIII A'CryG_T
Cuts at: 0 228 617 702
Size: 228 389 85
Fragments arranged by size:
389 228 85
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 702
Size: 586 116
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 702
Size: 265 32 405
Fragments arranged by size:
405 265 32
AlwNI CAG_nnn'CTG
Cuts at: 0 402 558 702
Size: 402 156 144
Fragments arranged by size:
402 156 144
BanII G_rGCy'C
Cuts at: 0 509 702
Size: 509 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 358 392 702
Size: 358 34 310
Fragments arranged by size:
358 310 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 591 702
Size: 591 111
BglII A'GATC_T
Cuts at: 0 209 702
Size: 209 493
BmgBI CAC'GTC
Cuts at: 0 416 702
Size: 416 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 702
Size: 202 500
Bpu10I CC'TnA_GC
Cuts at: 0 559 702
Size: 559 143
BsaAI yAC'GTr
Cuts at: 0 585 618 702
Size: 585 33 84
Fragments arranged by size:
585 84 33
BsaBI GATnn'nnATC
Cuts at: 0 475 702
Size: 475 227
BsaWI w'CCGG_w
Cuts at: 0 83 702
Size: 83 619
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 702
Size: 265 30 407
Fragments arranged by size:
407 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 286 481 702
Size: 286 195 221
Fragments arranged by size:
286 221 195
BseYI C'CCAG_C
Cuts at: 0 192 702
Size: 192 510
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 474 702
Size: 415 59 228
Fragments arranged by size:
415 228 59
BsiHKAI G_wGCw'C
Cuts at: 0 278 702
Size: 278 424
Bsp1286I G_dGCh'C
Cuts at: 0 278 509 702
Size: 278 231 193
Fragments arranged by size:
278 231 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 659 702
Size: 659 43
BsrDI GCAATG_nn'
Cuts at: 0 563 702
Size: 563 139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 402 702
Size: 402 300
BstYI r'GATC_y
Cuts at: 0 209 702
Size: 209 493
Bsu36I CC'TnA_GG
Cuts at: 0 626 702
Size: 626 76
BtgI C'CryG_G
Cuts at: 0 509 515 702
Size: 509 6 187
Fragments arranged by size:
509 187 6
DraIII CAC_nnn'GTG
Cuts at: 0 393 521 618 702
Size: 393 128 97 84
Fragments arranged by size:
393 128 97 84
EaeI y'GGCC_r
Cuts at: 0 512 702
Size: 512 190
EarI CTCTTCn'nnn_
Cuts at: 0 193 702
Size: 193 509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 376 702
Size: 202 174 326
Fragments arranged by size:
326 202 174
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 426 702
Size: 355 71 276
Fragments arranged by size:
355 276 71
EcoO109I rG'GnC_Cy
Cuts at: 0 161 555 702
Size: 161 394 147
Fragments arranged by size:
394 161 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 702
Size: 31 32 639
Fragments arranged by size:
639 32 31
MluI A'CGCG_T
Cuts at: 0 228 702
Size: 228 474
MscI TGG'CCA
Cuts at: 0 514 702
Size: 514 188
NcoI C'CATG_G
Cuts at: 0 509 702
Size: 509 193
PmlI CAC'GTG
Cuts at: 0 618 702
Size: 618 84
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 702
Size: 265 32 405
Fragments arranged by size:
405 265 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 139 171 702
Size: 139 32 531
Fragments arranged by size:
531 139 32
PstI C_TGCA'G
Cuts at: 0 239 702
Size: 239 463
SapI GCTCTTCn'nnn_
Cuts at: 0 193 702
Size: 193 509
SfcI C'TryA_G
Cuts at: 0 156 235 702
Size: 156 79 467
Fragments arranged by size:
467 156 79
StyI C'CwwG_G
Cuts at: 0 164 509 702
Size: 164 345 193
Fragments arranged by size:
345 193 164
XmnI GAAnn'nnTTC
Cuts at: 0 151 361 702
Size: 151 210 341
Fragments arranged by size:
341 210 151
Enzymes that do cut:
AcuI AflIII AlfI AloI AlwNI BanII BcgI BciVI
BglII BmgBI BpmI Bpu10I BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BstAPI
BstYI Bsu36I BtgI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I Hin4I MluI MscI NcoI PmlI PpiI PsrI
PstI SapI SfcI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BclI BfrBI BglI BlpI Bme1580I BmrI BmtI BplI
BpuEI BsaI BsaHI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MmeI MslI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpuMI PshAI PsiI PspOMI PspXI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI