(Linear) (Six-base) MAPSORT of: KIEE3069.seq  Check: 278  from: 1  to: 681



KIEE3069.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:09  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     681 
   Size:        226     455
 
AflIII A'CryG_T

Cuts at:      0      86     681 
   Size:         86     595
 
AhdI GACnn_n'nnGTC

Cuts at:      0     582     681 
   Size:        582      99
 
AseI AT'TA_AT

Cuts at:      0     533     681 
   Size:        533     148
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     449     681 
   Size:        449     232
 
BclI T'GATC_A

Cuts at:      0      69     681 
   Size:         69     612
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     681 
   Size:        625      56
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     639     669     681 
   Size:        639      30      12

  Fragments arranged by size: 

                 639      30      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      54     641     644     681 
   Size:         54     587       3      37

  Fragments arranged by size: 

                 587      54      37       3
 
BsmI GAATG_Cn'

Cuts at:      0     172     681 
   Size:        172     509
 
BsrBI CCG'CTC

Cuts at:      0     450     681 
   Size:        450     231
 
BssSI C'ACGA_G

Cuts at:      0     633     681 
   Size:        633      48
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      24     681 
   Size:         24     657
 
BtgI C'CryG_G

Cuts at:      0     308     681 
   Size:        308     373
 
BtsI GCAGTG_nn'

Cuts at:      0     123     614     681 
   Size:        123     491      67

  Fragments arranged by size: 

                 491     123      67
 
ClaI AT'CG_AT

Cuts at:      0     194     681 
   Size:        194     487
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     451     681 
   Size:        451     230
 
EaeI y'GGCC_r

Cuts at:      0      51     681 
   Size:         51     630
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     681 
   Size:        226     455
 
EcoRV GAT'ATC

Cuts at:      0     567     681 
   Size:        567     114
 
HaeII r_GCGC'y

Cuts at:      0      16     558     681 
   Size:         16     542     123

  Fragments arranged by size: 

                 542     123      16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     568     600     681 
   Size:        568      32      81

  Fragments arranged by size: 

                 568      81      32
 
HincII GTy'rAC

Cuts at:      0     374     475     681 
   Size:        374     101     206

  Fragments arranged by size: 

                 374     206     101
 
HindIII A'AGCT_T

Cuts at:      0     238     681 
   Size:        238     443
 
MslI CAynn'nnrTG

Cuts at:      0      22     681 
   Size:         22     659
 
NcoI C'CATG_G

Cuts at:      0     308     681 
   Size:        308     373
 
NspI r_CATG'y

Cuts at:      0      90     681 
   Size:         90     591
 
PciI A'CATG_T

Cuts at:      0      86     681 
   Size:         86     595
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      67      99     681 
   Size:         67      32     582

  Fragments arranged by size: 

                 582      67      32
 
SmlI C'TyrA_G

Cuts at:      0     640     681 
   Size:        640      41
 
StyI C'CwwG_G

Cuts at:      0     308     521     681 
   Size:        308     213     160

  Fragments arranged by size: 

                 308     213     160
 
TatI w'GTAC_w

Cuts at:      0     360     681 
   Size:        360     321
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     417     449     681 
   Size:        417      32     232

  Fragments arranged by size: 

                 417     232      32
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AseI     BbsI     BclI    BpuEI    BsaXI
    BseRI     BsmI    BsrBI    BssSI    BstXI     BtgI     BtsI     ClaI
     DrdI     EaeI  Eco57MI    EcoRV    HaeII    Hin4I   HincII  HindIII
     MslI     NcoI     NspI     PciI     PsrI     SmlI     StyI     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI