Restriction map for
pFN21AE3067
(Linear) (Six-base) MAPSORT of: KIEE3067.seq Check: 3122 from: 1 to: 666
KIEE3067.orf
With 182 enzymes: SgfI *
March 22, 2012 16:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 666
Size: 545 121
AflIII A'CryG_T
Cuts at: 0 330 666
Size: 330 336
ApaI G_GGCC'C
Cuts at: 0 148 444 482 510 552 562 666
Size: 148 296 38 28 42 10 104
Fragments arranged by size:
296 148 104 42 38 28 10
AvaI C'yCGr_G
Cuts at: 0 373 437 561 666
Size: 373 64 124 105
Fragments arranged by size:
373 124 105 64
BanI G'GyrC_C
Cuts at: 0 265 469 666
Size: 265 204 197
Fragments arranged by size:
265 204 197
BanII G_rGCy'C
Cuts at: 0 148 444 482 510 552 562 666
Size: 148 296 38 28 42 10 104
Fragments arranged by size:
296 148 104 42 38 28 10
BbeI G_GCGC'C
Cuts at: 0 269 473 666
Size: 269 204 193
Fragments arranged by size:
269 204 193
BbvCI CC'TCA_GC
Cuts at: 0 572 666
Size: 572 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 412 446 666
Size: 412 34 220
Fragments arranged by size:
412 220 34
BglI GCCn_nnn'nGGC
Cuts at: 0 505 666
Size: 505 161
BglII A'GATC_T
Cuts at: 0 245 278 666
Size: 245 33 388
Fragments arranged by size:
388 245 33
BlpI GC'TnA_GC
Cuts at: 0 314 666
Size: 314 352
Bme1580I G_kGCm'C
Cuts at: 0 148 444 482 510 552 562 666
Size: 148 296 38 28 42 10 104
Fragments arranged by size:
296 148 104 42 38 28 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 188 666
Size: 112 76 478
Fragments arranged by size:
478 112 76
Bpu10I CC'TnA_GC
Cuts at: 0 572 666
Size: 572 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 666
Size: 280 386
BsaAI yAC'GTr
Cuts at: 0 333 666
Size: 333 333
BsaHI Gr'CG_yC
Cuts at: 0 266 470 666
Size: 266 204 196
Fragments arranged by size:
266 204 196
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 406 439 666
Size: 124 282 33 227
Fragments arranged by size:
282 227 124 33
BseYI C'CCAG_C
Cuts at: 0 195 318 481 666
Size: 195 123 163 185
Fragments arranged by size:
195 185 163 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 666
Size: 365 301
BsiEI CG_ry'CG
Cuts at: 0 504 666
Size: 504 162
BsiHKAI G_wGCw'C
Cuts at: 0 296 666
Size: 296 370
Bsp1286I G_dGCh'C
Cuts at: 0 148 296 444 482 510 552 562 666
Size: 148 148 148 38 28 42 10 104
Fragments arranged by size:
148 148 148 104 42 38 28 10
BsrFI r'CCGG_y
Cuts at: 0 472 499 666
Size: 472 27 167
Fragments arranged by size:
472 167 27
BsrGI T'GTAC_A
Cuts at: 0 47 666
Size: 47 619
BstEII G'GTnAC_C
Cuts at: 0 132 666
Size: 132 534
BstYI r'GATC_y
Cuts at: 0 245 278 458 666
Size: 245 33 180 208
Fragments arranged by size:
245 208 180 33
Bsu36I CC'TnA_GG
Cuts at: 0 149 666
Size: 149 517
BtgI C'CryG_G
Cuts at: 0 462 581 666
Size: 462 119 85
Fragments arranged by size:
462 119 85
DrdI GACnn_nn'nnGTC
Cuts at: 0 130 666
Size: 130 536
EaeI y'GGCC_r
Cuts at: 0 512 584 623 666
Size: 512 72 39 43
Fragments arranged by size:
512 72 43 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 666
Size: 28 638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 188 545 666
Size: 112 76 357 121
Fragments arranged by size:
357 121 112 76
EcoO109I rG'GnC_Cy
Cuts at: 0 114 144 145 155 507 548 549 558
Size: 114 30 1 10 352 41 1 9
Cuts at: 558 577 666
Size: 19 89
Fragments arranged by size:
352 114 89 41 30 19 10 9
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 249 666
Size: 249 417
HaeII r_GCGC'y
Cuts at: 0 269 473 655 666
Size: 269 204 182 11
Fragments arranged by size:
269 204 182 11
HincII GTy'rAC
Cuts at: 0 288 666
Size: 288 378
HindIII A'AGCT_T
Cuts at: 0 527 666
Size: 527 139
KasI G'GCGC_C
Cuts at: 0 265 469 666
Size: 265 204 197
Fragments arranged by size:
265 204 197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 351 666
Size: 32 319 315
Fragments arranged by size:
319 315 32
MscI TGG'CCA
Cuts at: 0 514 586 666
Size: 514 72 80
Fragments arranged by size:
514 80 72
MspA1I CmG'CkG
Cuts at: 0 485 666
Size: 485 181
NaeI GCC'GGC
Cuts at: 0 474 666
Size: 474 192
NarI GG'CG_CC
Cuts at: 0 266 470 666
Size: 266 204 196
Fragments arranged by size:
266 204 196
NcoI C'CATG_G
Cuts at: 0 581 666
Size: 581 85
NdeI CA'TA_TG
Cuts at: 0 162 666
Size: 162 504
NgoMIV G'CCGG_C
Cuts at: 0 472 666
Size: 472 194
NspI r_CATG'y
Cuts at: 0 598 666
Size: 598 68
PasI CC'CwG_GG
Cuts at: 0 544 553 666
Size: 544 9 113
Fragments arranged by size:
544 113 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 85 166 666
Size: 85 81 500
Fragments arranged by size:
500 85 81
PpuMI rG'GwC_Cy
Cuts at: 0 114 155 577 666
Size: 114 41 422 89
Fragments arranged by size:
422 114 89 41
PspOMI G'GGCC_C
Cuts at: 0 144 440 478 506 548 558 666
Size: 144 296 38 28 42 10 108
Fragments arranged by size:
296 144 108 42 38 28 10
SanDI GG'GwC_CC
Cuts at: 0 155 577 666
Size: 155 422 89
Fragments arranged by size:
422 155 89
SfoI GGC'GCC
Cuts at: 0 267 471 666
Size: 267 204 195
Fragments arranged by size:
267 204 195
SgrAI Cr'CCGG_yG
Cuts at: 0 472 666
Size: 472 194
SmaI CCC'GGG
Cuts at: 0 563 666
Size: 563 103
SmlI C'TyrA_G
Cuts at: 0 295 666
Size: 295 371
SphI G_CATG'C
Cuts at: 0 598 666
Size: 598 68
StyI C'CwwG_G
Cuts at: 0 224 581 666
Size: 224 357 85
Fragments arranged by size:
357 224 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 237 666
Size: 237 429
TatI w'GTAC_w
Cuts at: 0 47 666
Size: 47 619
XmaI C'CCGG_G
Cuts at: 0 561 666
Size: 561 105
XmnI GAAnn'nnTTC
Cuts at: 0 205 666
Size: 205 461
Enzymes that do cut:
AcuI AflIII ApaI AvaI BanI BanII BbeI BbvCI
BcgI BglI BglII BlpI Bme1580I BpmI Bpu10I BpuEI
BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BsrFI BsrGI BstEII BstYI Bsu36I BtgI DrdI EaeI
EciI Eco57MI EcoO109I FalI HaeII HincII HindIII KasI
MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PflMI PpuMI PspOMI SanDI SfoI SgrAI
SmaI SmlI SphI StyI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI
BfrBI BmgBI BmrI BmtI BplI BsaI BsaBI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII EagI EarI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HpaI
KpnI MfeI MluI MslI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI ZraI