(Linear) (Six-base) MAPSORT of: KIEE3066.seq  Check: 5772  from: 1  to: 663



KIEE3066.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:09  ..

AccI GT'mk_AC

Cuts at:      0     322     663 
   Size:        322     341
 
AflIII A'CryG_T

Cuts at:      0      88     663 
   Size:         88     575
 
AlwNI CAG_nnn'CTG

Cuts at:      0     366     663 
   Size:        366     297
 
ApoI r'AATT_y

Cuts at:      0     194     306     663 
   Size:        194     112     357

  Fragments arranged by size: 

                 357     194     112
 
BanI G'GyrC_C

Cuts at:      0      61     663 
   Size:         61     602
 
BbeI G_GCGC'C

Cuts at:      0      65     663 
   Size:         65     598
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      79     489     663 
   Size:         79     410     174

  Fragments arranged by size: 

                 410     174      79
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     663 
   Size:         83     580
 
BsaAI yAC'GTr

Cuts at:      0      89     663 
   Size:         89     574
 
BsaHI Gr'CG_yC

Cuts at:      0      62     663 
   Size:         62     601
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      49      79     479     509     663 
   Size:         49      30     400      30     154

  Fragments arranged by size: 

                 400     154      49      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      28      31     361     663 
   Size:         28       3     330     302

  Fragments arranged by size: 

                 330     302      28       3
 
BseYI C'CCAG_C

Cuts at:      0      51     663 
   Size:         51     612
 
BsiHKAI G_wGCw'C

Cuts at:      0     258     663 
   Size:        258     405
 
Bsp1286I G_dGCh'C

Cuts at:      0     258     663 
   Size:        258     405
 
BsrDI GCAATG_nn'

Cuts at:      0     163     663 
   Size:        163     500
 
BsrFI r'CCGG_y

Cuts at:      0      29     663 
   Size:         29     634
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     451     663 
   Size:        451     212
 
BstZ17I GTA'TAC

Cuts at:      0     323     663 
   Size:        323     340
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      96     663 
   Size:         96     567
 
HaeII r_GCGC'y

Cuts at:      0      65     663 
   Size:         65     598
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      49      81     479     511     663 
   Size:         49      32     398      32     152

  Fragments arranged by size: 

                 398     152      49      32      32
 
HincII GTy'rAC

Cuts at:      0     630     663 
   Size:        630      33
 
HindIII A'AGCT_T

Cuts at:      0     297     663 
   Size:        297     366
 
HpaI GTT'AAC

Cuts at:      0     630     663 
   Size:        630      33
 
KasI G'GCGC_C

Cuts at:      0      61     663 
   Size:         61     602
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     663 
   Size:        215     448
 
NarI GG'CG_CC

Cuts at:      0      62     663 
   Size:         62     601
 
NdeI CA'TA_TG

Cuts at:      0     404     663 
   Size:        404     259
 
PfoI T'CCnGG_A

Cuts at:      0      67     663 
   Size:         67     596
 
SfcI C'TryA_G

Cuts at:      0     131     663 
   Size:        131     532
 
SfoI GGC'GCC

Cuts at:      0      63     663 
   Size:         63     600
 
SmlI C'TyrA_G

Cuts at:      0      98     663 
   Size:         98     565
 
StyI C'CwwG_G

Cuts at:      0     374     531     663 
   Size:        374     157     132

  Fragments arranged by size: 

                 374     157     132
 
TatI w'GTAC_w

Cuts at:      0     119     663 
   Size:        119     544
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     248     280     663 
   Size:        248      32     383

  Fragments arranged by size: 

                 383     248      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     493     663 
   Size:        493     170
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI     ApoI     BanI     BbeI     BbsI    BpuEI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BsrDI
    BsrFI    BstXI  BstZ17I     FalI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     MmeI     NarI     NdeI     PfoI     SfcI     SfoI
     SmlI     StyI     TatI     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaWI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI